Hepatic differentiation of pluripotent stem cells
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Komal Loya
Abstract
In regenerative medicine pluripotent stem cells are considered to be a valuable self-renewing source for therapeutic cell transplantations, given that a functional organ-specific phenotype can be acquired by in vitro differentiation protocols. Furthermore, derivatives of pluripotent stem cells that mimic fetal progenitor stages could serve as an important tool to analyze organ development with in vitro approaches. Because of ethical issues regarding the generation of human embryonic stem (ES) cells, other sources for pluripotent stem cells are intensively studied. Like in less developed vertebrates, pluripotent stem cells can be generated from the female germline even in mammals, via parthenogenetic activation of oocytes. Recently, testis-derived pluripotent stem cells were derived from the male germline. Therefore, we compared two different hepatic differentiation approaches and analyzed the generation of definitive endoderm progenitor cells and their further maturation into a hepatic phenotype using murine parthenogenetic ES cells, germline-derived pluripotent stem cells, and ES cells. Applying quantitative RT-PCR, both germline-derived pluripotent cell lines show similar differentiation capabilities as normal murine ES cells and can be considered an alternative source for pluripotent stem cells in regenerative medicine.
©2009 by Walter de Gruyter Berlin New York
Artikel in diesem Heft
- Guest Editorial
- Highlight: ‘Regenerative Hepatology’
- Highlight: Regenerative Hepatology
- The do's and don'ts of p53 isoforms
- Mechanisms of liver disease: cross-talk between the NF-κB and JNK pathways
- Immunologic hurdles of therapeutic stem cell transplantation
- Ancestral vascular tube formation and its adoption by tumors
- Cellular plasticity of the pancreas
- Hepatic and pancreatic stellate cells in focus
- Interplay between host cell and hepatitis C virus in regulating viral replication
- Epidermal growth factor receptor signaling in liver cell proliferation and apoptosis
- The chemokine scavenging receptor D6 limits acute toxic liver injury in vivo
- Hepatic differentiation of pluripotent stem cells
- Protein Structure and Function
- Effect of curcumin on amyloidogenic property of molten globule-like intermediate state of 2,5-diketo-d-gluconate reductase A
- Cell Biology and Signaling
- Specific induction of migration and invasion of pancreatic carcinoma cells by RhoC, which differs from RhoA in its localisation and activity
- Autoregulatory control of the p53 response by Siah-1L-mediated HIPK2 degradation
Artikel in diesem Heft
- Guest Editorial
- Highlight: ‘Regenerative Hepatology’
- Highlight: Regenerative Hepatology
- The do's and don'ts of p53 isoforms
- Mechanisms of liver disease: cross-talk between the NF-κB and JNK pathways
- Immunologic hurdles of therapeutic stem cell transplantation
- Ancestral vascular tube formation and its adoption by tumors
- Cellular plasticity of the pancreas
- Hepatic and pancreatic stellate cells in focus
- Interplay between host cell and hepatitis C virus in regulating viral replication
- Epidermal growth factor receptor signaling in liver cell proliferation and apoptosis
- The chemokine scavenging receptor D6 limits acute toxic liver injury in vivo
- Hepatic differentiation of pluripotent stem cells
- Protein Structure and Function
- Effect of curcumin on amyloidogenic property of molten globule-like intermediate state of 2,5-diketo-d-gluconate reductase A
- Cell Biology and Signaling
- Specific induction of migration and invasion of pancreatic carcinoma cells by RhoC, which differs from RhoA in its localisation and activity
- Autoregulatory control of the p53 response by Siah-1L-mediated HIPK2 degradation
