Band 9, Heft 5

Age-related macular degeneration (AMD) is a neurodegenerative disease associated with irreversible loss of central vision in the elderly. Disruption of the homeostatic function of the retinal pigment epithelium (RPE) is thought to be fundamental to AMD pathogenesis, and oxidative stress is implicated in the associated RPE damage. We examined the effects of oxidative stress on the expression of junctional proteins in cultured human retinal pigment epithelial (ARPE-19) cells. Reverse transcription-PCR and immunoblot analyses revealed that expression of the tight-junction protein claudin-1 was increased at both the mRNA and protein levels 8 to 12 h after exposure of ARPE-19 cells to H2O2, whereas that of the tight-junction protein ZO-1 or the adherens-junction protein N-cadherin was unaffected. Expression of both claudin-1 and N-cadherin was down-regulated by exposure of the cells to H2O2 for longer periods (24 to 48 h). Oxidative stress also induced the phosphorylation of p38 mitogen-activated protein kinase (MAPK) with a time course similar to that apparent for the up-regulation of claudin-1 expression. Furthermore, the increase in the abundance of claudin-1 induced by H2O2 was blocked by the p38 inhibitor SB203580. Phosphorylation of the MAPKs ERK and JNK was not affected by H2O2. Our results suggest that modulation of claudin-1 expression in the RPE by oxidative stress may contribute to the pathogenesis of AMD.

AMD is the main cause of visual impairment in people over 50 years of age and the most common cause of blindness. In recent years, the use of bevacizumab to treat neovascular AMD has become a preferred treatment in the United States. However, whether bevacozumab is available for RPE or AMD patients is unknown. We firstly indicate that Pam3CSK4 (P3C) activates TLR2 pathway during ARPE-19 apoptosis as determined by western blotting. And then, the expression of MyD88, NF-κB, p-IKK in primary RPE cells from AMD patients is significantly down-regulated after treatment with 50 µg L−1 Bevacizumab. Therefore, our data shows that MyD88 is involved in the TLR2 pathway in ARPE-19 cell apoptosis resulting from Pam3CSK4 (P3C). And more importantly, our findings suggested that Bevacizumab cured age-related macular degeneration (AMD) via down-regulate Toll—like receptor 2 (TLR2) pathway in RPE from AMD patients.

Adult body size is one of the most ecologically relevant quantitative traits that underlies many other life-history traits of particular organism. In insects, there is positive intraspecific relationship between body size and female fecundity. In this study small scale temporal and spatial and space variability in structural body size of Poecilus cupreus and Anchomenus dorsalis was investigated. The beetles were collected in four fields near Prague-Suchdol in autumn 2009 and 2010, and in spring 2010, 2011 and 2012. In both species structural body size was significantly affected by sex (females were the larger sex). In A. dorsalis structural body size was also significantly affected by arable field identity, overwintering (post-overwintering individuals collected in spring were larger in comparison to pre-overwintering individuals collected in autumn), sampling year, overwintering by year and arable field by year interactions. Our results suggest that spatiotemporal variation in environmental conditions experienced by A. dorsalis during larval growth resulted in differences in adult structural body size among particular fields and particular sampling years. In addition, mean structural body size in A. dorsalis was affected by overwintering, which was probably caused by size-specific winter mortality. Moreover, effect of overwintering varied among years, probably according to the specific weather conditions during a particular winter.

Using vegetation databases from Poland, the Czech Republic, Austria, Slovakia and our own material collected during a field work, we have analysed the variability of species composition of Abies alba-dominated communities in Central Europe. Analysis was performed using a modified TWINSPAN algorithm in the JUICE software. Ecological analysis was performed on the basis of mean Ellenberg indicator values with a MoPeT_v1.0.r script prepared in R software. In general, the findings indicated that there are at least 8 types of Central European silver fir-dominated forests with different patterns in species composition and habitat conditions. These are the oligotrophic subcontinental silver fir forests of the Polish highlands, oligotrophic (sub)montane silver fir forest, hygrophilous silver fir forests, oligo-mesotrophic (sub)montane silver fir forests, mesotrophic (sub)montane silver fir forests, submontane and montane calcicolous silver fir forests of the Alps and the Carpathians, eutrophic silver fir-beech forests and silver fir ravine forests. The main conclusion is that the diversity of species composition of Abies alba-dominated forests in Central Europe is lower than described in current regional synthesis, which was confirmed by ecological analyses.

