Amylase Isoenzymes: Influence of Temperature and Storage of Samples

Chapters in this book

1. Frontmatter I
2. Introduction V
3. CONTENTS VII
4. List of Contributors XIII
5. SECTION I. CHANGING METHODS
6. Improving the Analytical Sensitivity of Enzyme Assays 1
7. Standardization of Enzyme Assays Based on Determinations by Reference Methods 15
8. A Universal Inclusive Reference System for Clinical Enzymology. Proposal: The International Clinical Enzyme Scale 23
9. Amylase Isoenzymes: Influence of Temperature and Storage of Samples 43
10. 4-Nitrophenyl Oligosaccharides as Substrates for a Serum a-Amylase: Selection of Optimum Chain Length 51
11. Reliability of a New Assay for Chymotrypsin Activity in Stool 65
12. A Sensitive Method for Assay of Lipase Activity by Coupling with ß-Oxidation Enzymes of Fatty Acids 73
13. Determination of PseudoCholinesterase Activity by an Enzyme Coupling Method in the Serum of Patients with Liver Disease 79
14. Measurement of Serum Cholinesterase Activity Using a Centrifugal Analyzer 89
15. Enzymatic Determination of Total Bilirubin in Serum 97
16. Assay of Human Mitochondrial Aspartate Aminotransferase by Rocket Immunoelectrophoresis and Autoradiography 109
17. Determination of Cytidine Deaminase in Blood by High Performance Liquid Chromatography 117
18. Fluorimetric Assay of Angiotensin-I-Converting Enzyme in Cerebrospinal Fluid 125
19. SECTION II. DIAGNOSIS
20. Strategies in the Enzymologic Diagnosis of Myocardial Infarction 133
21. How Reliable is the Laboratory Diagnosis of Acute Myocardial Infarction 147
22. Strategies in the Enzymological Diagnosis of Pancreatic Disease 171
23. Pancreatic Cholesterol Ester Hydrolase: Estimation of Its Activity in the Small Bowel Using the Pancreolauryl-Test and Comparison with Pancreatic Exocrine Function 199
24. Assay of Mitochondrial Aspartate Aminotransferase (m-AST) by Immunoprecipitation and Column Chromatography in Sera of Patients with Liver Disease 207
25. Persistent Acid Lipase Activity in a Case of Cholesterol Ester Storage 217
26. Nature and Clinical Significance of Alkaline Phosphatase-Immunoglobulin Complexes 225
27. Biochemical Targets and Indicators of Drug Action 233
28. Strategies in the Enzymologic Diagnosis of Muscular and Connective Tissue Disease 249
29. Carboxypeptidase N: Spectrophotometry Assay and Clinical Significance 289
30. SECTION III. PATHOPHYSIOLOGY, PHARMACOLOGY AND THERAPEUTICS
31. Enzymes in Inflammation: Aspects of Pathophysiology 297
32. Granulocyte Proteinases as Mediators of Unspecific Proteolysis in Inflammation: A Review 305
33. Enzymes in Collagen Synthesis and Degradation 329
34. Proteolytic Enzymes Related to Blood Pressure Regulation 339
35. Urinary Enzyme Pattern in Malignant Hypertension of Salt-Loaded Dahl Rats 353
36. Effect of Indomethacin on Active and Inactive Renin in Sodium-Replete Man at Rest and During Exercise 359
37. The Enzymes of Catecholamine Metabolism in Man: Catechol-O-Methyltransferase, Monoamine Oxidase and Phenol Sulfotransferase 373
38. Enzymatic Conversion of GABA and 2-Pyrrolidone: An Approach to the Biochemistry of Anxiety 399
39. Serum Creatine Kinase Following Thrombolysis in Acute Myocardial Infarction 411
40. Creatine Kinase Isoenzyme Composition in Skeletal Muscle Biopsies Obtained from Marathon Runners 419
41. Urine Enzyme Patterns in the Rat After Damage to Different Parts of the Nephron 429
42. Urinary Enzymes and Isoenzymes of N-Acetyl-ß- D-Glucosaminidase in the Assessment of Nephrotoxicity 435
43. Effect of Parathyroid Hormone and Calcium on Liver and Kidney Enzymes 445
44. Enzymatic Removal of Lipid Hydroperoxides in Rat Liver Mitochondria and Microsomes 453
45. Effect of Mesenteric Lymphatic Obstruction on Small Intestinal Mucosal Enzymes 461
46. SECTION IV. ENZYME HETEROGENEITY
47. Monoclonal Antibodies: New Tools for Biochemical, Immunochemical and Genetic Analyses of Enzymes and Isozymes 469
48. Gene Re-Arrangement and Messenger Processing As Causes of Enzyme Heterogeneity 505
49. Post-Translationally Modified Forms of Enzymes of Diagnostic Importance 517
50. Human Liver Sorbitol Dehydrogenase: Evidence for Two Forms 535
51. Studies of Alanine Aminopeptidase, Dipeptidyl Aminopeptidase I and II of the Human Seminal Fluid and Prostasomes 545
52. SECTION V. VETERINARY
53. Overview of Veterinary Enzyme Pathophysiology in Rural and Urban Practice 567
54. Animal Models of Erythrocyte Glycolytic Pathway Enzyme Deficiencies: Pyruvate Kinase and Cytochrome b₅ Reductase (NADHMethemoglobin Reductase) 583
55. Animal Models of Erythrocyte Pentose Phosphate and Glutathione Detoxification Pathways 597
56. Naturally-Occurring Models of Inborn Errors of Metabolism: Definition of the Enzymatic Defects 605
57. The Utilization of Clinical Enzymology in the Practice of Veterinary Medicine 615
58. Transfer of Gamma-Glutamyl Transferase from Maternal Colostrum to the Serum of Newborn Goats, Foals and Calves 627
59. The Protective Role of Selenium in Gentamicin- Induced Renal Damage in the Rat 635
60. Species Differences in the In Vitro Response of Plasma Angiotensin-Converting Enzyme to Precursors and Active Forms of Converting Enzyme Inhibitors 641
61. Levo-Carnitine in Serum. Heart, Muscle and Tumoral Tissue in Rats with Yoshida Tumor 647
62. Subject Index 653
63. Index of Contributors 665
64. Backmatter 668