Unrevealing the potential of fibrous biomaterials in cartilage tissue engineering: a review
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Sachin S. Mali
, Dipak S. Thorat
, Anil Kumar Singh
, Akshay Kundale
Abstract
Fibrous biomaterials have showed considerable potential in cartilage tissue engineering due to their ability to imitate the structure and characteristics of the original extracellular matrix. Sustainable biomaterials such as chitosan, silk fibroin, and collagen can be produced into a variety of shapes, including hydrogels, scaffolds, and electrospun nanofibers, to develop an optimal milieu for chondrocyte adhesion, proliferation, and cartilage matrix deposition. In recent years, various studies showed that biomaterials-based fiber mats obtained through electrospinning as scaffolds exhibit remarkable chondrocyte growth support. These fiber mats promote high chondrocyte viability and cell proliferation, particularly when thin neutralized fibers are utilized. The biomimetic attributes of these biomaterials obtained from renewable resources such as plants, animals, and microbes have intrinsic benefits such as biocompatibility, microstructure resemblance to the original extracellular matrix, and adjustable mechanical properties. However, there are still hurdles in optimizing scaffold–cell interactions, controlled degradation, stress response, and flexibility for successful clinical translation. As a result, fibrous biomaterials exhibit significant potential for cartilage tissue engineering by promoting chondrocyte adhesion, proliferation, and cartilage matrix deposition. Nonetheless, additional study is required to solve the obstacles and optimize these materials for successful clinical applications.
Acknowledgments
This work was partially supported by CMU proactive Researcher Scheme (2023), Chiang Mai University, Chiang Mai for Sudarshan Singh.
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Research ethics: Not applicable.
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Informed consent: Not applicable.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Use of Large Language Models, AI and Machine Learning Tools: None declared.
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Conflict of interest: The authors state no conflict of interest.
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Research funding: None declared.
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Data availability: The raw data can be obtained on request from the corresponding author.
References
1. Brovold, M, Almeida, JI, Pla-Palacín, I, Sainz-Arnal, P, Sánchez-Romero, N, Rivas, JJ, et al.. Naturally-derived biomaterials for tissue engineering applications. Adv Exp Med Biol 2018;1077:421. https://doi.org/10.1007/978-981-13-0947-2_23.Search in Google Scholar PubMed PubMed Central
2. Garcia Garcia, CE, Lardy, B, Bossard, F, Soltero Martínez, FA, Rinaudo, M. Chitosan based biomaterials for cartilage tissue engineering: chondrocyte adhesion and proliferation. Food Hydrocolloids for Health 2021;1:100018. https://doi.org/10.1016/J.FHFH.2021.100018.Search in Google Scholar
3. Camarero-Espinosa, S, Rothen-Rutishauser, B, Foster, EJ, Weder, C. Articular cartilage: from formation to tissue engineering. Biomater Sci 2016;4:734–67. https://doi.org/10.1039/C6BM00068A.Search in Google Scholar
4. Betz, RR. Limitations of autograft and allograft: new synthetic solutions. Orthopedics 2002;25. https://doi.org/10.3928/0147-7447-20020502-04.Search in Google Scholar PubMed
5. Campos, Y, Almirall, A, Fuentes, G, Bloem, HL, Kaijzel, EL, Cruz, LJ. Tissue engineering: an alternative to repair cartilage. Tissue Eng Part B 2019;25:357–73. https://doi.org/10.1089/TEN.TEB.2018.0330.Search in Google Scholar
6. Djuraeva, B, Omonov, Z, Saidullaev, E. The function of joints in the body. Евразийский Журнал Медицинских и Естественных Наук 2023;3:196–204. https://doi.org/10.5281/ZENODO.5884973.Search in Google Scholar
7. García-Carvajal, ZY, Garciadiego-Cázares, D, Parra- Cid, C, Aguilar-Gaytán, R, Velasquillo, C, C, I, et al.. Cartilage tissue engineering: the role of extracellular matrix (ECM) and novel strategies, J Regen Med Tissue Eng 2013. https://doi.org/10.5772/55917.Search in Google Scholar
8. Xiong, Y, Bin Mi, B, Lin, Z, Hu, YQ, Yu, L, Zha, KK, et al.. The role of the immune microenvironment in bone, cartilage, and soft tissue regeneration: from mechanism to therapeutic opportunity. Mil Med Res 2022;9. https://doi.org/10.1186/S40779-022-00426-8.Search in Google Scholar PubMed PubMed Central
9. Roberts, J. Cells at work. In: Mastering human biology. Macmillan master series. London: Palgrave; 1991: 24–48 pp.10.1007/978-1-349-11386-6_2Search in Google Scholar
10. Krakowski, P, Rejniak, A, Sobczyk, J, Karpiński, R. Cartilage integrity: a review of mechanical and frictional properties and repair approaches in osteoarthritis. Healthcare (Basel) 2024;12. https://doi.org/10.3390/HEALTHCARE12161648.Search in Google Scholar PubMed PubMed Central
11. Windt, TS, Saris, DBF. Treatment algorithm for articular cartilage repair of the knee: towards patient profiling using evidence-based tools. In: Techniques in cartilage repair surgery. Berlin, Heidelberg: Springer; 2014.Search in Google Scholar
12. Buchanan, JL. Types of fibrocartilage. Clin Podiatr Med Surg 2022;39:357–61. https://doi.org/10.1016/J.CPM.2022.02.001.Search in Google Scholar
13. Dehghani, F, Fathi, A. Challenges for cartilage regeneration. Springer Series in Biomaterials Science and Engineering 2016;8:389–466. https://doi.org/10.1007/978-3-662-53574-5_14.Search in Google Scholar
14. Ds, L, DA, S, KL, B, Rs, H, H, SG, J, E, et al.. Knee pain and mobility impairments: meniscal and articular cartilage lesions revision 2018. J Orthop Sports Phys Ther 2018;48:125. https://doi.org/10.2519/JOSPT.2018.0301.Search in Google Scholar
