The Small GTPase Ras Is Involved in Growth Factor-Regulated Expression of the α1 Integrin Subunit in PC12 Cells
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Kerstin Danker
Abstract
PC12 cells interact with several growth factors (e. g. EGF, FGF, and NGF) via specific tyrosine receptor kinases, resulting in cell proliferation or neuronal differentiation. The small GTPase Ras is known to be involved in downstream signaling of these growth factor receptors. Furthermore, cellmatrix interactions mediated by integrins, as well as integrininduced signaling, are also involved in growth factorstimulated signal transduction in PC12 cells. In this study we determined the expression of the α1 integrin subunit in response to EGF and NGF in PC12 wildtype (wt) cells, and in PC12 cells overexpressing an inactive HRas protein (RasN17). In PC12 wt cells, α1 integrin expression is upregulated by EGF and NGF. Cell surface expression of α1β1 integrin is also enhanced in growth factortreated cells. This upregulation leads to increased α1β1- specific adhesion to collagen. In cells expressing the dominantnegative RasN17 variant, α1 integrin expression and α1β1-specific adhesion remain unchanged in response to both growth factors.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Biological Activity of Mammalian Transcriptional Repressors
- Cloning and Characterisation of Chlorophyll Synthase from Avena sativa
- DNA Damage by 3,6-Dihydropyrazine-2,5-Dipropanoic Acid, the Cyclic Dimerization Product of 5-Aminolevulinic Acid
- Chicken Erythrocyte Pyrimidine 5-Nucleotidase: Purification and Characterization of the Subclass I Enzyme
- Coenzymes Q9 and Q10, Vitamin E and Peroxidation in Rat Synaptic and Non-Synaptic Occipital Cerebral Cortex Mitochondria during Ageing
- Membrane Activity of (Cys48Ser) Lung Surfactant Protein B Increases with Dimerisation
- Participation of Residue F552 in Domain III of the Protective Antigen in the Biological Activity of Anthrax Lethal Toxin
- Purification of Chrysancorin, a Novel Antifungal Protein with Mitogenic Activity from Garland Chrysanthemum Seeds
- Legumain Forms from Plants and Animals Differ in Their Specificity
- Assignment of the Complete Disulphide Bridge Pattern in the Human Recombinant Follitropin β -Chain
- The Small GTPase Ras Is Involved in Growth Factor-Regulated Expression of the α1 Integrin Subunit in PC12 Cells
- The Mouse Gap Junction Gene Connexin29 Is Highly Expressed in Sciatic Nerve and Regulated during Brain Development
- A Selenocysteine-Containing Peroxiredoxin from the Strictly Anaerobic Organism Eubacterium acidaminophilum
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