Chapter 14. Chromatographic techniques
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Prerna Singh
Abstract
Chromatography is one of the most important and versatile analytical techniques that allow the separation of complex mixtures into their respective components and their subsequent quantitative and qualitative analysis. In this technique, two or more components of a mixture to be separated are distributed between two immiscible phases - stationary phase and mobile phase. The basic principle of separation is based on how the solute or components of a mixture equilibrate between these two phases.
This chapter will cover the classification of different chromatographic techniques based on mobile and stationary phases, and will mainly focus on the separation mechanism (adsorption, partition, ion-exchange, and pore-penetration) along with the analytical applications of these chromatographic methods.
Abstract
Chromatography is one of the most important and versatile analytical techniques that allow the separation of complex mixtures into their respective components and their subsequent quantitative and qualitative analysis. In this technique, two or more components of a mixture to be separated are distributed between two immiscible phases - stationary phase and mobile phase. The basic principle of separation is based on how the solute or components of a mixture equilibrate between these two phases.
This chapter will cover the classification of different chromatographic techniques based on mobile and stationary phases, and will mainly focus on the separation mechanism (adsorption, partition, ion-exchange, and pore-penetration) along with the analytical applications of these chromatographic methods.
Chapters in this book
- Frontmatter I
- Preface V
- Contents VII
- List of authors IX
- About the editors XI
- Abbreviations XIII
-
Section I: Analytical chemistry
- Chapter 1. Introduction to analytical chemistry 3
-
Section II: Qualitative and quantitative aspects of analysis
- Chapter 2. Quantitative aspects of analysis 23
- Chapter 3. Data handling in qualitative and quantitative analysis 37
-
Section III: Spectrochemical methods of analysis
- Chapter 4. UV–visible spectroscopy 59
- Chapter 5. Infrared spectroscopy 81
- Chapter 6. Flame atomic absorption and emission spectroscopy 117
-
Section IV: Thermoanalytical Methods
- Chapter 7 Thermogravimetric (TG) and derivative thermogravimetric analysis (DTG) 147
- Chapter 8. Differential thermal analysis 169
-
Section V: Electroanalytical Methods
- Chapter 9. pH Metry 179
- Chapter 10. Conductance 205
- Chapter 11 Electrochemistry 229
-
Section VI: Separation methods and chromatography
- Chapter 12. Separation methods in analysis 263
- Chapter 13 Chromatographic methods 281
- Chapter 14. Chromatographic techniques 293
- Index 323
Chapters in this book
- Frontmatter I
- Preface V
- Contents VII
- List of authors IX
- About the editors XI
- Abbreviations XIII
-
Section I: Analytical chemistry
- Chapter 1. Introduction to analytical chemistry 3
-
Section II: Qualitative and quantitative aspects of analysis
- Chapter 2. Quantitative aspects of analysis 23
- Chapter 3. Data handling in qualitative and quantitative analysis 37
-
Section III: Spectrochemical methods of analysis
- Chapter 4. UV–visible spectroscopy 59
- Chapter 5. Infrared spectroscopy 81
- Chapter 6. Flame atomic absorption and emission spectroscopy 117
-
Section IV: Thermoanalytical Methods
- Chapter 7 Thermogravimetric (TG) and derivative thermogravimetric analysis (DTG) 147
- Chapter 8. Differential thermal analysis 169
-
Section V: Electroanalytical Methods
- Chapter 9. pH Metry 179
- Chapter 10. Conductance 205
- Chapter 11 Electrochemistry 229
-
Section VI: Separation methods and chromatography
- Chapter 12. Separation methods in analysis 263
- Chapter 13 Chromatographic methods 281
- Chapter 14. Chromatographic techniques 293
- Index 323