15. Yuh, C, Wimmer, MA. Chapter 4 – Cartilage tribology and friction coefficient. In: Nochehdehi, AR, Nemavhola, F, Thomas, S, Maria, HJ, editors. Cartilage tissue and knee joint biomechanics. Cambridge: Academic Press; 2024:37–45 pp.10.1016/B978-0-323-90597-8.00008-6Search in Google Scholar
16. Xu, M, Qin, M, Cheng, Y, Niu, X, Kong, J, Zhang, X, et al.. Alginate microgels as delivery vehicles for cell-based therapies in tissue engineering and regenerative medicine. Carbohydr Polym 2021;266:118128. https://doi.org/10.1016/J.CARBPOL.2021.118128.Search in Google Scholar
17. Rahmani Del Bakhshayesh, A, Babaie, S, Tayefi Nasrabadi, H, Asadi, N, Akbarzadeh, A, Abedelahi, A. An overview of various treatment strategies, especially tissue engineering for damaged articular cartilage. Artif Cells, Nanomed Biotechnol 2020;48:1089–104. https://doi.org/10.1080/21691401.2020.1809439.Search in Google Scholar PubMed
18. Josephson, CD, Kuehnert, MJ. Human tissue allografts: responsibilities in understanding the path from donor to recipient. Rossi’s Principles of Transfusion Medicine 2022:660–73. https://doi.org/10.1002/9781119719809.CH58.Search in Google Scholar
19. Zhou, J, Li, Q, Tian, Z, Yao, Q, Zhang, M. Recent advances in 3D bioprinted cartilage-mimicking constructs for applications in tissue engineering. Mater Today Bio 2023;23:100870. https://doi.org/10.1016/J.MTBIO.2023.100870.Search in Google Scholar
20. Abraham, N, Pandey, G, Kolipaka, T, Negi, M, Srinivasarao, DA, Srivastava, S. Exploring advancements in polysaccharide-based approaches: the cornerstone of next-generation cartilage regeneration therapeutics. Int J Biol Macromol 2025;306:141352. https://doi.org/10.1016/J.IJBIOMAC.2025.141352.Search in Google Scholar
21. Armiento, AR, Stoddart, MJ, Alini, M, Eglin, D. Biomaterials for articular cartilage tissue engineering: learning from biology. Acta Biomater 2018;65:1–20. https://doi.org/10.1016/J.ACTBIO.2017.11.021.Search in Google Scholar
22. Sharma, R, Malviya, R, Singh, S, Prajapati, B. A critical review on classified excipient sodium-alginate-based hydrogels: modification, characterization, and application in soft tissue engineering. Gels 2023;9:430. https://doi.org/10.3390/GELS9050430.Search in Google Scholar
23. Mohite, P, Puri, A, Munde, S, Dave, R, Khan, S, Patil, R, et al.. Potential of Chitosan/gelatin-based nanofibers in delivering drugs for the management of varied complications: a review, Polymers 2025;17, Page 435 17:435. https://doi.org/10.3390/POLYM17040435.Search in Google Scholar PubMed PubMed Central
24. Basu, B, Rahaman, M, Ghosh, S, Dutta, S, Kumar, A, Mukherjee, S, et al.. Emerging silk sericin-based formulation fortified with therapeutics in the management of diabetic wound and skin tissue regeneration. Zeitschrift Fur Naturforschung - Section C J Biosci 2025. https://doi.org/10.1515/ZNC-2024-0198/MACHINEREADABLECITATION/RIS.Search in Google Scholar
25. Del Bakhshayesh, AR, Asadi, N, Alihemmati, A, Tayefi Nasrabadi, H, Montaseri, A, Davaran, S, et al.. An overview of advanced biocompatible and biomimetic materials for creation of replacement structures in the musculoskeletal systems: focusing on cartilage tissue engineering. J Biol Eng 2019 13:1–21. https://doi.org/10.1186/S13036-019-0209-9.Search in Google Scholar PubMed PubMed Central
26. Vyas, J, Raytthatha, N, Vyas, P, Prajapati, BG, Uttayarat, P, Singh, S, et al.. Biomaterial-based additive manufactured composite/scaffolds for tissue engineering and regenerative medicine: a comprehensive review. Polymers 2025;17:1090. 2025, Page 1090 17 https://doi.org/10.3390/POLYM17081090.Search in Google Scholar PubMed PubMed Central
27. Ullah, S, Chen, X. Fabrication, applications and challenges of natural biomaterials in tissue engineering. Appl Mater Today 2020;20:100656. https://doi.org/10.1016/J.APMT.2020.100656.Search in Google Scholar
28. Venugopal, D, Vishwakarma, S, Kaur, I, Samavedi, S. Electrospun fiber-based strategies for controlling early innate immune cell responses: towards immunomodulatory mesh designs that facilitate robust tissue repair. Acta Biomater 2023;163:228–47. https://doi.org/10.1016/J.ACTBIO.2022.06.004.Search in Google Scholar PubMed
29. Gentili/snm, C, Cancedda, R. Cartilage and bone extracellular matrix. Curr Pharm Des 2009;15:1334–48. https://doi.org/10.2174/138161209787846739.Search in Google Scholar PubMed
30. Muzzarelli, RAA, Greco, F, Busilacchi, A, Sollazzo, V, Gigante, A. Chitosan, hyaluronan and chondroitin sulfate in tissue engineering for cartilage regeneration: a review. Carbohydr Polym 2012;89:723–39. https://doi.org/10.1016/J.CARBPOL.2012.04.057.Search in Google Scholar
31. Altman, GH, Diaz, F, Jakuba, C, Calabro, T, Horan, RL, Chen, J, et al.. Silk-based biomaterials. Biomaterials 2003;24:401–16. https://doi.org/10.1016/S0142-9612(02)00353-8.Search in Google Scholar
32. Bicho, D, Ajami, S, Liu, C, Reis, RL, Oliveira, JM. Peptide-biofunctionalization of biomaterials for osteochondral tissue regeneration in early stage osteoarthritis: challenges and opportunities. J Mater Chem B 2019;7:1027–44. https://doi.org/10.1039/C8TB03173H.Search in Google Scholar
33. Pizzo, AM, Kokini, K, Vaughn, LC, Waisner, BZ, Voytik-Harbin, SL. Extracellular matrix (ECM) microstructural composition regulates local cell-ECM biomechanics and fundamental fibroblast behavior: a multidimensional perspective. J Appl Physiol 1985;98:1909–21. https://doi.org/10.1152/JAPPLPHYSIOL.01137.2004.Search in Google Scholar
34. Guarino, V, Cirillo, V, Ambrosio, L. Bicomponent electrospun scaffolds to design extracellular matrix tissue analogs. Expet Rev Med Dev 2016;13:83–102. https://doi.org/10.1586/17434440.2016.1126505.Search in Google Scholar PubMed
