In vitro Investigation of Anticancer, Cell-Cycle-Inhibitory, and Apoptosis-Inducing Effects of Diversin, a Natural Prenylated Coumarin, on Bladder Carcinoma Cells
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Azadeh Haghighitalab
,
Ahmad Reza Bahrami
Chemotherapy is one of the main strategies for reducing the rate of cancer progression or, in some cases, curing the tumour. Since a great number of chemotherapeutic agents are cytotoxic compounds, i. e. similarly affect normal and neoplastic cells, application of antitumour drugs is preferred in cancer management and therapy. In this study, the cytotoxicity of diversin was evaluated in 5637 cells, a transitional cell carcinoma (TCC) subline (bladder carcinoma), and normal human fibroblast cells using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Chromatin condensation and DNA damage induced by diversin were also determined by means of 4’,6-diamidino-2- phenylindole (DAPI) staining and the comet assay, respectively. In addition, the mechanism of action of diversin was studied in more detail by the caspase 3 colourimetric assay and flow cytometry-based cell-cycle analyses (PI staining). Our results revealed that diversin has considerable cytotoxic effects in 5637 cells, but not on HFF3 (human foreskin fibroblast) and HDF1 (human dermal fibroblast) cells. Further studies showed that diversin exerts its cytotoxicity via induction of chromatin condensation, DNA damage, and activation of caspase 3 in 5637 cells. In addition, flow cytometric analyses revealed that 5637 cells are mostly arrested at the G2 phase of the cell cycle in the presence of diversin.
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Articles in the same Issue
- A New Ergostane Triterpenoid from a Solid Culture of the Basidiomycete Inocybe lilacina
- Non-Alkaloidal Compounds from the Bulbs of the Egyptian Plant Pancratium maritimum
- In vitro Investigation of Anticancer, Cell-Cycle-Inhibitory, and Apoptosis-Inducing Effects of Diversin, a Natural Prenylated Coumarin, on Bladder Carcinoma Cells
- Antileishmanial Compounds from Moringa oleifera Lam.
- Cytotoxic Polyacetylenes from the Red Sea Sponge Siphonochalina siphonella
- The Co-Occurrence of Two Pyridine Alkaloids, Mimosine and Trigonelline, in Leucaena leucocephala
- Aqueous Garlic Extract Alleviates Liver Fibrosis and Renal Dysfunction in Bile-Duct-Ligated Rats
- The Wheat Aquaporin Gene TaAQP7 Confers Tolerance to Cold Stress in Transgenic Tobacco
- Isolation and Characterization of Wild-Type Lipoxygenase LOXPsa1 from Pleurotus sapidus
- Molecular Cloning and Characterization of a Bombyx mori Gene Encoding the Transcription Factor Atonal
- Total Antioxidant Capacity of Feces of Mammalian Herbivores and Carnivores
- Direct Asymmetric Aldol Reactions Catalyzed by Lipase from Porcine Pancreas
Articles in the same Issue
- A New Ergostane Triterpenoid from a Solid Culture of the Basidiomycete Inocybe lilacina
- Non-Alkaloidal Compounds from the Bulbs of the Egyptian Plant Pancratium maritimum
- In vitro Investigation of Anticancer, Cell-Cycle-Inhibitory, and Apoptosis-Inducing Effects of Diversin, a Natural Prenylated Coumarin, on Bladder Carcinoma Cells
- Antileishmanial Compounds from Moringa oleifera Lam.
- Cytotoxic Polyacetylenes from the Red Sea Sponge Siphonochalina siphonella
- The Co-Occurrence of Two Pyridine Alkaloids, Mimosine and Trigonelline, in Leucaena leucocephala
- Aqueous Garlic Extract Alleviates Liver Fibrosis and Renal Dysfunction in Bile-Duct-Ligated Rats
- The Wheat Aquaporin Gene TaAQP7 Confers Tolerance to Cold Stress in Transgenic Tobacco
- Isolation and Characterization of Wild-Type Lipoxygenase LOXPsa1 from Pleurotus sapidus
- Molecular Cloning and Characterization of a Bombyx mori Gene Encoding the Transcription Factor Atonal
- Total Antioxidant Capacity of Feces of Mammalian Herbivores and Carnivores
- Direct Asymmetric Aldol Reactions Catalyzed by Lipase from Porcine Pancreas