The assessment of heat and drought tolerance is of primary importance in breeding programmes designed to improve heat and drought tolerance in cereals. Three winter wheat varieties grown in controlled growth chambers were exposed to heat (H) and drought (D) stress singly and in combination (H+D). The combined effects of H and D stress were much more severe than those of individual treatments for both physiological and yield parameters during grain filling. The chlorophyll content, effective quantum yield of PSII, net assimilation rate, transpiration, stomatal conductance and intercellular CO2 concentration were greatly reduced by H, D and their interaction. Grain yield decreased to a greater extent (48.3%) in Plainsman V, averaged over the stress treatments, than in Mv Magma (67.8%) and Fatima 2 (53.7%). The least decline was found in grain number, except in Plainsman V. Mv Magma tolerated heat stress better than Fatima 2. In terms of photosynthetic activity, Plainsman V showed better drought tolerance than Mv Magma. The results showed that changes in physiological properties during stress treatment are not always associated with changes in yield parameters, so a combination of methods may be needed to give a more precise picture of the stress tolerance of wheat varieties.

Infectious bursal disease (IBD) caused by non-enveloped double stranded RNA virus is an acute and contagious poultry disease. Outbreak of IBD could result in 10–75% mortality of the birds; hence it has gained socio-economic importance worldwide. Medicinal plants have shown broad spectrum anti-viral activities against RNA and DNA viruses. Moringa oleifera Lam (MOL), Phyllanthus emblicus Linn (PEL), Glycyrrhiza glabra Linn (GGL), and Eugenia jambolana Lam (EJL) are commonly available medicinal plants of the sub-continent and exhibited anti-viral potential against different viruses. Ethanolic extracts of the leaves of MOL and EJL, roots of GGL and dried fruit of PEL were investigated for their cytotoxic and anti-viral potential against IBD virus using MTT colorimetric assay and anti-viral assay. Significant anti-viral potential (P<0.001) was demonstrated at concentrations 12.5, 25, 50 and 100 µg ml−1 of GGL, PEL, MOL and EJL, respectively, with no cytotoxicity. Data also spotlighted that all tested plant extracts possess significant anti-viral potential and this trend was higher in GGL followed by PEL, MOL, and EJL. The data undoubtedly conclude that these medicinal plants contain several health beneficial phyto-chemicals which got significant anti-viral potential and effectively be utilized against IBD virus. Moreover, the outcomes of this study provide a platform on the way to discover novel anti-viral agents against IBD virus and other viruses from plant origin.

The European tarnished plant bug (Lygus rugulipennis Poppius) is among the most serious pests in the family Miridae, and therefore there is increasing interest in understanding the behaviour of this species. In the present study, laboratory recordings were taken using a laser vibrometer on adult males and females to ascertain whether acoustic signals are involved in intraspecific communication. Recordings were both carried out on plant and loudspeaker membrane substrates. Males and females emitted vibratory signals and the present results indicate that these signals are important during courtship. The basic signal characteristics measured were the dominant frequency, pulse duration, repetition time and number of pulses per group within the signal. Male and female signals did not differ in respect to any of these characteristics. Plant recorded signals were longer because of different mechanical properties of substrates. Additionally, the high frequency components were attenuated due to the low-pass filtering properties of plants. As this is the first study on vibratory communication of the European tarnished plant bug, we believe these findings may contribute considerably to the better understanding of the mating behavior of this important pest species.

