The Nature of Specific and Non-Specific Interactions of Detergents with Proteins: Complexing and Unfolding

Chapters in this book

1. Frontmatter I
2. Preface V
3. Section I. General
4. Letter from J. Wyman to the Members of the Symposium 1
5. Concerted Changes in an Allosteric Macromolecule 3
6. Ligand Interactions in Globular Proteins 15
7. Discussion 25
8. Ligand Binding to Enzyme Complexes 27
9. Discussion 41
10. Properties of Polymeric Proteins Bound to Solid Matrices: Subunit Exchange Chromatography 45
11. Discussion 57
12. Contributions of Aromatic Amino Acid Residues to the Optical Activity of Protein-Ligand Complexes 60
13. Discussion 74
14. Section II. Enzymes
15. The Architecture of the Coenzyme Binding Domain in Dehydrogenases as Revealed by X-Ray Structure Analysis 80
16. Discussion 95
17. The Interaction of Glutamate Dehydrogenase with a Coenzyme Analog 99
18. Discussion 109
19. The Interaction of Adenosine Triphosphate and its Analogs with Myosin and the Modification by Actin 111
20. Discussion 128
21. Isolation of a Guanylnucleotide Binding Protein from Pigeon Erythrocyte Membranes 130
22. Discussion 142
23. On the Binding of Isoleucine and Related Amino Acids to Isoleucyl-tRNA Synthetase from Escherichia Coli MRE 600 145
24. Discussion 151
25. The Effect of Small Molecules on the Affinity of Individual Chains in Hemoglobin and on its Cooperativity 153
26. Discussion 165
27. Cooperative Interactions without Symmetry or Subunits 167
28. Discussion 174
29. A Nuclear Magnetic Resonance Study of Activator Binding by Carboxypeptidase A 176
30. Discussion 193
31. Section III. Repressors
32. Lactose Operator Sequences and the Action of Lac Repressor 197
33. Discussion 211
34. The Active Sites of Lac Repressor 215
35. Discussion 229
36. Lac Repressor 232
37. Discussion 239
38. The Use of Suppressed Nonsense Mutations to Generate Altered Lac Repressor Molecules 242
39. Discussion 256
40. Effect of Alterations in Lac Operator DNA on Repressor Binding 257
41. Discussion 271
42. Interaction of Lac Repressor with Non-Specific DNA Binding Sites 274
43. Discussion 289
44. Section IV. Receptors
45. Binding and Functional States of the Cholinergic Receptor Protein from Torpedo Marmorata 295
46. Discussion 308
47. Subunit Structure and Binding Sites of the Acetylcholine Receptor 312
48. Discussion 320
49. Further Characterization of Purified Acetylcholine Receptor and its Incorporation into Phospholipid Vesicles 322
50. Discussion 333
51. Structural and Functional Studies of an Acetylcholine Receptor 334
52. Discussion 358
53. Section V. Antibodies, Drugs and Metabolites
54. Antibody Combining Sites as a Model for Molecular Recognition 364
55. Discussion 377
56. Antibiotic Action on Enzymes Involved in Peptidoglycan Synthesis 380
57. Discussion 391
58. Problems in Studying Protein Binding with Regard to the Interaction of Steroids with a Specific Binding Globulin 393
59. Discussion 404
60. The Interaction of Low-Molecular Compounds with Serum Albumin as Measured by Optical Methods. Circular Dichroism of Bilirubin-Human Serum Albumin Complexes in the Presence of Alcohols in Aqueous Solution 407
61. Discussion 417
62. Section VI. Detergents and Electrolytes
63. The Nature of Specific and Non-Specific Interactions of Detergents with Proteins: Complexing and Unfolding 422
64. Discussion 434
65. Interaction of Detergents with Proteins: Effect of Counter Ions on the System Ferrimyoglobin- Laurylpyridinium Halides in Aqueous Medium 437
66. Discussion 449
67. Influence of Ions on the Kinetics of Cooperative Conformational Changes in Globular Proteins 451
68. Discussion 460
69. Neutral Salt Effects on the Conformational Stability of Biological Macromolecules 462
70. Discussion 479
71. Concluding Remarks 482
72. Index of Contributors 485
73. Subject Index 488
74. Backmatter 497