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In-Situ-ATR-FTIR Detection of Protein Sorption at Polyelectrolyte Multilayers: Variation of the Thickness
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M. Müller
Published/Copyright:
September 25, 2009
In-situ ATR FTIR spectroscopy was used to study protein adsorption at polyelectrolyte multilayers (PEM). PEM were fabricated by consecutively adsorbing poly(ethyleneimine) (PEI) and poly(acrylic acid) (PAC) analogously to the technique introduced by Decher at silicon internal reflection elements in an in-situ ATR cell. The thickness of PEM of PEI/PAC could be varied by pH value, concentration and number of adsorption steps. At this PEM system adsorption of human serum albumin was studied under electrostatically attractive conditions in dependence of the thickness. Evidence was found, that HSA was predominantly bound in the uncompensated outermost layer region rather than in the bulk phase of the PEM.
Received: 2006-4-24
Accepted: 2006-7-27
Published Online: 2009-9-25
Published in Print: 2007-1-1
© Oldenbourg Wissenschaftsverlag
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Articles in the same Issue
- Bunsen Colloquium: Controlling Protein Adsorption at Materials Surfaces
- Adsorption of Collagen Fragments on Titanium Oxide Surfaces: A Molecular Dynamics Study
- Adsorption of the Flexible Salivary Proteins Statherin and PRP-1 to Negatively Charged Surfaces – A Monte Carlo Simulation and Ellipsometric Study
- Surface-Tethered Polymers to Influence Protein Adsorption and Microbial Adhesion
- Lactoperoxidase and Histatin 5 – their Adsorption Behaviour on Silica and Hydrophobized Silica Surfaces, and Implications on their Role in the Initial Salivary Film Formation
- Ultrasensitive Fluorescence Microscopy Studies of Protein Interactions with Functionalized Surfaces
- Adsorption of Amyloid β (1-40) Peptide at Liquid Interfaces
- Controlled Release of Proteins Bound to Spherical Polyelectrolyte Brushes
- In-Situ-ATR-FTIR Detection of Protein Sorption at Polyelectrolyte Multilayers: Variation of the Thickness
- Stability of Proteins Confined in MCM-48 Mesoporous Molecular Sieves – The Effects of pH, Temperature and Co-solvents
- Hydrogen Bonding of Water Confined in Controlled-Pore Glass 10-75 Studied by 1H-Solid State NMR
