Molecular Cloning and Pharmacological Characterization of the Canine B1 and B2 Bradykinin Receptors
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J. Fred Hess
Abstract
The dog is a valuable animal model in the study of the physiological role of both the B1 and B2 bradykinin receptors. To more thoroughly characterize the pharmacological properties of the canine kinin receptors we isolated the cDNA sequence encoding the B1 and B2 bradykinin receptor subtypes and overexpressed them in Chinese hamster ovary (CHO) cells. The cDNA sequence of the canine B1 bradykinin receptor encodes a protein comprised of 350 amino acids that is 76% identical to the human B1 bradykinin receptor. The cDNA sequence of the canine B2 bradykinin receptor encodes a protein of 392 amino acids that is 81% identical to the human B2 bradykinin receptor. The amino acid sequence of the canine B1 and B2 receptors are 35% identical. Pharmacological studies of the cloned receptors revealed that the agonist affinity of the dog B1 receptor is similar to the rodent B1 receptors, and differs from the human form in that there is no preference for the presence of the Nterminal Lys residue of [desArg10]Lysbradykinin. Significantly, the B1 receptor antagonist [desArg9,Leu8]BK behaves as partial agonist on the cloned dog B1 receptor. The dog B2 receptor exhibits the classical pharmacological properties of this receptor subtype.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- To Our Authors, Readers and Subscribers
- Kinins 1925-2000
- The Expanded Human Kallikrein (KLK) Gene Family: Genomic Organisation, Tissue-Specific Expression and Potential Functions
- Novel Roles of Kallistatin, a Specific Tissue Kallikrein Inhibitor, in Vascular Remodeling
- Signal Transduction from Bradykinin, Angiotensin, Adrenergic and Muscarinic Receptors to Effector Enzymes, Including ADP-Ribosyl Cyclase
- Classification of Kinin Receptors
- Metabolism-Resistant Bradykinin Antagonists: Development and Applications
- Kinins, Receptors, Kininases and Inhibitors Where Did They Lead Us?
- Bradykinin Signalling to MAP Kinase: Cell-Specific Connections versus Principle Mitogenic Pathways
- Kinins and Epithelial Ion Transport in the Alimentary Tract
- Role of the Renal Kallikrein-Kinin System in the Development of Salt-Sensitive Hypertension
- Role of the Light Chain of High Molecular Weight Kininogen in Adhesion, Cell-Associated Proteolysis and Angiogenesis
- Activation of the Kinin-Forming Cascade on the Surface of Endothelial Cells
- Kallikrein and Kinin Receptor Expression in Inflammation and Cancer
- Altered Neutrophil Homeostasis in Kinin B1 Receptor-Deficient Mice
- Cystatins as Calpain Inhibitors: Engineered Chicken Cystatin- and Stefin B-Kininogen Domain 2 Hybrids Support a Cystatin-Like Mode of Interaction with the Catalytic Subunit of µ-Calpain
- Synthetic Peptides and Fluorogenic Substrates Related to the Reactive Site Sequence of Kunitz-Type Inhibitors Isolated from Bauhinia: Interaction with Human Plasma Kallikrein
- Identification and Characterization of an Aromatic Amino Acid Decarboxylase from the Filarial Nematode, Dirofilaria immitis
- Molecular Cloning and Pharmacological Characterization of the Canine B1 and B2 Bradykinin Receptors
- Ligand-Mediated Regulation of Kinin Receptors in the Rabbit
- Activation of Sphingosine Kinase by the Bradykinin B2 Receptor and Its Implication in Regulation of the ERK/MAP Kinase Pathway
Articles in the same Issue
- To Our Authors, Readers and Subscribers
- Kinins 1925-2000
- The Expanded Human Kallikrein (KLK) Gene Family: Genomic Organisation, Tissue-Specific Expression and Potential Functions
- Novel Roles of Kallistatin, a Specific Tissue Kallikrein Inhibitor, in Vascular Remodeling
- Signal Transduction from Bradykinin, Angiotensin, Adrenergic and Muscarinic Receptors to Effector Enzymes, Including ADP-Ribosyl Cyclase
- Classification of Kinin Receptors
- Metabolism-Resistant Bradykinin Antagonists: Development and Applications
- Kinins, Receptors, Kininases and Inhibitors Where Did They Lead Us?
- Bradykinin Signalling to MAP Kinase: Cell-Specific Connections versus Principle Mitogenic Pathways
- Kinins and Epithelial Ion Transport in the Alimentary Tract
- Role of the Renal Kallikrein-Kinin System in the Development of Salt-Sensitive Hypertension
- Role of the Light Chain of High Molecular Weight Kininogen in Adhesion, Cell-Associated Proteolysis and Angiogenesis
- Activation of the Kinin-Forming Cascade on the Surface of Endothelial Cells
- Kallikrein and Kinin Receptor Expression in Inflammation and Cancer
- Altered Neutrophil Homeostasis in Kinin B1 Receptor-Deficient Mice
- Cystatins as Calpain Inhibitors: Engineered Chicken Cystatin- and Stefin B-Kininogen Domain 2 Hybrids Support a Cystatin-Like Mode of Interaction with the Catalytic Subunit of µ-Calpain
- Synthetic Peptides and Fluorogenic Substrates Related to the Reactive Site Sequence of Kunitz-Type Inhibitors Isolated from Bauhinia: Interaction with Human Plasma Kallikrein
- Identification and Characterization of an Aromatic Amino Acid Decarboxylase from the Filarial Nematode, Dirofilaria immitis
- Molecular Cloning and Pharmacological Characterization of the Canine B1 and B2 Bradykinin Receptors
- Ligand-Mediated Regulation of Kinin Receptors in the Rabbit
- Activation of Sphingosine Kinase by the Bradykinin B2 Receptor and Its Implication in Regulation of the ERK/MAP Kinase Pathway
