Artikel
Lizenziert
Nicht lizenziert
Erfordert eine Authentifizierung
A Simple Single-Step Method for the Synthesis of Recombinant Non-Homologous Competitor cDNA and Its Implications for Quantitative PCR in Biological Sciences and Molecular Diagnostics
-
P. K. Grover
Veröffentlicht/Copyright:
1. Juni 2005
Abstract
In recent years there has been great interest in quantitative polymerase chain reaction. Consequently, a large number of assays have been developed, of which the one using nonhomologous competitors is arguably the most precise. Despite widespread applications, currently there is no simple method to synthesize such competitors. Here a facile and costeffective, singlestep method for synthesis of recombinant, nonhomologous, competitor complementary DNA is described. The method can be adapted to generate competitors of any size and sequence. The entire procedure is quick, straightforward and does not require any specialized equipment except a standard thermocycler.
:
Published Online: 2005-06-01
Published in Print: 2002-09-17
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Artikel in diesem Heft
- Kaposis Sarcoma and Human Chorionic Gonadotropin: Mechanisms, Moieties and Mysteries
- Dimerization Inhibitors of HIV-1 Protease
- A Dimeric Mutant of the Homotetrameric Single-Stranded DNA Binding Protein from Escherichia coli
- The Hsp90 Co-Chaperones Cdc37 and Sti1 Interact Physically and Genetically
- Cloning and Sequence Analysis of Galleria mellonella Juvenile Hormone Binding Protein A Search for Ancestors and Relatives
- Neuronal NO Synthase and Its Inhibitor PIN Are Present and Influenced by Glucose in the Human β-Cell Line CM and in Rat INS-1 Cells
- Enzymatic Activity of the Arabidopsis Sulfurtransferase Resides in the C-Terminal Domain But Is Boosted by the N-Terminal Domain and the Linker Peptide in the Full- Length Enzyme
- The in vitro Assembly of Hair Follicle Keratins: Comparison of Cortex and Companion Layer Keratins
- Purification, Structural and Immunological Characterization of a Timothy Grass (Phleum pratense) Pollen Allergen, Phl p 4, with Cross-Reactive Potential
- Muscarinic Toxin-Like Proteins from Taiwan Banded Krait (Bungarus multicinctus) Venom: Purification, Characterization and Gene Organization
- Degradation of Extracellular Matrix Protein Tenascin-C by Cathepsin B: An Interaction Involved in the Progression of Gliomas
- Flexibility Analysis and Structure Comparison of Two Crystal Forms of Calcium-Free Human m-Calpain
- Detection and Quantification of Insertion/Deletion Variations by Allele-Specific Real-Time PCR: Application for Genotyping and Chimerism Analysis
- Reconstitution of Phospholipid Flippase Activity from E. coli Inner Membrane: A Test of the Protein Translocon as a Candidate Flippase
- Identification of an Allergen Related to Phl p 4, a Major Timothy Grass Pollen Allergen, in Pollens, Vegetables, and Fruits by Immunogold Electron Microscopy
- Subunit Association and Conformational Flexibility in the Head Subdomain of Human CD81 Large Extracellular Loop
- Foldase Function of the Cathepsin S Proregion Is Strictly Based upon Its Domain Structure
- An Efficient Method for the Preparation of Long Heteroduplex DNA as Substrate for Mismatch Repair by the Escherichia coli MutHLS System
- A Simple Single-Step Method for the Synthesis of Recombinant Non-Homologous Competitor cDNA and Its Implications for Quantitative PCR in Biological Sciences and Molecular Diagnostics
Artikel in diesem Heft
- Kaposis Sarcoma and Human Chorionic Gonadotropin: Mechanisms, Moieties and Mysteries
- Dimerization Inhibitors of HIV-1 Protease
- A Dimeric Mutant of the Homotetrameric Single-Stranded DNA Binding Protein from Escherichia coli
- The Hsp90 Co-Chaperones Cdc37 and Sti1 Interact Physically and Genetically
- Cloning and Sequence Analysis of Galleria mellonella Juvenile Hormone Binding Protein A Search for Ancestors and Relatives
- Neuronal NO Synthase and Its Inhibitor PIN Are Present and Influenced by Glucose in the Human β-Cell Line CM and in Rat INS-1 Cells
- Enzymatic Activity of the Arabidopsis Sulfurtransferase Resides in the C-Terminal Domain But Is Boosted by the N-Terminal Domain and the Linker Peptide in the Full- Length Enzyme
- The in vitro Assembly of Hair Follicle Keratins: Comparison of Cortex and Companion Layer Keratins
- Purification, Structural and Immunological Characterization of a Timothy Grass (Phleum pratense) Pollen Allergen, Phl p 4, with Cross-Reactive Potential
- Muscarinic Toxin-Like Proteins from Taiwan Banded Krait (Bungarus multicinctus) Venom: Purification, Characterization and Gene Organization
- Degradation of Extracellular Matrix Protein Tenascin-C by Cathepsin B: An Interaction Involved in the Progression of Gliomas
- Flexibility Analysis and Structure Comparison of Two Crystal Forms of Calcium-Free Human m-Calpain
- Detection and Quantification of Insertion/Deletion Variations by Allele-Specific Real-Time PCR: Application for Genotyping and Chimerism Analysis
- Reconstitution of Phospholipid Flippase Activity from E. coli Inner Membrane: A Test of the Protein Translocon as a Candidate Flippase
- Identification of an Allergen Related to Phl p 4, a Major Timothy Grass Pollen Allergen, in Pollens, Vegetables, and Fruits by Immunogold Electron Microscopy
- Subunit Association and Conformational Flexibility in the Head Subdomain of Human CD81 Large Extracellular Loop
- Foldase Function of the Cathepsin S Proregion Is Strictly Based upon Its Domain Structure
- An Efficient Method for the Preparation of Long Heteroduplex DNA as Substrate for Mismatch Repair by the Escherichia coli MutHLS System
- A Simple Single-Step Method for the Synthesis of Recombinant Non-Homologous Competitor cDNA and Its Implications for Quantitative PCR in Biological Sciences and Molecular Diagnostics