35. Radhakrishnan, S, Nagarajan, S, Bechelany, M, Kalkura, SN. Collagen based biomaterials for tissue engineering applications: a review. Lecture Notes in Earth System Sciences 2020:3–22. https://doi.org/10.1007/978-3-030-21614-6_1.Search in Google Scholar
36. McCullen, SD, Autefage, H, Callanan, A, Gentleman, E, Stevens, MM. Anisotropic fibrous scaffolds for articular cartilage regeneration. Tissue Eng 2012;18:2073–83. https://doi.org/10.1089/TEN.TEA.2011.0606.Search in Google Scholar
37. Li, G, Li, Y, Chen, G, He, J, Han, Y, Wang, X, et al.. Silk-based biomaterials in biomedical textiles and fiber-based implants. Adv Healthcare Mater 2015;4:1134. https://doi.org/10.1002/ADHM.201500002.Search in Google Scholar
38. Silk biomaterials. Department of Civil and Environmental Engineering, (n.d.). https://engineering.tufts.edu/cee/msml/research/silk-biomaterials (Accessed February 3, 2025).Search in Google Scholar
39. Mohite, P, Shah, SR, Singh, S, Rajput, T, Munde, S, Ade, N, et al.. Chitosan and chito-oligosaccharide: a versatile biopolymer with endless grafting possibilities for multifarious applications. Front Bioeng Biotechnol 2023;11:1190879. https://doi.org/10.3389/FBIOE.2023.1190879/XML.Search in Google Scholar
40. Chakravarty, J, Edwards, TA. Innovation from waste with biomass-derived chitin and chitosan as green and sustainable polymer: a review. Energy Nexus 2022;8. https://doi.org/10.1016/J.NEXUS.2022.100149.Search in Google Scholar
41. Debari, MK, King, CI, Altgold, TA, Abbott, RD. Silk fibroin as a green material. ACS Biomater Sci Eng 2021;7:3530–44. https://doi.org/10.1021/ACSBIOMATERIALS.1C00493.Search in Google Scholar PubMed
42. Noorzai, S, Verbeek, CJR, Noorzai, S, Verbeek, CJR. Collagen: from waste to gold. Biotechnological Applications of Biomass 2020. https://doi.org/10.5772/INTECHOPEN.94266.Search in Google Scholar
43. Gomes, V, Salgueiro, SP. From small to large-scale: a review of recombinant spider silk and collagen bioproduction. Discov Mater 2022;2:1–24. https://doi.org/10.1007/S43939-022-00024-4/TABLES/4.Search in Google Scholar
44. Zhao, W, Jin, X, Cong, Y, Liu, Y, Fu, J. Degradable natural polymer hydrogels for articular cartilage tissue engineering. J Chem Tech Biotechnol 2013;88:327–39. https://doi.org/10.1002/JCTB.3970.Search in Google Scholar
45. Iber, BT, Kasan, NA, Torsabo, D, Omuwa, JW. A review of various sources of chitin and chitosan in nature. J Renew Mater 2022;10:1097–123. https://doi.org/10.32604/JRM.2022.018142.Search in Google Scholar
46. Hafezi, M, Khorasani, SN, Zare, M, Neisiany, RE, Davoodi, P. Advanced hydrogels for cartilage tissue engineering: recent progress and future directions. Polymers 2021;13, Page 4199 13:4199. https://doi.org/10.3390/POLYM13234199.Search in Google Scholar
47. Dutta, PK, Rinki, K, Dutta, J. Chitosan: a promising biomaterial for tissue engineering scaffolds. Adv Polym Sci 2011;244:45–80. https://doi.org/10.1007/12_2011_112.Search in Google Scholar
48. Mangat, AS, Singh, S, Gupta, M, Sharma, R. Experimental investigations on natural fiber embedded additive manufacturing-based biodegradable structures for biomedical applications. Rapid Prototyp J 2018;24:1221–34. https://doi.org/10.1108/RPJ-08-2017-0162.Search in Google Scholar
49. Attri, K, Shruthi, GH, Gulabrao, DP, Teja, KS, Garai, I, Pandey, AK, et al.. Silk biomaterials: applications and future prospects in biomedical engineering. Biomed Eng;45:205–16. - Google Search, (n.d.) (Accessed 4 February 2025)10.56557/upjoz/2024/v45i164301Search in Google Scholar
50. Carr, BP, Chen, Z, Chung, JHY, Wallace, GG. Collagen alignment via electro-compaction for biofabrication applications: a review. Polymers 2022:14, Page 4270 1:4270. https://doi.org/10.3390/POLYM14204270.Search in Google Scholar PubMed PubMed Central
51. Sun, L, Xu, Y, Han, Y, Cui, J, Jing, Z, Li, D, et al.. Collagen‐based hydrogels for cartilage regeneration. Orthop Surg 2023;15:3026. https://doi.org/10.1111/OS.13884.Search in Google Scholar PubMed PubMed Central
52. He, JM, Zhu, PF, Li, LH, Wang, Z, Li, XL, Wang, S, et al.. Silk fibroin/chitosan/TGF-β1-loaded microsphere scaffolds for cartilage reparation. Bio Med Mater Eng 2021;32:347–58. https://doi.org/10.3233/BME-201178.Search in Google Scholar PubMed
53. Paladini, F, Pollini, M. Novel approaches and biomaterials for bone tissue engineering: a focus on silk fibroin. Materials 2022;15, Page 6952 15:6952. https://doi.org/10.3390/MA15196952.Search in Google Scholar PubMed PubMed Central
54. Das, U, Kapoor, DU, Singh, S, Prajapati, BG. Unveiling the potential of chitosan-coated lipid nanoparticles in drug delivery for management of critical illness: a review. Zeitschrift Fur Naturforschung - Section C Journal of Biosciences 2024;79:107–24. https://doi.org/10.1515/ZNC-2023-0181/MACHINEREADABLECITATION/RIS.Search in Google Scholar
55. Islam, MM, Shahruzzaman, M, Biswas, S, Nurus Sakib, M, Rashid, TU. Chitosan based bioactive materials in tissue engineering applications-A review. Bioact Mater 2020;5:164–83. https://doi.org/10.1016/J.BIOACTMAT.2020.01.012.Search in Google Scholar
56. Singh, S, Nwabor, OF, Syukri, DM, Voravuthikunchai, SP. Chitosan-poly(vinyl alcohol) intelligent films fortified with anthocyanins isolated from Clitoria ternatea and Carissa carandas for monitoring beverage freshness. Int J Biol Macromol 2021;182:1015–25. https://doi.org/10.1016/J.IJBIOMAC.2021.04.027.Search in Google Scholar PubMed
57. Eze, FN, Jayeoye, TJ, Singh, S. Fabrication of intelligent pH-sensing films with antioxidant potential for monitoring shrimp freshness via the fortification of chitosan matrix with broken riceberry phenolic extract. Food Chem 2022;366:130574. https://doi.org/10.1016/J.FOODCHEM.2021.130574.Search in Google Scholar
