Media Optimization for the Production of ?- Linolenic Acid by Cunninghamella echinulata var.elegans MTCC 552 Using Response Surface Methodology
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Amit Shrivastava
?- Linolenic acid (GLA) is an intracellular primary metabolite produced by some plants and fungal species via several distinct biosynthetic pathways. It is an essential fatty acid, and is mainly used in treatment of various diseases such as rheumatoid arthritis, multiple sclerosis, schizophrenia, atopic eczema and premenstrual syndrome. It is also a precursor of a number of biologically active compounds. In present work, strains of Cunninghamella echinulata var.elegans MTCC 552, Mucor rouxii MTCC 386, Mucor hiemallis MTCC 1278 and Rhizopus stolnifer MTCC 2591 were screened for maximum production of GLA. Cunninghamella echinulata var. elegans MTCC 552 produced maximum GLA (13.7 ± 0.31 mg/g of DCW), and hence selected for further work. Many crucial factors that affect GLA production were optimized in two steps. In the first step, one factor at a time method was used to investigate the effects of media constituents. Subsequently, in the second step, concentration of media components was optimized using Response Surface Methodology (RSM). These optimizations increased the GLA production to 19.8 ± 0.35 mg/g of DCW.
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Articles in the same Issue
- Article
- Optimization of Osmotic Dehydration Process of Carrot Cubes in Sodium Chloride Solution
- Fatty Acid Composition of Turkey Meat Sucuk (Soudjuck), Salami, and Sausages
- Simulation of an Effect of a Baffle Length on the Power Consumption in an Agitated Vessel
- Equilibrium Moisture Content Models for Lafun
- Optimisation of Cassava Pellet Processing Method
- Numerical Simulation of the Water Diffusion in Cylindrical Solids
- Thin Layer Drying Characteristics of Eriste: A Dried Cereal Product of Turkey
- Effect of Extraction Temperature on Functional Properties of Rice Bran Protein Concentrates
- Experimental Studies on Pressure Control Using Intelligent PI Fuzzy Supervisory Approach
- Media Optimization for the Production of ?- Linolenic Acid by Cunninghamella echinulata var.elegans MTCC 552 Using Response Surface Methodology
- Increasing Shelf Life of Strawberries Using an Irradiation-Cooling Process
- Combined Radiant and Conductive Vacuum Drying in a Vibrated Bed
- Enzyme Hydrolysis of Silk Fibroin and the Anti-diabetic Activity of the Hydrolysates
- Discrimination of Wines Produced from Cabernet Sauvignon Grapes Treated with Aqueous Ethanol Post-Bloom Using an Electronic Nose
- Air-impingement De-shelling of Chestnuts (C. mollisima): Process Parameter Optimization
