Isolation, structural elucidation, and biological activity of a novel isocoumarin from the dark septate endophytic fungus Phialocephala fortinii
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Abstract
A novel isocoumarin was isolated from the mycelia of the dark septate endophytic fungus Phialocephala fortinii. The chemical structure was determined to be 8-hydroxy-6-methoxy-3,7-dimethyl-1H-2-benzopyran-1-one based on mass spectrometry, 1H-nuclear magnetic resonance (NMR), and 13C-NMR spectroscopic analyses, including 2D-NMR experiments. The isolated compound inhibited root growth of Arabidopsis thaliana, suggesting its potential as a plant growth regulator.
Funding source: Japan Society for the Promotion of Science
Award Identifier / Grant number: 20H04755
Funding source: Hokkaido University
Award Identifier / Grant number: Unassigned
Acknowledgments
We acknowledge the assistance of Dr. Eri Fukushi and Mr. Yusuke Takata (GC-MS &NMR Laboratory, Research Faculty of Agriculture, Hokkaido University) in obtaining the spectroscopic data. We thanks the ZEON Cooporation for providing methyl jasmonate, which is used as precursor of JA. We thank Editage [http://www.editage.com] for their English language service in editing and reviewing this manuscript.
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Research ethics: Not applicable.
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Author contributions: NK, YT, KN, and HM designed the experiments. KB performed all experiments under the technical guidance of RK, NK, and HM. All authors analyzed the data and wrote the manuscript. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: The authors state no conflicts of interest.
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Research funding: This work was supported by the Japan Society for the Promotion of Science (JSPS) (KAKENHI Grant Number 20H04755 to HM).
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Data availability: The raw data can be obtained on request from the corresponding author.
References
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Supplementary Material
This article contains supplementary material (https://doi.org/10.1515/znc-2023-0139).
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Artikel in diesem Heft
- Frontmatter
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- In silico studies on the anti-acne potential of Garcinia mangostana xanthones and benzophenones
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- Pyrane-based cembranoid and 2-dehydro-4-peroxy-sarcophine: two new diterpenes from Sarcophyton glaucum
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- Isolation, structural elucidation, and biological activity of a novel isocoumarin from the dark septate endophytic fungus Phialocephala fortinii
Artikel in diesem Heft
- Frontmatter
- Research Articles
- In silico studies on the anti-acne potential of Garcinia mangostana xanthones and benzophenones
- Novel 4-nitroimidazole analogues: synthesis, in vitro biological evaluation, in silico studies, and molecular dynamics simulation
- Dynamics of alkaloid accumulation in Narcissus cv. Hawera: a source of Sceletium-type alkaloids
- Pyrane-based cembranoid and 2-dehydro-4-peroxy-sarcophine: two new diterpenes from Sarcophyton glaucum
- Rapid Communication
- Isolation, structural elucidation, and biological activity of a novel isocoumarin from the dark septate endophytic fungus Phialocephala fortinii