58. Nwabor, OF, Singh, S, Paosen, S, Vongkamjan, K, Voravuthikunchai, SP. Enhancement of food shelf life with polyvinyl alcohol-chitosan nanocomposite films from bioactive eucalyptus leaf extracts. Food Biosci 2020;36:100609. https://doi.org/10.1016/J.FBIO.2020.100609.Search in Google Scholar
59. Serda, M, Becker, FG, Cleary, M, Team, RM, Holtermann, H, The, D, et al.. Synteza i aktywność biologiczna nowych analogów tiosemikarbazonowych chelatorów żelaza. Uniwersytet Śląski 2013;7:343–54. https://doi.org/10.2/JQUERY.MIN.JS.Search in Google Scholar
60. Theocharis, AD, Skandalis, SS, Gialeli, C, Karamanos, NK. Extracellular matrix structure. Adv Drug Deliv Rev 2016;97:4–27. https://doi.org/10.1016/J.ADDR.2015.11.001.Search in Google Scholar PubMed
61. Cheng, G, Dai, J, Dai, J, Wang, H, Chen, S, liu, Y, et al.. Extracellular matrix imitation utilizing nanofibers-embedded biomimetic scaffolds for facilitating cartilage regeneration. ChEnJ 2021;410:128379. https://doi.org/10.1016/J.CEJ.2020.128379.Search in Google Scholar
62. Abourehab, MAS, Pramanik, S, Abdelgawad, MA, Abualsoud, BM, Kadi, A, Ansari, MJ, et al.. Recent advances of chitosan formulations in biomedical applications. Int J Mol Sci 2022;23. https://doi.org/10.3390/IJMS231810975.Search in Google Scholar PubMed PubMed Central
63. Shen, Y, Xu, Y, Yi, B, Wang, X, Tang, H, Chen, C, et al.. Engineering a highly biomimetic chitosan-based cartilage scaffold by using short fibers and a cartilage-decellularized matrix. Biomacromolecules 2021;22:2284–97. https://doi.org/10.1021/ACS.BIOMAC.1C00366.Search in Google Scholar PubMed
64. Phan, VHG, Murugesan, M, Nguyen, PPT, Luu, CH, Le, NHH, Nguyen, HT, et al.. Biomimetic injectable hydrogel based on silk fibroin/hyaluronic acid embedded with methylprednisolone for cartilage regeneration. Colloids Surf B Biointerfaces 2022;219. https://doi.org/10.1016/J.COLSURFB.2022.112859.Search in Google Scholar
65. Rajagopal, K, Ramesh, S, Walter, NM, Arora, A, Katti, DS, Madhuri, V. In vivo cartilage regeneration in a multi-layered articular cartilage architecture mimicking scaffold. Bone Joint Res 2020;9:601–12. https://doi.org/10.1302/2046-3758.99.BJR-2019-0210.R2/LETTERTOEDITOR.Search in Google Scholar
66. Guo, C, Cao, Z, Peng, Y, Wu, R, Xu, H, Yuan, Z, et al.. Subchondral bone-inspired hydrogel scaffold for cartilage regeneration. Colloids Surf B Biointerfaces 2022;218:112721. https://doi.org/10.1016/J.COLSURFB.2022.112721.Search in Google Scholar PubMed
67. Singh, S, Supaweera, N, Nwabor, OF, Chaichompoo, W, Suksamrarn, A, Chittasupho, C, et al.. Poly (vinyl alcohol)-gelatin-sericin copolymerized film fortified with vesicle-entrapped demethoxycurcumin/bisdemethoxycurcumin for improved stability, antibacterial, anti-inflammatory, and skin tissue regeneration. Int J Biol Macromol 2024;258:129071. https://doi.org/10.1016/J.IJBIOMAC.2023.129071.Search in Google Scholar PubMed
68. Sun, W, Gregory, DA, Tomeh, MA, Zhao, X. Silk fibroin as a functional biomaterial for tissue engineering. Int J Mol Sci 2021;22:1499. https://doi.org/10.3390/IJMS22031499.Search in Google Scholar PubMed PubMed Central
69. Zheng, D, Chen, T, Han, L, Lv, S, Yin, J, Yang, K, et al.. Synergetic integrations of bone marrow stem cells and transforming growth factor-β1 loaded chitosan nanoparticles blended silk fibroin injectable hydrogel to enhance repair and regeneration potential in articular cartilage tissue. Int Wound J 2022;19:1023–38. https://doi.org/10.1111/IWJ.13699.Search in Google Scholar PubMed PubMed Central
70. Lyu, Y, Liu, Y, He, H, Wang, H. Application of silk-fibroin-based hydrogels in tissue engineering. Gels 2023;9:431. https://doi.org/10.3390/GELS9050431.Search in Google Scholar
71. Zhou, Z, Cui, J, Wu, S, Geng, Z, Su, J. Silk fibroin-based biomaterials for cartilage/osteochondral repair. Theranostics 2022;12:5103. https://doi.org/10.7150/THNO.74548.Search in Google Scholar PubMed PubMed Central
72. Janani, G, Kumar, M, Chouhan, D, Moses, JC, Gangrade, A, Bhattacharjee, S, et al.. Insight into silk-based biomaterials: from physicochemical attributes to recent biomedical applications. ACS Appl Bio Mater 2019;2:5460–91. https://doi.org/10.1021/ACSABM.9B00576.Search in Google Scholar
73. Ramírez Rodríguez, GB, Patrício, TMF, López, JMD. 8 – Natural polymers for bone repair. In: Pawelec, KM, Planell, JA, editors. Woodhead Publishing series in biomaterials, bone repair biomaterials, 2nd ed. Sawston: Woodhead publishing; 2019:199–232 pp.10.1016/B978-0-08-102451-5.00008-1Search in Google Scholar
74. Farokhi, M, Mottaghitalab, F, Fatahi, Y, Saeb, MR, Zarrintaj, P, Kundu, SC, et al.. Silk fibroin scaffolds for common cartilage injuries: possibilities for future clinical applications. Eur Polym J 2019;115:251–67. https://doi.org/10.1016/J.EURPOLYMJ.2019.03.035.Search in Google Scholar
75. Agrawal, P, Pramanik, K, Biswas, A, Ku Patra, R. In vitro cartilage construct generation from silk fibroin- chitosan porous scaffold and umbilical cord blood derived human mesenchymal stem cells in dynamic culture condition. J Biomed Mater Res 2018;106:397–407. https://doi.org/10.1002/JBM.A.36253.Search in Google Scholar
76. Yang, YJ, Kwon, Y, Choi, BH, Jung, D, Seo, JH, Lee, KH, et al.. Multifunctional adhesive silk fibroin with blending of RGD-bioconjugated mussel adhesive protein. Biomacromolecules 2014;15:1390–8. https://doi.org/10.1021/BM500001N/ASSET/IMAGES/MEDIUM/BM-2014-00001N_0009.GIF.Search in Google Scholar
77. Karamanos, NK, Theocharis, AD, Piperigkou, Z, Manou, D, Passi, A, Skandalis, SS, et al.. A guide to the composition and functions of the extracellular matrix. FEBS J 2021;288:6850–912. https://doi.org/10.1111/FEBS.15776.Search in Google Scholar
