Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and syringyl alcohol via several plant peroxidases involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis
Abstract
The mechanism of lignin dehydrogenative polymerization (DHP), made by means of horseradish peroxidase (HRP), was studied in comparison with other plant peroxidases. Interestingly, HRP is efficient for guaiacyl type polymer formation (G-DHPs), but is not efficient in the case of syringyl type DHPs (S-DHPs). It was previously demonstrated that lignification-related Arabidopsisthaliana peroxidases, AtPrx2, AtPrx25 and AtPrx71, and cationic cell-wall-bound peroxidase (CWPO-C) from Populus alba are successful to oxidize syringyl- and guaiacyl-type monomers and larger lignin-like molecules. This is the reason why in the present study the DHP formation by means of these recombinant peroxidases was tested, and all these enzymes were successful for formation of both G-DHP and S-DHP in acceptable yields. CWPO-C led to S-DHP molecular size distribution similar to that of isolated lignins.
Acknowledgments
This work was supported by the Japan Society for the Promotion of Science (JSPS), Funder ID: 10.13039/501100001691. KAKENHI Scientific Research (B) Grant Number JP17H03846 (Y.T.) and JSPS KAKENHI Young Scientists (B) Grant Number JP15K18724 (J.S.) and Scientific Research (C) Grant Number JP17K07878 (J.S.). We thank Edanz Group (www.edanzediting.com/ac) for editing a draft of the manuscript.
Author contributions: Conceived and designed the experiments: JS and YT. Performed the experiments: JS and HH. Analyzed the data: JS, HH, KF and YT. Contributed to the writing of the manuscript: JS and YT. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Original Articles
- A novel norneolignan glycoside and four new phenolic glycosides from the stems of Viburnum fordiae Hance
- Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and syringyl alcohol via several plant peroxidases involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis
- Simple production of medium density fiberboards (MDF) reinforced with chitosan
- Scrimber board (SB) manufacturing by a new method and characterization of SB’s mechanical properties and dimensional stability
- Improvement of beech wood properties by in situ formation of polyesters of citric and tartaric acid in combination with glycerol
- Effect of the penetration of isocyanates (pMDI) on the nanomechanics of wood cell wall evaluated by AFM-IR and nanoindentation (NI)
- Characteristics of wood-silica composites influenced by the pH value of silica sols
- Dependence of Poisson’s ratio and Young’s modulus on microfibril angle (MFA) in wood
- The influence of bamboo fiber content on the non-isothermal crystallization kinetics of bamboo fiber-reinforced polypropylene composites (BPCs)
- Complete plastid genome sequences of three tropical Alseodaphne trees in the family Lauraceae
Articles in the same Issue
- Frontmatter
- Original Articles
- A novel norneolignan glycoside and four new phenolic glycosides from the stems of Viburnum fordiae Hance
- Generation of lignin polymer models via dehydrogenative polymerization of coniferyl alcohol and syringyl alcohol via several plant peroxidases involved in lignification and analysis of the resulting DHPs by MALDI-TOF analysis
- Simple production of medium density fiberboards (MDF) reinforced with chitosan
- Scrimber board (SB) manufacturing by a new method and characterization of SB’s mechanical properties and dimensional stability
- Improvement of beech wood properties by in situ formation of polyesters of citric and tartaric acid in combination with glycerol
- Effect of the penetration of isocyanates (pMDI) on the nanomechanics of wood cell wall evaluated by AFM-IR and nanoindentation (NI)
- Characteristics of wood-silica composites influenced by the pH value of silica sols
- Dependence of Poisson’s ratio and Young’s modulus on microfibril angle (MFA) in wood
- The influence of bamboo fiber content on the non-isothermal crystallization kinetics of bamboo fiber-reinforced polypropylene composites (BPCs)
- Complete plastid genome sequences of three tropical Alseodaphne trees in the family Lauraceae