A new method for fast extraction and determination of chlorophylls in natural water
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Nianwei Qiu
Abstract
Algae collection and chlorophyll extraction are two troublesome steps in the traditional methods used for the determination of chlorophyll concentration in natural water. A new method was established in this study for fast collection and extraction of chlorophyll. Based on our results, the optimum centrifugation condition for collecting algae was determined as: 5000 g for 15 min at 4 °C, and the optimum dilution ratio of dimethyl sulfoxide: 90% acetone was 1:4. The specific steps were as follows: the algae in water samples were collected by centrifugation at 5000 g at 4 °C for 15 min. The precipitated algae were suspended with 2 mL DMSO. Then the sample was transferred to a 15 mL centrifuge tube, and the tube was incubated at 65 °C for 1–2 h in the dark until the sample turned white. After cooling, the chlorophyll extract was diluted with 8 mL 90% acetone, before centrifugation at 5000 g for 5 min. The absorbance values of the supernatants at 750, 664, 647 and 630 nm were used for the calculation of chlorophyll concentrations by the trichromatic equations. This new method saved the filter cost, simplified the extraction process, improved the algae acquisition efficiency, and accelerated the chlorophyll extraction rate.
Acknowledgments
This work was supported by the National Natural Science Foundation of China (31471884), the Natural Science Foundation of Shandong Province (ZR2015JL012, ZR2015DL009), the Postgraduate Education Innovation Project of Shandong Province (SDYY16087), the China’s Post-doctoral Science Fund (Grant No. 2016M592158) and the Refined Experimental Project of Qufu Normal University (JP2014006).
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Articles in the same Issue
- Frontmatter
- Three new coumarin types from aerial parts of Ammi majus L. and their cytotoxic activity
- Sesquiterpenes from the Saudi Red Sea: Litophyton arboreum with their cytotoxic and antimicrobial activities
- Biotechnology for bioenergy dedicated trees: meeting future energy demands
- Malusides, novel glucosylceramides isolated from apple pomace (Malus domestica)
- GC-MS characterization of n-hexane soluble fraction from dandelion (Taraxacum officinale Weber ex F.H. Wigg.) aerial parts and its antioxidant and antimicrobial properties
- N-Acetylborrelidin B: a new bioactive metabolite from Streptomyces mutabilis sp. MII
- Synthesis of methyl 4-dihydrotrisporate B and methyl trisporate B, morphogenetic factors of Zygomycetes fungi
- Volatiles from Cinnamomum cassia buds
- A new method for fast extraction and determination of chlorophylls in natural water
- Synthesis and biological evaluation of acyl derivatives of hydroxyflavones as potent antiproliferative agents against drug resistance cell lines