78. Alcaide-Ruggiero, L, Molina-Hernández, V, Granados, MM, Domínguez, JM. Main and minor types of collagens in the articular cartilage: the role of collagens in repair tissue evaluation in chondral defects. Int J Mol Sci 2021;22:13329. https://doi.org/10.3390/IJMS222413329.Search in Google Scholar PubMed PubMed Central
79. Cen, L, Liu, W, Cui, L, Zhang, W, Cao, Y. Collagen tissue engineering: development of novel biomaterials and applications. Pediatr Res 2008;63:492–6. https://doi.org/10.1203/PDR.0B013E31816C5BC3.Search in Google Scholar
80. Bacakova, L, Filova, E, Parizek, M, Ruml, T, Svorcik, V. Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants. Biotechnol Adv 2011;29:739–67. https://doi.org/10.1016/J.BIOTECHADV.2011.06.004.Search in Google Scholar
81. Browe, DC, Díaz-Payno, PJ, Freeman, FE, Schipani, R, Burdis, R, Ahern, DP, et al.. Bilayered extracellular matrix derived scaffolds with anisotropic pore architecture guide tissue organization during osteochondral defect repair. Acta Biomater 2022;143:266–81. https://doi.org/10.1016/J.ACTBIO.2022.03.009.Search in Google Scholar
82. Cai, R, Shan, Y, Du, F, Miao, Z, Zhu, L, Hang, L, et al.. Injectable hydrogels as promising in situ therapeutic platform for cartilage tissue engineering. Int J Biol Macromol 2024;261. https://doi.org/10.1016/J.IJBIOMAC.2024.129537.Search in Google Scholar
83. Irawan, V, Sung, TC, Higuchi, A, Ikoma, T. Collagen scaffolds in cartilage tissue engineering and relevant approaches for future development. Tissue Eng Regen Med 2018;15:673–97. https://doi.org/10.1007/S13770-018-0135-9.Search in Google Scholar PubMed PubMed Central
84. Chen, Y, Ma, M, Cao, H, Wang, Y, Xu, Y, Teng, Y, et al.. Identification of endogenous migratory MSC-like cells and their interaction with the implant materials guiding osteochondral defect repair. J Mater Chem B 2019;7:3993–4007. https://doi.org/10.1039/C9TB00674E.Search in Google Scholar
85. Xiong, L, Wang, H, Wang, J, Luo, J, Xie, R, Lu, F, et al.. Facilely prepared thirsty granules arouse tough wet adhesion on overmoist wounds for hemostasis and tissue repair. ACS Appl Mater Interfaces 2023;15:49035–50. https://doi.org/10.1021/ACSAMI.3C11403/SUPPL_FILE/AM3C11403_SI_007.MP4.Search in Google Scholar
86. Xu, F, Dawson, C, Lamb, M, Mueller, E, Stefanek, E, Akbari, M, et al.. Hydrogels for tissue engineering: addressing key design needs toward clinical translation. Front Bioeng Biotechnol 2022;10:849831. https://doi.org/10.3389/FBIOE.2022.849831/XML.Search in Google Scholar
87. Farokhi, M, Jonidi Shariatzadeh, F, Solouk, A, Mirzadeh, H. Alginate based scaffolds for cartilage tissue engineering: a review. International Journal of Polymeric Materials and Polymeric Biomaterials 2020;69:230–47. https://doi.org/10.1080/00914037.2018.1562924.Search in Google Scholar
88. Esmaeili, Y, Bidram, E, Bigham, A, Atari, M, Nasr Azadani, R, Tavakoli, M, et al.. Exploring the evolution of tissue engineering strategies over the past decade: from cell-based strategies to gene-activated matrix. Alex Eng J 2023;81:137–69. https://doi.org/10.1016/J.AEJ.2023.08.080.Search in Google Scholar
89. Yang, C, Chen, R, Chen, C, Yang, F, Xiao, H, Geng, B, et al.. Tissue engineering strategies hold promise for the repair of articular cartilage injury. Biomed Eng Online 2024 23:1–39. https://doi.org/10.1186/S12938-024-01260-W.Search in Google Scholar PubMed PubMed Central
90. Mukherjee, S, Karati, D, Ganguly, SC, Chakrabarty, S, Bhattacharya, J, Singh, S, et al.. Unlocking the potential of engineered biopolymer-based nanofibers for the management of diabetic wounds: a review. Regen Eng Transl Med 2025:1–20. https://doi.org/10.1007/S40883-025-00453-6/METRICS.Search in Google Scholar
91. Ma, Y, Zhou, R, Yang, M, Zhang, J, Song, W, Ma, X, et al.. Electrospinning-based bone tissue scaffold construction: progress and trends. Mater Des 2025;252:113792. https://doi.org/10.1016/J.MATDES.2025.113792.Search in Google Scholar
92. Teng, Y, Song, L, Shi, J, Lv, Q, Hou, S, Ramakrishna, S. Advancing electrospinning towards the future of biomaterials in biomedical engineering. Regen Biomater 2025;12:rbaf034. https://doi.org/10.1093/RB/RBAF034.Search in Google Scholar PubMed PubMed Central
93. Mohite, P, Puri, A, Munde, S, Dave, R, Khan, S, Patil, R, et al.. Potential of Chitosan/gelatin-based nanofibers in delivering drugs for the management of varied complications: a review, Polymers 2025;17, Page 435 17:435. https://doi.org/10.3390/POLYM17040435.Search in Google Scholar
94. Datta, D, Bandi, SP, Colaco, V, Dhas, N, Saha, SS, Hussain, SZ, et al.. Cellulose-based nanofibers infused with biotherapeutics for enhanced wound-healing applications. ACS Polymers Au 2025;5:80–104. https://doi.org/10.1021/ACSPOLYMERSAU.4C00092/ASSET/IMAGES/LARGE/LG4C00092_0017.JPEG.Search in Google Scholar
95. Anusiya, G, Jaiganesh, R. A review on fabrication methods of nanofibers and a special focus on application of cellulose nanofibers. Carbohydrate Polymer Technologies and Applications 2022;4:100262. https://doi.org/10.1016/J.CARPTA.2022.100262.Search in Google Scholar
96. Rana, D, Ratheesh, G, Ramakrishna, S, Ramalingam, M. Nanofiber composites in cartilage tissue engineering, Nanofiber Composites for Biomedical Applications; 2017:325–344. https://doi.org/10.1016/B978-0-08-100173-8.00013-2.Search in Google Scholar
97. Ahmadian, E, Eftekhari, A, Janas, D, Vahedi, P. Nanofiber scaffolds based on extracellular matrix for articular cartilage engineering: a perspective. Nanotheranostics 2023;7:61–9. https://doi.org/10.7150/NTNO.78611.Search in Google Scholar PubMed PubMed Central
98. Wang, L, Qiu, Y, Guo, Y, Si, Y, Liu, L, Cao, J, et al.. Smart, elastic, and Nanofiber-based 3D scaffolds with self-deploying capability for osteoporotic bone regeneration. Nano Lett 2019;19:9112–20. https://doi.org/10.1021/ACS.NANOLETT.9B04313/SUPPL_FILE/NL9B04313_SI_001.PDF.Search in Google Scholar
