Noncovalent Binding of Poly(ADP-Ribose) to Nuclear Matrix Proteins: Developmental Changes and Tissue Specificity
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M. Malanga
Abstract
Poly(ADP-ribose) is a nuclear polynucleotide involved in the regulation of chromatin functions via covalent and/or noncovalent modification of nuclear proteins. Using a binding assay on protein blots, we searched for poly(ADP-ribose) binding proteins in nuclear matrices from testes of differently aged rats as well as from various adult rat tissues (brain, liver, spleen). We found that nuclear matrix proteins represent a significant subset of the nuclear proteins that can establish noncovalent interactions with poly(ADP-ribose). The profiles of poly(ADP-ribose) binding nuclear matrix proteins appeared to be tissue-specific and changed during postnatal development in the testis.
The isolation and analysis of endogenous poly (ADP-ribose) from rat testes showed that the ADP-ribose polymers that bind nuclear matrix proteins in vitro are also present under physiologic conditions in vivo. These results further substantiate the possibility that poly(ADP-ribose) may affect chromatin functions through noncovalent interaction with specific protein targets, including nuclear matrix components.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Purification and Cloning of Type A/B hnRNP Proteins Involved in Transcriptional Activation from the Rat spi 2 Gene GAGA Box
- Release of Ribosome-Bound 5S rRNA upon Cleavage of the Phosphodiester Bond between Nucleotides A54 and A55 in 5S rRNA
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- Mutational Analysis of Two PWW Sequence Motifs in Human Aminoacylase 1
- Divergent Enzyme Kinetics and Structural Properties of the Two Human Mitochondrial Creatine Kinase Isoenzymes
- Cloning, Heterologous Expression and Kinetic Analysis of Glycerol Kinase (TbGLK1) from Trypanosoma brucei
- Identification of the 50S Ribosomal Proteins from the Eubacterium Thermus thermophilus
- The Hyper-Thermostable Fe-Superoxide Dismutase from the Archaeon Acidianus ambivalens: Characterization, Recombinant Expression, Crystallization and Effects of Metal Exchange
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- Kinetic and Thermodynamic Analysis of Leech-Derived Tryptase Inhibitor Interaction with Bovine Tryptase and Bovine Trypsin
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- Trichostatin A-Mediated Regulation of Gene Expression and Protein Kinase Activities: Reprogramming Tumor Cells for Ribotoxic Stress-Induced Apoptosis
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