Krüppel-like factor 4 represses transcription of the survivin gene in esophageal cancer cell lines
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Abstract
Aberrant expression of survivin has been shown to be regulated at the transcription level in cancer cells. In this study, we demonstrate that there are six putative binding sites of Krüppel-like factor 4 (KLF4) within the 2000-bp region upstream of the transcription start site of the human survivin gene. Luciferase reporter gene assays revealed that survivin promoter activity is repressed upon overexpression of KLF4 in EC9706 cells. A chromatin immunoprecipitation assay indicated that KLF4 indeed binds the survivin promoter in vivo. It specifically binds the site located at position -40 among the six binding sites as determined by electrophoretic mobility shift assay. Ectopic expression of KLF4 decreases the mRNA and protein levels of survivin. Furthermore, overexpression of survivin partially reverses KLF4-induced cell apoptosis. These results indicate that KLF4 is a transcriptional repressor of the human survivin gene in esophageal squamous cancer cells.
©2009 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Reviews
- The liaison between apoptotic cells and macrophages – the end programs the beginning
- Activation mechanisms of coagulation factor IX
- Subsite cooperativity in protease specificity
- Minireview
- Retrodifferentiation – a mechanism for cellular regeneration?
- Protein Structure and Function
- The solution structure of pGolemi, a high affinity Mena EVH1 binding miniature protein, suggests explanations for paralog-specific binding to Ena/VASP homology (EVH) 1 domains
- 3D structure of a binary ROP-PRONE complex: the final intermediate for a complete set of molecular snapshots of the RopGEF reaction
- Identification and characterization of a bioactive lantibiotic produced by Staphylococcus warneri
- Role of the polypeptide backbone and post-translational modifications in cross-reactivity of Art v 1, the major mugwort pollen allergen
- Cell Biology and Signaling
- Short double-stranded RNAs of specific sequence activate ribosomal TAK1-D and induce a global inhibition of translation
- Krüppel-like factor 4 represses transcription of the survivin gene in esophageal cancer cell lines
- Proteolysis
- Release of endo-lysosomal cathepsins B, D, and L from IEC6 cells in a cell culture model mimicking intestinal manipulation
- Cathepsin B release from rodent intestine mucosa due to mechanical injury results in extracellular matrix damage in early post-traumatic phases
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- Cooperative effects in the substrate specificity of the complement protease C1s
- Novel Techniques
- The influenza A virus matrix protein as a marker to monitor initial virus internalisation
- Erratum
- Murine and human cathepsin B exhibit similar properties: possible implications for drug discovery