99. Liang, R, Zhao, J, Li, B, Cai, P, Loh, XJ, Xu, C, et al.. Implantable and degradable antioxidant poly(ε-caprolactone)-lignin nanofiber membrane for effective osteoarthritis treatment. Biomaterials 2020;230:119601. https://doi.org/10.1016/J.BIOMATERIALS.2019.119601.Search in Google Scholar
100. Vyas, J, Singh, S, Shah, I, Prajapati, BG, Potential applications and additive manufacturing technology-based considerations of mesoporous silica: a review, AAPS PharmSciTech 2023 25 1–27. https://doi.org/10.1208/S12249-023-02720-7.Search in Google Scholar
101. Shah, SR, Modi, CD, Singh, S, Mori, DD, Soniwala, MM, Prajapati, BG. Recent advances in additive manufacturing of polycaprolactone-based scaffolds for tissue engineering applications: a comprehensive review, Regen Eng Transl Med 2024 11:112–31. https://doi.org/10.1007/S40883-024-00351-3.Search in Google Scholar
102. Dan, X, Chen, H, Li, S, Xue, P, Liu, B, Zhang, Z, et al.. Silk fibroin as a 3D printing bioink for tissue engineering applications. Appl Mater Today 2025;44:102775. https://doi.org/10.1016/J.APMT.2025.102775.Search in Google Scholar
103. Vernerey, FJ, Lalitha Sridhar, S, Muralidharan, A, Bryant, SJ. Mechanics of 3D cell-hydrogel interactions: experiments, models, and mechanisms. Chem Rev 2021;121:11085–148. https://doi.org/10.1021/ACS.CHEMREV.1C00046.Search in Google Scholar PubMed
104. Advanced scaffold design techniques, (n.d.). https://www.numberanalytics.com/blog/advanced-scaffold-design-techniques (Accessed July 20, 2025).Search in Google Scholar
105. Hashemi-Afzal, F, Fallahi, H, Bagheri, F, Collins, MN, Eslaminejad, MB, Seitz, H. Advancements in hydrogel design for articular cartilage regeneration: a comprehensive review. Bioact Mater 2025;43:1–31. https://doi.org/10.1016/J.BIOACTMAT.2024.09.005.Search in Google Scholar
106. Thorat, DS, Ushir, YV, Singh, S. Value-added-peanut shell as potential source for biofilters: an eco-friendly way to clean water and manage nutrients, Biotech Sust Mater 2025;2:1–18. https://doi.org/10.1186/S44316-025-00034-1.Search in Google Scholar
107. Thorat, DS, Singh, S, Ushir, YV, Tiwari, K, kokate, S, Nagime, PV. Biomaterials-based biofilters from sugarcane waste: an eco-friendly way to clean water and manage nutrients, Discov Mater 2025;5:1–26. https://doi.org/10.1007/S43939-025-00234-6.Search in Google Scholar
108. Liu, M, Zeng, X, Ma, C, Yi, H, Ali, Z, Mou, X, et al.. Injectable hydrogels for cartilage and bone tissue engineering, Bone Res 2017;5:1–20. https://doi.org/10.1038/boneres.2017.14.Search in Google Scholar PubMed PubMed Central
109. Sen Shen, Z, Cui, X, Hou, RX, Li, Q, Deng, HX, Fu, J. Tough biodegradable chitosan–gelatin hydrogels via in situ precipitation for potential cartilage tissue engineering. RSC Adv 2015;5:55640–7. https://doi.org/10.1039/C5RA06835E.Search in Google Scholar
110. Naderi-Meshkin, H, Andreas, K, Matin, MM, Sittinger, M, Bidkhori, HR, Ahmadiankia, N, et al.. Chitosan-based injectable hydrogel as a promising in situ forming scaffold for cartilage tissue engineering. Cell Biol Int 2014;38:72–84. https://doi.org/10.1002/CBIN.10181.Search in Google Scholar PubMed
111. Sá-Lima, H, Caridade, SG, Mano, JF, Reis, RL. Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration. Soft Matter 2010;6:5184–95. https://doi.org/10.1039/C0SM00041H.Search in Google Scholar
112. Moreira, CDF, Carvalho, SM, Mansur, HS, Pereira, MM. Thermogelling chitosan–collagen–bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering. Mater Sci Eng C 2016;58:1207–16. https://doi.org/10.1016/J.MSEC.2015.09.075.Search in Google Scholar
113. Yang, X, Liu, Q, Chen, X, Yu, F, Zhu, Z. Investigation of PVA/ws-chitosan hydrogels prepared by combined γ-irradiation and freeze-thawing. Carbohydr Polym 2008;73:401–8. https://doi.org/10.1016/J.CARBPOL.2007.12.008.Search in Google Scholar
114. Funayama, A, Niki, Y, Matsumoto, H, Maeno, S, Yatabe, T, Morioka, H, et al.. Repair of full-thickness articular cartilage defects using injectable type II collagen gel embedded with cultured chondrocytes in a rabbit model. J Orthop Sci 2008;13:225–32. https://doi.org/10.1007/S00776-008-1220-Z.Search in Google Scholar
115. Kontturi, LS, Järvinen, E, Muhonen, V, Collin, EC, Pandit, AS, Kiviranta, I, et al.. An injectable, in situ forming type II collagen/hyaluronic acid hydrogel vehicle for Chondrocyte delivery in cartilage tissue engineering. Drug Deliv Transl Res 2014;4:149–58. https://doi.org/10.1007/S13346-013-0188-1/METRICS.Search in Google Scholar
116. Xiang, C, Guo, Z, Zhang, Q, Wang, Z, Li, X, Chen, W, et al.. Physically crosslinked poly(vinyl alcohol)-based hydrogels for cartilage tissue engineering. Mater Des 2024;243:113048. https://doi.org/10.1016/J.MATDES.2024.113048.Search in Google Scholar
117. Liu, H, Dou, Y, Wei, J, Xiao, S, Jin, S, Yuan, L, et al.. Fiber-reinforced hydrogel combined with 3D printed scaffolds for regeneration of osteochondral defects. Mater Chem Phys 2025;335:130532. https://doi.org/10.1016/J.MATCHEMPHYS.2025.130532.Search in Google Scholar
118. Dubey, AK, Mostafavi, E. Biomaterials-mediated CRISPR/Cas9 delivery: recent challenges and opportunities in gene therapy. Front Chem 2023;11:1259435. https://doi.org/10.3389/FCHEM.2023.1259435/XML/NLM.Search in Google Scholar
119. Mohite, P, Asane, G, Rebello, N, Munde, S, Ade, N, Boban, T, et al.. Polymeric hydrogel sponges for wound healing applications: a comprehensive review. Regen Eng Transl Med 2024 10 416–37. https://doi.org/10.1007/S40883-024-00334-4.Search in Google Scholar
120. Li, J, Chen, G, Xu, X, Abdou, P, Jiang, Q, Shi, D, et al.. Advances of injectable hydrogel-based scaffolds for cartilage regeneration; n.d. https://doi.org/10.1093/rb/rbz022.Search in Google Scholar PubMed PubMed Central
