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Plasma prekallikrein/kallikrein processing by lysosomal cysteine proteases

  • Nilana M.T. Barros , Luciano Puzer , Ivarne L.S. Tersariol , M. Luiza V. Oliva , Claudio A.M. Sampaio , Adriana K. Carmona and Guacyara Da Motta
Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 385 Issue 11

Abstract

Plasma kallikrein plays a role in coagulation, fibrinolysis and inflammation. Cathepsins B and L participate in (patho)physiological processes such as peptide antigen processing, tissue remodeling events, protein turnover in cells, hormone processing and tumor invasion. The present work analyzes the processing of prekallikrein/kallikrein by lysosomal cathepsins. Prekallikrein is not hydrolyzed by catB, and catL generates an inactive fragment of prekallikrein. Both kallikrein chains are hydrolyzed by catL and the light chain is mainly hydrolyzed by catB; kallikrein activity is lower after incubation with catL compared to catB. Our data suggest that the plasma kallikrein/ kinin system can be controlled by cathepsins.

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Published Online: 2005-06-01
Published in Print: 2004-11-01

© Walter de Gruyter

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