6 Aqueous two-phase systems as a tool for bioseparation – emphasis on organic acids
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Dragomir Yankov
Abstract
Aqueous two-phase systems (ATPS) are universally recognized as an excellent alternative to the conventional separation techniques in the biotechnology, because of their undoubted advantages such as mild and biocompatible conditions, high water content, low interfacial tension, ease of process integration and scale up, etc. The formation of ATPS is due to the incompatibility of two polymers in a common solution. Other types of ATPS are formed by polymer/salt, ionic and/or non-ionic surfactants, inorganic salt/short-chain alcohols, and based on room temperature ionic liquids. ATPS are successfully used (even in large scale) for cells, enzyme and protein separation, while their application for recovery of small molecules such as organic acids, antibiotics, alcohols is more complicated as they are usually hydrophilic and tend to distribute evenly between the phases. The purpose of this paper is to overview and summarize the efforts made for the application of different types of ATPS for the separation of organic acids.
Abstract
Aqueous two-phase systems (ATPS) are universally recognized as an excellent alternative to the conventional separation techniques in the biotechnology, because of their undoubted advantages such as mild and biocompatible conditions, high water content, low interfacial tension, ease of process integration and scale up, etc. The formation of ATPS is due to the incompatibility of two polymers in a common solution. Other types of ATPS are formed by polymer/salt, ionic and/or non-ionic surfactants, inorganic salt/short-chain alcohols, and based on room temperature ionic liquids. ATPS are successfully used (even in large scale) for cells, enzyme and protein separation, while their application for recovery of small molecules such as organic acids, antibiotics, alcohols is more complicated as they are usually hydrophilic and tend to distribute evenly between the phases. The purpose of this paper is to overview and summarize the efforts made for the application of different types of ATPS for the separation of organic acids.
Chapters in this book
- Frontmatter I
- Preface V
- Contents VII
- List of Contributing Authors XI
- 1 Chemical engineering methods in downstream processing in biotechnology 1
- 2 Separation of bio-products by liquid–liquid extraction 17
- 3 Extraction and bioprocessing with supercritical fluids 41
- 4 Ion exchange in downstream processing in biotechnology 63
- 5 Electro-membrane separations in biotechnology 79
- 6 Aqueous two-phase systems as a tool for bioseparation – emphasis on organic acids 95
- 7 Ionic liquid-assisted biphasic systems for downstream processing of fermentative enzymes and organic acids 123
- 8 Application of polymer membranes in downstream processes 155
- Index 165
Chapters in this book
- Frontmatter I
- Preface V
- Contents VII
- List of Contributing Authors XI
- 1 Chemical engineering methods in downstream processing in biotechnology 1
- 2 Separation of bio-products by liquid–liquid extraction 17
- 3 Extraction and bioprocessing with supercritical fluids 41
- 4 Ion exchange in downstream processing in biotechnology 63
- 5 Electro-membrane separations in biotechnology 79
- 6 Aqueous two-phase systems as a tool for bioseparation – emphasis on organic acids 95
- 7 Ionic liquid-assisted biphasic systems for downstream processing of fermentative enzymes and organic acids 123
- 8 Application of polymer membranes in downstream processes 155
- Index 165
