3.5 Manipulation of Cell Growth, Metabolism and Product Quality Attributes

Chapters in this book

1. Frontmatter I
2. Preface V
3. Contents VII
4. List of Contributing Authors IX
5. 1. Introduction
6. 1.1 Industrial Use and Perspectives of Animal Cell Culture 1
7. 2. Cell Lines
8. 2.1 Generation of Cell Lines and Biotechnological Applications 11
9. 2.2 CHO History, CHO Evolution and CHO Genomics – an Unsolvable Enigma? 38
10. 2.3 Cell Lines for Vaccine Production 60
11. 2.4 Cell Line Monitoring: Molecular Cytogenetic Characterization 87
12. 2.5 Quality Control Essentials in Human Cell Culture: Cell Line Cross-contamination and Microbiological Infections 102
13. 3. Genetic Engineering of Cells
14. 3.1 Cell Line Evolution and Engineering 115
15. 3.2 Chromosome Rearrangements and Gene Amplification 127
16. 3.3 Synthetic Biology Principles for Engineering Mammalian Designer Cells 144
17. 3.4 Rational Approaches for Transgene Expression: Targeted Integration and Episomal Maintenance 173
18. 3.5 Manipulation of Cell Growth, Metabolism and Product Quality Attributes 216
19. 4. Engineering of the Product
20. 4.1 Control of Biotheraputics Glycosylation 247
21. 4.2 Manufacturing of Complex Biotherapeutic Protein Products: Medical Need and Rational for Monoclonal Antibody Mixtures, Multispecific Formats, and Fc-fusion Proteins 280
22. 5. Basic Aspects of Animal Cell Cultivation
23. 5.1 Physiology and Metabolism of Animal Cells for Production 301
24. 5.2 Functional -Omics for Cell Lines and Processes: The -Omics Technologies on the Example of CHO Cells 326
25. 5.3 Nutrient Media for Cell Culture Technology 368
26. 6. Upstream and Downstream Process Technology
27. 6.1 Bioreactors for Animal Cell Culture 389
28. 6.2 High Cell Density Cultivation Process 427
29. 6.3 Industrial Cell Culture Process Scale-up Strategies and Considerations 455
30. 6.4 Extraction and Purification of Biologics from Cell Culture: Monoclonal Antibody Downstream Processing 489
31. 7. Monitoring and Control of Processes and Products
32. 7.1 Concepts and Technologies for Advanced Process Monitoring and Control 523
33. 7.2 Analytical Techniques and Quality Control of Protein Therapeutics 571
34. 7.3 Process Characterization for Upstream and Downstream Process Development 598
35. 8. Pharmaceutical Aspects of Biologics from Animal Cell Culture Processes
36. 8.1 Spatiotemporally Controlled Delivery of Biopharmaceuticals 649
37. Index 693