121. Goyal, R, Mahapatra, SN, Yadav, R, Mitra, S, Samanta, A, Kumar, A, et al.. 3D printed cellulose nanofiber-reinforced and iron-crosslinked double network hydrogel composites for tissue engineering applications: mechanical properties and cellular viability. Bioprinting 2025;46:e00392. https://doi.org/10.1016/J.BPRINT.2025.E00392.Search in Google Scholar
122. Najafinezhad, A, Nasiri-Harchegani, S, Drelich, JW, Arefi, R, Keshavarz, M, Bakhsheshi-Rad, HR. Polycaprolactone-chitosan-based scaffolds with nanostructured cuprorivaite fabricated via 3D printing for bone tissue engineering. Mater Lett 2025;397:138808. https://doi.org/10.1016/J.MATLET.2025.138808.Search in Google Scholar
123. Bashiri, Z, Khosrowpour, Z, Moghaddaszadeh, A, Jafari, D, Alizadeh, S, Nasiri, H, et al.. Optimizations of placenta extracellular matrix-loaded silk fibroin/alginate 3D-Printed scaffolds structurally and functionally for bone tissue engineering. Eng Life Sci 2025;25:e202400085. https://doi.org/10.1002/ELSC.202400085;CTYPE:STRING:JOURNAL.10.1002/elsc.202400085Search in Google Scholar PubMed PubMed Central
124. Aizarna-Lopetegui, U, Bittinger, SC, Álvarez, N, Henriksen-Lacey, M, Jimenez de Aberasturi, D. Stimuli-responsive hybrid materials for 4D in vitro tissue models. Mater Today Bio 2025;33:102035. https://doi.org/10.1016/J.MTBIO.2025.102035.Search in Google Scholar PubMed PubMed Central
125. Gan, D, Jiang, Y, Hu, Y, Wang, X, Wang, Q, Wang, K, et al.. Mussel-inspired extracellular matrix-mimicking hydrogel scaffold with high cell affinity and immunomodulation ability for growth factor-free cartilage regeneration. J Orthop Translat 2022;33:120–31. https://doi.org/10.1016/J.JOT.2022.02.006.Search in Google Scholar PubMed PubMed Central
126. Arora, A, Kothari, A, Katti, DS. Pore orientation mediated control of mechanical behavior of scaffolds and its application in cartilage-mimetic scaffold design. J Mech Behav Biomed Mater 2015;51:169–83. https://doi.org/10.1016/J.JMBBM.2015.06.033.Search in Google Scholar
127. Xing, X, Han, Y, Cheng, H. Biomedical applications of chitosan/silk fibroin composites: a review. Int J Biol Macromol 2023;240:124407. https://doi.org/10.1016/J.IJBIOMAC.2023.124407.Search in Google Scholar PubMed
128. Ferreira, ADBL, Nóvoa, PRO, Marques, AT. Multifunctional material systems: a state-of-the-art review. Compos Struct 2016;151:3–35. https://doi.org/10.1016/J.COMPSTRUCT.2016.01.028.Search in Google Scholar
129. Parisi, C, Salvatore, L, Veschini, L, Serra, MP, Hobbs, C, Madaghiele, M, et al.. Biomimetic gradient scaffold of collagen–hydroxyapatite for osteochondral regeneration. J Tissue Eng 2020;11:2041731419896068. https://doi.org/10.1177/2041731419896068.Search in Google Scholar PubMed PubMed Central
130. Ghosh, S, Pati, F. Decellularized extracellular matrix and silk fibroin-based hybrid biomaterials: a comprehensive review on fabrication techniques and tissue-specific applications. Int J Biol Macromol 2023;253. https://doi.org/10.1016/J.IJBIOMAC.2023.127410.Search in Google Scholar PubMed
131. Wu, J, Liu, J, Shi, Y, Wan, Y. Rheological, mechanical and degradable properties of injectable chitosan/silk fibroin/hydroxyapatite/glycerophosphate hydrogels. J Mech Behav Biomed Mater 2016;64:161–72. https://doi.org/10.1016/J.JMBBM.2016.07.007.Search in Google Scholar PubMed
132. Faheed, NK. Advantages of natural fiber composites for biomedical applications: a review of recent advances. Emergent Mater 2024;7:63–75. https://doi.org/10.1007/S42247-023-00620-X/FIGURES/3.Search in Google Scholar
133. Woods, A, Wang, G, Beier, F. Regulation of chondrocyte differentiation by the actin cytoskeleton and adhesive interactions. J Cell Physiol 2007;213:1–8. https://doi.org/10.1002/JCP.21110.Search in Google Scholar
134. Montaseri, Z, Abolmaali, SS, Tamaddon, AM, Farvadi, F. Composite silk fibroin hydrogel scaffolds for cartilage tissue regeneration. J Drug Deliv Sci Technol 2023;79. https://doi.org/10.1016/J.JDDST.2022.104018.Search in Google Scholar
135. Oreffo, ROC, Triffitt, JT. Future potentials for using osteogenic stem cells and biomaterials in orthopedics. Bone 1999;25. https://doi.org/10.1016/S8756-3282(99)00124-6.Search in Google Scholar PubMed
136. Murphy, CM, O’Brien, FJ, Little, DG, Schindeler, A. Cell-scaffold interactions in the bone tissue engineering triad. Eur Cell Mater 2013;26:120–32. https://doi.org/10.22203/ECM.V026A09.Search in Google Scholar PubMed
137. Kasravi, M, Ahmadi, A, Babajani, A, Mazloomnejad, R, Hatamnejad, MR, Shariatzadeh, S, et al., Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine, Biomater Res 2023 27:1–24. https://doi.org/10.1186/S40824-023-00348-Z.Search in Google Scholar PubMed PubMed Central
138. Longoni, A, Knežević, L, Schepers, K, Weinans, H, Rosenberg, AJWP, Gawlitta, D. The impact of immune response on endochondral bone regeneration. NPJ Regen Med 2018;3:22. https://doi.org/10.1038/S41536-018-0060-5.Search in Google Scholar PubMed PubMed Central
139. Yang, D, Xiao, J, Wang, B, Li, L, Kong, X, Liao, J. The immune reaction and degradation fate of scaffold in cartilage/bone tissue engineering. Mater Sci Eng C 2019;104. https://doi.org/10.1016/J.MSEC.2019.109927.Search in Google Scholar
140. Pandey, JK, Ahn, SH, Lee, CS, Mohanty, AK, Misra, M. Recent advances in the application of natural fiber based composites. Macromol Mater Eng 2010;295:975–89. https://doi.org/10.1002/MAME.201000095.Search in Google Scholar
141. Webber, MJ, Khan, OF, Sydlik, SA, Tang, BC, Langer, R. A perspective on the clinical translation of scaffolds for tissue engineering. Ann Biomed Eng 2014;43:641. https://doi.org/10.1007/S10439-014-1104-7.Search in Google Scholar PubMed PubMed Central
142. Ressler, A, Chitosan-based biomaterials for bone tissue engineering applications: a short review, Polymers 2022;14, Page 3430 14:3430. https://doi.org/10.3390/POLYM14163430.Search in Google Scholar PubMed PubMed Central
