Time-resolved fluorescence methods (IUPAC Technical Report)
Helge Lemmetyinen, et al. Pure and Applied Chemistry, 2014, Volume 86, Issue 12, pp. 1969-1998
This report describes and compares the currently applied methods for measuring and analyzing time-resolved fluorescence traces using phase-modulation fluorometry as well as pulse fluorometry (direct emission decay measurements, single-photon timing, streak camera measurements, fluorescence upconversion, and optical Kerr gating). The paper starts with a brief description of the basic principles for time and frequency domain fluorescence spectroscopy. The fundamental equations are given, and recommendations for adequate use are emphasized. The up-to-date, commonly-employed excitation sources and photodetectors are described in detail. The analysis of time-resolved fluorescence data is discussed. Attention is paid to possible artifacts and remedies are presented on how to avoid them or to account for them. Finally, fluorescence lifetime standards for the nanosecond and picosecond timescales are collected. This report was prepared in the framework of IUPAC project 2004-021-1-300.
©2015 by Walter de Gruyter Berlin/Boston
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Articles in the same Issue
- Masthead - Full issue pdf
- Guest Editorial
- Secretary General's Column
- Secretariat on the Move
- Features
- Taking IUPAC Literally: An International Union of Pure and Applied Chemistry
- Twitter in Chemical Education, and IUPAC
- Drug Design and Development: Part II: Reflections from an Academic-based Center
- Youth Views on Sustainability: PMMA, from plexiglass window to a packaging for an implantable glucose sensor
- IUPAC Wire
- Launch event for SDGs review held at UN in New York
- African Leaders Endorse a Science Funding Platform for Africa: the Alliance for Accelerating Excellence in Science in Africa (AESA)
- The EuCheMS Award for Service - Call for Nominations
- In Memoriam: Anthony R. H. Goodwin
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- Election of IUPAC Officers and Bureau Members: Call for Nominations
- Project Place
- Increasing IUPAC's Social Media Presence
- IUPAC Provisional Recommendations
- Glossary of Terms Used in Neurotoxicology
- Making an imPACt
- Nomenclature and graphic representations for chemically modified polymers (IUPAC Recommendations 2014)
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