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Association of Betaine-Homocysteine S-Methyltransferase with Microtubules
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Cristinel Sandu
Published/Copyright:
June 1, 2005
Abstract
In mammals, betaine of the mitochondrial matrix is used in the cytosol by betainehomocysteine Smethyltransferase for methionine synthesis. The resulting dimethylglycine is shuttled back into the mitochondrial matrix for further degradation. Nanospray tandem mass spectrometry and Nterminal amino acid sequencing of microtubuleassociated proteins from rat liver tubulin revealed that betainehomocysteine Smethyltransferase is microtubule associated. This was confirmed by confocal laser scanning microscopy of HepG2 cells labeled with betainehomocysteine Smethyltransferase and αtubulinspecific monoclonal antibodies. The association of betainehomocysteine Smethyltransferase with the cytoskeleton may functionally integrate the mitochondrial and cytoplasmic compartments of choline degradation.
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Published Online: 2005-06-01
Published in Print: 2000-07-04
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Characterization of Regulatory Elements in the 5'-Flanking Region of the GM2 Activator Gene
- Transcriptional Activity of GLI1 Is Negatively Regulated by Protein Kinase A
- Peptide Bond Synthesis: Function of the efp Gene Product
- Molecular Cloning, Expression and Purification of Muscle Fructose-1,6-Bisphosphatase from Zaocys dhumnades: the Role of the N-Terminal Sequence in AMP Activation at Alkaline pH
- The pH-Dependent Interaction of Cinnamomin with Lipid Membranes Investigated by Fluorescence Methods
- Nitric Oxide Detection and Visualization in Biological Systems. Applications of the FNOCT Method
- Processing of V-ATPase Subunit B of Mesembryanthemum crystallinum L. Is Mediated in Vitro by a Protease and/or Reactive Oxygen Species
- The Thioredoxin Boxes of Thyroglobulin: Possible Implications for Intermolecular Disulfide Bond Formation in the Follicle Lumen
- New Bivalent Thrombin Inhibitors with N? (Methyl)Arginine at the P1-Position
- Multiple Promoters Direct the Tissue-Specific Expression of Rat Mitochondrial Glycerol-3-Phosphate Dehydrogenase
- Characterization of SNP, a Novel Tissue- and Phase-Specific Nuclear Protein Expressed during the Proliferative Phase in the Oviduct of the Lizard Podarcis sicula Raf.
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- Re-Evaluation of Amino Acid Sequence and Structural Consensus Rules for Cysteine-Nitric Oxide Reactivity
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