This study investigated the toxic effects of 12 substituted benzenes exposed to Propsilocerus akamusi larvae singly and as mixtures. Their toxicities were quantified in terms of median effective concentration (EC50) killing 50% of the larvae. For individual substituted benzenes to 4th-instar P. akamusi larvae, the toxicity was in decreasing order of p-chlorophenol > nitrobenzene > phenol > 1,2-dimethylbenzene > 1,3-dimethylbenzene > chlorobenzene > p-phenylenediamine > 1,4-dimethylbenzene > m-phenylenediamine > methylbenzene > benzene > aniline. The order of toxicity among three isomers of dimethylbenzene was 1,2-dimethylbenzene > 1,3-dimethylbenzene > 1,4-dimethylbenzene while p-phenylenediamine > m-phenylenediamine. The binary substituted benzene compounds’ toxicities were evaluated by toxic unit (TU), additive index (AI), mixture toxicity index (MTI) and similarity parameter index (λ). The evaluation results of TU and MTI for 9 substituted benzene compounds were completely consistent while the results of AI were the same as the results of λ based on 24 h EC50 of binary substituted benzenes. The evaluation results of 10 substituted benzene compounds were consistent using TU, MTI, AI and λ evaluation methods. 52.63% and 47.37% of binary substituted benzene tests on P. akamusi larvae showed synergism and partial addition/antagonism, respectively, under mixtures of equal proportions. These results suggest that substituted benzenes indicate acute and binary joint toxicity to P. akamusi.

Micromeria longipedunculata Bräuchler (syn. M. parviflora (Vis.) Rchb.) is an endemic Illyric-Balkan plant species from Bosnia and Herzegovina, Montenegro, and Albania. We investigated types and distribution of trichomes, pollen morphology, and chemical composition of essential oil (analysed by GC and GC-MS) in M. longipedunculata. Non-glandular trichomes, peltate trichomes, and two types of capitate trichomes (type 1 composed of one basal epidermal cell, and one head cell with subcuticular space; type 2 composed of one basal epidermal cell, two or three stalk cells, and one head cell with subcuticular space) were observed on leaves, bracteoles, the calyx, corolla, and the stem. The pollen grains had six apertures which were set in the equatorial pollen belt and showed medium reticulate ornamentation. A phytochemical analysis of essential oils from four different localities is characterized by similar chemical composition with spathulenol (23.7–39.5%), piperitone oxide (7.7–12.1%) and piperitone (7.3–8.9%) as the major compounds.

Rhamnus alaternus and R. ludovici-salvatoris, two Mediterranean shrubs with different geographic distributions, have shown important differences in seedling recruitment capacity. The objectives of this work were to determine the ability of these species to germinate seeds under different temperature ranges, as well as the capacity of seedlings to emerge from different burial depths, in order to better understand their regeneration processes. Two different experiments were performed. In the first one, seed germination was studied in Petri dishes and in the dark at different temperature regimes: a) 5–15°C, b) 10–20°C and c) 15–25°C (12h/12h). In the second experiment, seedling emergence capacity from different burial depths (0.5, 2 and 5 cm) was tested. R. ludovici-salvatoris showed a significantly higher final germination rates, a lower dormancy period, and average time response at 10–20°C than at other temperature ranges, although differences were much greater when seeds were subjected to the 5–15°C temperature regime. By contrast, R. alaternus did not show significant differences between treatments (5–15°C and 10–20°C) in germination behavior. Seedling emergence of both species was lower and slower when seeds were buried at 5 cm. However, R. ludovici-salvatoris always showed a lower seedling emergence capacity than R. alaternus at any burial depth. The low ability of R. ludovici-salvatoris to germinate seeds and emerge between 5–15°C, even from shallow depths, is discussed in relation to its low regeneration capacity and declining geographic distribution.

This study investigates the genetic and morphological variability of the lichen Cladonia rei inhabiting strongly contaminated postsmelting slag dumps in southern Poland. Altogether, 27 C. rei samples were analysed, including 17 from a single population in one dump. The phylogenetic analysis includes samples of C. rei, outgroup species, and external sequences of Cladonia section representatives from GenBank. Comparative analysis of the internal transcribed spacer (ITS) rDNA sequences revealed the presence of 19 C. rei haplotypes overall, including several of the most frequent, of which 11 are represented by single individuals only. As many as 12 haplotypes were recorded within a single population. Three strongly supported monophyletic clades comprised of specimens from different geographical regions were recovered. Morphometric analysis showed great phenotypic variability within particular clades. Apart from a full range of previously known morphological forms of the species, an additional specific morphotype was recognised in the dumps; however, its representatives do not create a monophyletic group. High genetic variability within a single population suggests that C. rei has a great potential for colonising anthropogenic habitats. This attribute emphasises the role of this lichen as an essential pioneer in the early stages of natural regeneration of such sites.