A Comparative Study of Sensitivity of Acetylcholinesterase in Detection of Organophosphorus Pesticide Residues
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Sun Xia
In this paper, four enzymes from different sources used for pesticide detection were tested and compared: homogenate from chicken liver, chicken brain and cyprinoids brain were made respectively in the laboratory and Acetylcholinesterase (AChE) was purchased from Musca domestica. The inhibition of AChE activity under different concentrations of omethoate, trichlorfon and dichlorvos were compared. The results showed that the AChE activity in chicken liver and cyprinoids brain were very low. The capacities of activity inhibition of other two kinds of AChE were better. Chicken brain-esterase's inhibition rate achieved 24.16%, 50.56%, and 54.1% for 100ug/L of omethoate, trichlorfon and dichlorvos respectively. Chicken brain-esterase has high specific activity, is low cost, and easy to prepare, and the inhibition of the sensitivity to three organophosphorus pesticides is significantly higher than enzymes from chicken liver and cyprinoids brain. So the chicken brain-esterase could be a good choice to use to detect pesticide residues in agricultural products.
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Articles in the same Issue
- Article
- Analysis of Dough Sheeting and Heat Transfer during Baking of Unleavened Flat Bread (Chapathi)
- Ohmic Heating Behavior of Certain Selected Liquid Food Materials
- Mathematical Models for Prediction of Rheological Parameters of Pineapple Juice
- Comparative Study of Total Phenol, Flavonoid Contents and Antioxidant Activity in Canna indica and Hibiscus rosa sinensis: Prospective Natural Food Dyes
- Modeling Drying Kinetics of Mustard in Fluidized Bed
- A Comparative Study of Sensitivity of Acetylcholinesterase in Detection of Organophosphorus Pesticide Residues
- Development of a Microwave Treatment Technique for Bacterial Decontamination of Raw Meat
- Modelling and Simulation Moisture Transfer in Wheat Stored in a Simulated Sealed Pit
- Different Strategies of Far-Infrared Radiation Application in Paddy Drying
- Producing Powders of Hibiscus Extract in a Laboratory-Scale Spray Dryer
- Shorter Communication
- Modeling of Oil Palm Fruit Maturity for the Development of an Outdoor Vision System
