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The PAK1 autoregulatory domain is required for interaction with NIK in Helicobacter pylori-induced NF-κB activation

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Veröffentlicht/Copyright: 13. Januar 2006
Biological Chemistry
Aus der Zeitschrift Band 387 Heft 1

Abstract

Helicobacter pylori, the etiological agent of various human gastric diseases, induces the transcription factor nuclear factor κB (NF-κB) and proinflammatory cytokines/chemokines. We have characterised the direct interaction between p21-activated kinase 1 (PAK1) and NF-κB-inducing kinase (NIK) in H. pylori-infected epithelial cells. The dimerisation (DI) motif, which is part of the NH2-terminal autoregulatory domain of PAK1, is critical for this interaction, whereas NIK forms complexes with PAK1 through its carboxy-terminal IκB kinase α (IKKα) binding site. Since the identified interaction sites are also crucial for the binding of activator (Rac/Cdc42 in the case of PAK1) or effector molecules (IKKα in the case of NIK), sequential stepwise signalling is suggested. Furthermore, we show that mitogen-activated protein kinase kinase kinases (MAP3K), like TPL2 (tumour progression locus 2) and transforming growth factor β-activated kinase 1 (TAK1), have no impact on H. pylori-induced activation of NF-κB. These results identify the roles of PAK1 and NIK in a unique pathway involved in H. pylori-induced NF-κB activation, which is crucial for the induction of the innate immune response.

Keywords: epithelial cells; IκB; IκB kinases; innate immune response; pathogenicity island; type 4 secretion system
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Corresponding author

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Published Online: 2006-01-13
Published in Print: 2006-01-01

©2006 by Walter de Gruyter Berlin New York

Artikel in diesem Heft

  1. Editor's Note
  2. Endothelial mediators and communication through vascular gap junctions
  3. Bradykinin and peripheral sensitization
  4. Renal gene expression profiling using kinin B1 and B2 receptor knockout mice reveals comparable modulation of functionally related genes
  5. Solvent-induced changes in photochemical activity and conformation of photosystem I particles by glycerol
  6. Interaction of the BELL-like protein ATH1 with DNA: role of homeodomain residue 54 in specifying the different binding properties of BELL and KNOX proteins
  7. Vitamin B1de novo synthesis in the human malaria parasite Plasmodium falciparum depends on external provision of 4-amino-5-hydroxymethyl-2-methylpyrimidine
  8. Ecto- and cytosolic 5′-nucleotidases in normal and AMP deaminase-deficient human skeletal muscle
  9. Dual signal transduction mediated by a single type of olfactory receptor expressed in a heterologous system
  10. Modulation of autocrine TNF-α-stimulated matrix metalloproteinase 9 (MMP-9) expression by mitogen-activated protein kinases in THP-1 monocytic cells
  11. The PAK1 autoregulatory domain is required for interaction with NIK in Helicobacter pylori-induced NF-κB activation
  12. Aflatoxin B1-induced toxicity in HepG2 cells inhibited by carotenoids: morphology, apoptosis and DNA damage
  13. Detection of prion particles in samples of BSE and scrapie by fluorescence correlation spectroscopy without proteinase K digestion
  14. A method to determine RNA and DNA oxidation simultaneously by HPLC-ECD: greater RNA than DNA oxidation in rat liver after doxorubicin administration
  15. NF-κB contributes to transcription of placenta growth factor and interacts with metal responsive transcription factor-1 in hypoxic human cells
https://doi.org/10.1515/BC.2006.011
Schlagwörter für diesen Artikel
epithelial cells; IκB; IκB kinases; innate immune response; pathogenicity island; type 4 secretion system

Artikel in diesem Heft

  1. Editor's Note
  2. Endothelial mediators and communication through vascular gap junctions
  3. Bradykinin and peripheral sensitization
  4. Renal gene expression profiling using kinin B1 and B2 receptor knockout mice reveals comparable modulation of functionally related genes
  5. Solvent-induced changes in photochemical activity and conformation of photosystem I particles by glycerol
  6. Interaction of the BELL-like protein ATH1 with DNA: role of homeodomain residue 54 in specifying the different binding properties of BELL and KNOX proteins
  7. Vitamin B1de novo synthesis in the human malaria parasite Plasmodium falciparum depends on external provision of 4-amino-5-hydroxymethyl-2-methylpyrimidine
  8. Ecto- and cytosolic 5′-nucleotidases in normal and AMP deaminase-deficient human skeletal muscle
  9. Dual signal transduction mediated by a single type of olfactory receptor expressed in a heterologous system
  10. Modulation of autocrine TNF-α-stimulated matrix metalloproteinase 9 (MMP-9) expression by mitogen-activated protein kinases in THP-1 monocytic cells
  11. The PAK1 autoregulatory domain is required for interaction with NIK in Helicobacter pylori-induced NF-κB activation
  12. Aflatoxin B1-induced toxicity in HepG2 cells inhibited by carotenoids: morphology, apoptosis and DNA damage
  13. Detection of prion particles in samples of BSE and scrapie by fluorescence correlation spectroscopy without proteinase K digestion
  14. A method to determine RNA and DNA oxidation simultaneously by HPLC-ECD: greater RNA than DNA oxidation in rat liver after doxorubicin administration
  15. NF-κB contributes to transcription of placenta growth factor and interacts with metal responsive transcription factor-1 in hypoxic human cells
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