143. Jabbari, F, Akbari, B, Tayebi, L. Widely used biomaterials in cartilage biofabrication. In: Baghaban, Eslaminejad M, Hosseini, S, editors. Cartilage: from biology to biofabrication. Singapore: Springer; 2023:333–53 pp.10.1007/978-981-99-2452-3_12Search in Google Scholar
144. Bao, W, Li, M, Yang, Y, Wan, Y, Wang, X, Bi, N, et al.. Advancements and frontiers in the high performance of natural hydrogels for cartilage tissue engineering. Front Chem 2020;8:511126. https://doi.org/10.3389/FCHEM.2020.00053/PDF.Search in Google Scholar
145. Wu, X, Cheng, X, Kang, M, Dong, R, Zhao, J, Qu, Y. Natural polysaccharide-based hydrogel bioprinting for articular cartilage repair. Front Mater 2023;10:1204318. https://doi.org/10.3389/FMATS.2023.1204318/PDF.Search in Google Scholar
146. Tamayol, A, Akbari, M, Annabi, N, Paul, A, Khademhosseini, A, Juncker, D. Fiber-based tissue engineering: progress, challenges, and opportunities. Biotechnol Adv 2013;31:669–87. https://doi.org/10.1016/J.BIOTECHADV.2012.11.007.Search in Google Scholar PubMed PubMed Central
147. Golebiowska, AA, Tan, M, Ma, AWK, Nukavarapu, SP. Decellularized cartilage tissue bioink formulation for osteochondral graft development. Biomed Mater 2025;20:025002. https://doi.org/10.1088/1748-605X/ADA59D.Search in Google Scholar
148. Thomas, NG, Joy, S, Binci, PK, Jayachandran, VP, Prasad, PG, Jacob, S, et al.. Biomaterials from sustainable natural sources for tissue engineering. In: Application of engineering principles and practices in biotechnology. Palm Bay, FL: Apple Academic Press; 2024:43–86 pp.10.1201/9781003439929-5Search in Google Scholar
149. Zhang, Q, Wang, J, Chen, Z, al, -, Li, Y, Ma, J, et al.. Decellularized cartilage tissue bioink formulation for osteochondral graft development. Biomed Mater 2025;20:025002. https://doi.org/10.1088/1748-605X/ADA59D.Search in Google Scholar
150. Ding, Z, Cheng, W, Mia, MS, Lu, Q. Silk biomaterials for bone tissue engineering. Macromol Biosci 2021;21. https://doi.org/10.1002/MABI.202100153.Search in Google Scholar
151. Murali, VP, Jayakumar, R. Natural biopolymers in tissue engineering—role, challenges, and clinical applications. Natural Biopolymers in Drug Delivery and Tissue Engineering 2023:409–34. https://doi.org/10.1016/B978-0-323-98827-8.00020-5.Search in Google Scholar
152. Brittberg, M. Cellular and acellular approaches for cartilage repair: a philosophical analysis. Cartilage 2015;6:4S. https://doi.org/10.1177/1947603514536983.Search in Google Scholar PubMed PubMed Central
153. McCullen, SD, Autefage, H, Callanan, A, Gentleman, E, Stevens, MM. Anisotropic fibrous scaffolds for articular cartilage regeneration. Tissue Eng 2012;18:2073. https://doi.org/10.1089/TEN.TEA.2011.0606.Search in Google Scholar PubMed PubMed Central
154. Qiao, K, Xu, L, Tang, J, Wang, Q, Lim, KS, Hooper, G, et al., The advances in nanomedicine for bone and cartilage repair, J Nanobiotech 2022;20:1–42. https://doi.org/10.1186/S12951-022-01342-8.Search in Google Scholar
155. Primorac, D, Molnar, V, Tsoukas, D, Uzieliene, I, Tremolada, C, Brlek, P, et al.. Tissue engineering and future directions in regenerative medicine for knee cartilage repair: a comprehensive review. Croat Med J 2024;65:268. https://doi.org/10.3325/CMJ.2024.65.268.Search in Google Scholar
156. Takei, T, Kishihara, N, Ijima, H, Kawakami, K. Fabrication of capillary-like network in a matrix of water-soluble polymer using poly(methyl methacrylate) microfibers, artificial cells, blood substitutes, and. Biotechnology 2012;40:66–9. https://doi.org/10.3109/10731199.2011.592492.Search in Google Scholar PubMed
157. Miller, JS, Stevens, KR, Yang, MT, Baker, BM, Nguyen, DHT, Cohen, DM, et al.. Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues. Nat Mater 2012;11:768–74. ;SUBJMETA=301,54,639,990,994;KWRD=BIOMEDICAL+MATERIALS,TISSUES https://doi.org/10.1038/NMAT3357.Search in Google Scholar
158. Bégin-Drolet, A, Dussault, MA, Fernandez, SA, Larose-Dutil, J, Leask, RL, Hoesli, CA, et al.. Design of a 3D printer head for additive manufacturing of sugar glass for tissue engineering applications. Addit Manuf 2017;15:29–39. https://doi.org/10.1016/J.ADDMA.2017.03.006.Search in Google Scholar
159. Tamayol, A, Akbari, M, Annabi, N, Paul, A, Khademhosseini, A, Juncker, D. Fiber-based tissue engineering: progress, challenges, and opportunities. Biotechnol Adv 2012;31:669. https://doi.org/10.1016/J.BIOTECHADV.2012.11.007.Search in Google Scholar PubMed PubMed Central
160. Zhang, B, Huang, J, Narayan, RJ. Gradient scaffolds for osteochondral tissue engineering and regeneration. J Mater Chem B 2020;8:8149–70. https://doi.org/10.1039/D0TB00688B.Search in Google Scholar
161. Tan, G, Xu, J, Chirume, WM, Zhang, J, Zhang, H, Hu, X, Antibacterial and anti-inflammatory coating materials for orthopedic implants: a review, Coatings 2021;11, Page 1401 11:1401. https://doi.org/10.3390/COATINGS11111401.Search in Google Scholar
162. Zeng, S, Liu, L, Shi, Y, Qiu, J, Fang, W, Rong, M, et al.. Characterization of silk fibroin/chitosan 3D porous scaffold and in vitro cytology. PLoS One 2015;10:e0128658. https://doi.org/10.1371/JOURNAL.PONE.0128658.Search in Google Scholar
163. Wang, Z, Wang, Y, Yan, J, Zhang, K, Lin, F, Xiang, L, et al.. Pharmaceutical electrospinning and 3D printing scaffold design for bone regeneration. Adv Drug Deliv Rev 2021;174:504–34. https://doi.org/10.1016/J.ADDR.2021.05.007.Search in Google Scholar PubMed
164. Hartl, D, de Luca, V, Kostikova, A, Laramie, J, Kennedy, S, Ferrero, E, et al.. Translational precision medicine: an industry perspective. J Transl Med 2021;19. https://doi.org/10.1186/S12967-021-02910-6.Search in Google Scholar
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