Evaluation of red blood cell parameters provided by the UF-5000 urine auto-analyzer in patients with glomerulonephritis

Genki Mizuno; Masato Hoshi; Kentaro Nakamoto; Masayo Sakurai; Kazuko Nagashima; Takashi Fujita; Hiroyasu Ito; Tadayoshi Hata

doi:10.1515/cclm-2021-0287

Article

Evaluation of red blood cell parameters provided by the UF-5000 urine auto-analyzer in patients with glomerulonephritis

Genki Mizuno , Masato Hoshi , Kentaro Nakamoto , Masayo Sakurai , Kazuko Nagashima , Takashi Fujita , Hiroyasu Ito and Tadayoshi Hata

Published/Copyright: April 28, 2021

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From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 59 Issue 9

Abstract

Objectives

The microscopic examination of hematuria, a cardinal symptom of glomerulonephritis (GN), is time-consuming and labor-intensive. As an alternative, the fully automated urine particle analyzer UF-5000 can interpret the morphological information of the glomerular red blood cells (RBCs) using parameters such as UF-5000 small RBCs (UF-%sRBCs) and Lysed-RBCs.

Methods

Hematuria samples from 203 patients were analyzed using the UF-5000 and blood and urine chemistries to determine the cut-off values of RBC parameters for GN and non-glomerulonephritis (NGN) classification and confirm their sensitivity to the IgA nephropathy and non-IgA nephropathy groups.

Results

The UF-%sRBCs and Lysed-RBCs values differed significantly between the GN and NGN groups. The cut-off value of UF-%sRBCs was >56.8% (area under the curve, 0.649; sensitivity, 94.1%; specificity, 38.1%; positive predictive value, 68.3%; and negative predictive value, 82.1%), while that for Lysed-RBC was >4.6/μL (area under the curve, 0.708; sensitivity, 82.4%; specificity, 56.0%; positive predictive value, 72.6%; and negative predictive value, 69.1%). Moreover, there was no significant difference in the sensitivity between the IgA nephropathy and non-IgA nephropathy groups (87.1 and 89.8% for UF-%sRBCs and 83.9 and 78.4% for Lysed-RBCs, respectively). In the NGN group, the cut-off values showed low sensitivity (56.0% for UF-%sRBCs and 44.0% for Lysed-RBCs).

Conclusions

The RBC parameters of the UF-5000, specifically UF-%sRBCs and Lysed-RBCs, showed good cut-off values for the diagnosis of GN.

Keywords: glomerulonephritis; hematuria; Lysed-RBC; small red blood cell; UF-5000

Corresponding author: Masato Hoshi, PhD, Department of Biochemical and Analytical Science, Fujita Health University, 1-98 Dengakugakubo, Kutsukakecho, Toyoake, Aichi 470-1192, Japan, Phone: +81 562 93 2532, Fax: +81 562 93 2519, E-mail: mhoshi@fujita-hu.ac.jp

Funding source: Fujita Health University Grant

Acknowledgments

We would like to thank Editage (www.editage.jp) for English language editing.

Research funding: This study was supported by a Fujita Health University Grant (M. H.).
Author contributions: Mizuno G and Hoshi M planned the study. Mizuno G, Hoshi M, Nakamoto K, Sakurai M, Nagashima K, and Fujita T performed the experiments. Mizuno G, Hoshi M, and Nakamoto K were responsible for the data integrity and analysis. Mizuno G, Hoshi M, Nakamoto K, Sakurai M, Nagashima K, Fujita T, Ito H, and Hata T discussed the results. Mizuno G, Hoshi M, and Nakamoto K wrote the manuscript. Hoshi M, Ito H, and Hata T conducted the study. Hata T assumes the primary responsibility for the final content. All authors reviewed the manuscript.
Competing interests: The authors declare no potential conflicts of interest.
Informed consent: Informed consent was obtained from patients through an opt-out form posted on the hospital wall.
Ethical approval: Research involving human subjects complied with all relevant national regulations, institutional policies and is in accordance with the tenets of the Helsinki Declaration (as revised in 2013), and has been approved by the authors’ Institutional Review Board Ethics Review Committee of Fujita Health University or equivalent committee (approval no. HM19-182).

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2021-0287).

Received: 2021-03-09

Accepted: 2021-04-20

Published Online: 2021-04-28

Published in Print: 2021-08-26

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Articles in the same Issue

https://doi.org/10.1515/cclm-2021-0287

Keywords for this article

glomerulonephritis; hematuria; Lysed-RBC; small red blood cell; UF-5000