Article
Licensed
Unlicensed
Requires Authentication
Superoxide Reactivates Nitric Oxide-Inhibited Catalase
-
Yu Shin Kim
Published/Copyright:
June 1, 2005
Abstract
Catalase binds nitric oxide (NO) to generate ferricatalase-NO, an inhibited form of the enzyme. Superoxide (O2−) is also an inactivator of the enzyme. We found, however, that O2− efficiently converted the inhibited ferricatalase-NO to the active ferricatalase without producing detectable intermediates. The reaction slowed down when O2− was disproportionated to H2O2 and O2 by superoxide dismutase, but H2O2 could displace the heme-bound NO slowly to regenerate ferricatalase. Reactivation was observed even under simultaneous generation of NO and O2− suggesting that ferricatalase-NO reacts with O2− fast enough to compete with the rapid reaction of O2− and NO. Formation of peroxynitrite by the simultaneous generation of NO and O2− was only partially inhibited by ferricatalase, presumably due to slow binding of NO to catalase in comparison with the reaction of NO and O2−.
:
Published Online: 2005-06-01
Published in Print: 2000-12-18
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Circadian Rhythms of Sterol 12α-Hydroxylase, Cholesterol 7α-Hydroxylase and DBP Involved in Rat Cholesterol Catabolism
- Mannosidase Action, Independent of Glucose Trimming, Is Essential for Proteasome-Mediated Degradation of Unassembled Glycosylated Ig Light Chains
- Characterization of a Receptor for Heat Shock Protein 70 on Macrophages and Monocytes
- Yeast Translational Activator Cbs2p: Mitochondrial Targeting and Effect of Overexpression
- Mutational Scanning of a Hairpin Loop in the Tryptophan Synthase β-Subunit Implicated in Allostery and Substrate Channeling
- The Difference in the Carboxy-Terminal Sequence Is Responsible for the Difference in the Activity of Chicken and Rat Liver Fructose-2,6-Bisphosphatase
- Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue
- Primary Structure of Potato Kunitz-Type Serine Proteinase Inhibitor
- Activation of proPHBSP, the Zymogen of a Plasma Hyaluronan Binding Serine Protease, by an Intermolecular Autocatalytic Mechanism
- Human and Rat Dipeptidyl Peptidase III: Biochemical and Mass Spectrometric Arguments for Similarities and Differences
- Recombinant Anti-Stefin A Fab Fragment: Sequence Analysis of the Variable Region and Expression in Escherichia coli
- Green Fluorescent Protein Photobleaching: a Model for Protein Damage by Endogenous and Exogenous Singlet Oxygen
- 193 nm Photolysis of Aromatic and Aliphatic Dipeptides in Aqueous Solution: Dependence of Decomposition Quantum Yield on the Amino Acid Sequence
- NADPH:Protochlorophyllide Oxidoreductase Uses the General Import Route into Chloroplasts
- Superoxide Reactivates Nitric Oxide-Inhibited Catalase
- Erratum: The following abstract was unfortunately omitted from the GBM Fall Meeting section of the Biological Chemistry Special Supplement, Vol. 381, September 2000
- Content Index
- Author Index
- Subject Index
Articles in the same Issue
- Circadian Rhythms of Sterol 12α-Hydroxylase, Cholesterol 7α-Hydroxylase and DBP Involved in Rat Cholesterol Catabolism
- Mannosidase Action, Independent of Glucose Trimming, Is Essential for Proteasome-Mediated Degradation of Unassembled Glycosylated Ig Light Chains
- Characterization of a Receptor for Heat Shock Protein 70 on Macrophages and Monocytes
- Yeast Translational Activator Cbs2p: Mitochondrial Targeting and Effect of Overexpression
- Mutational Scanning of a Hairpin Loop in the Tryptophan Synthase β-Subunit Implicated in Allostery and Substrate Channeling
- The Difference in the Carboxy-Terminal Sequence Is Responsible for the Difference in the Activity of Chicken and Rat Liver Fructose-2,6-Bisphosphatase
- Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue
- Primary Structure of Potato Kunitz-Type Serine Proteinase Inhibitor
- Activation of proPHBSP, the Zymogen of a Plasma Hyaluronan Binding Serine Protease, by an Intermolecular Autocatalytic Mechanism
- Human and Rat Dipeptidyl Peptidase III: Biochemical and Mass Spectrometric Arguments for Similarities and Differences
- Recombinant Anti-Stefin A Fab Fragment: Sequence Analysis of the Variable Region and Expression in Escherichia coli
- Green Fluorescent Protein Photobleaching: a Model for Protein Damage by Endogenous and Exogenous Singlet Oxygen
- 193 nm Photolysis of Aromatic and Aliphatic Dipeptides in Aqueous Solution: Dependence of Decomposition Quantum Yield on the Amino Acid Sequence
- NADPH:Protochlorophyllide Oxidoreductase Uses the General Import Route into Chloroplasts
- Superoxide Reactivates Nitric Oxide-Inhibited Catalase
- Erratum: The following abstract was unfortunately omitted from the GBM Fall Meeting section of the Biological Chemistry Special Supplement, Vol. 381, September 2000
- Content Index
- Author Index
- Subject Index
