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Comparative study of phenolic profile, antioxidant and antimicrobial activities of aqueous extract of white and green tea

  • Gamal A. Gabr ORCID logo EMAIL logo , Hazem M.M. Hassan , Vidya D. Seshadri and Nahla M.M. Hassan
Published/Copyright: May 23, 2022
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Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 77 Issue 11-12

Abstract

The sole difference between white tea (WT) and green tea (GT) is the former that made only from the buds and young leaves of the Camelia sinensis plant, whilst the latter is made from matured tea leaves. The phytochemical profiles, phenolic compounds, antioxidant, and antimicrobial activity of two varieties of Camellia sinensis teas, white and green, were compared in this study. Total antioxidant capacity, reducing power, DPPH radical scavenging, and Fe+2 chelating activities were used to determine antioxidant activities in water extract of GT and WT. The largest level of phenolic content was discovered in WGTE compared with the lowest amount was found in WWTE (290.67 mg/100 g tea and 185.96 mg/100 g tea, respectively). Phenoilc acids (gallic, benzoic, chlorogenic, ellagic, and ρ-coumaric acids) and flavonoids (rutin and kampherol) were found in the two extracts. The findings of DPPH radical scavenging assays were 84.06 and 82.37% inhibition. In vitro antimicrobial activity was indicated that (WWTE and WGTE) had a high level of activity against Staphylococcus aureus, and gave negative activity against Salmonella typhimurium, and Aspergillus Niger. The WT and GT extracts are a great source of natural antioxidants with biological effects on human health.

Keywords: antimicrobial; antioxidant; Camellia sinensis ; herbal tea; HPLC; phenolic profile
Corresponding author: Gamal A. Gabr, Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj, 11942, Saudi Arabia; and Agricultural Genetic Engineering Research Institute, Agriculture Research Center, Giza, 12619, Egypt, E-mail:

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

References

1. Okaiyeto, K, Oguntibeju, OO. African herbal medicines: adverse effects and cytotoxic potentials with different therapeutic applications. Int J Environ Res Publ Health 2021;18:5988. https://doi.org/10.3390/ijerph18115988.Search in Google Scholar PubMed PubMed Central

2. Sirichaiwetchakoon, K, Lowe, GM, Eumkeb, G. The free radical scavenging and anti-isolated human LDL oxidation activities of Pluchea indica (L.) less. Tea compared to green tea (Camellia sinensis). BioMed Res Int 2020;2020:1–12. https://doi.org/10.1155/2020/4183643.Search in Google Scholar PubMed PubMed Central

3. Koo, SI, Noh, SK. Green tea as inhibitor of the intestinal absorption of lipids: potential mechanism for its lipid-lowering effect. J Nutr Biochem 2007;18:179–83. https://doi.org/10.1016/j.jnutbio.2006.12.005.Search in Google Scholar PubMed PubMed Central

4. Komes, D, Horži´c, D, Belšˇcak, A, Gani´c, KK, Vuli´c, I. Green tea preparation and its influence on the content of bioactive compounds. Food Res Int 2010;43:167–76.10.1016/j.foodres.2009.09.022Search in Google Scholar

5. Li, K, Shi, X, Yang, X, Wang, Y, Ye, C, Yang, Z. Antioxidative activities and the chemical constituents of two Chinese teas, Camellia kucha and C. ptilophylla. Int J Food Sci Technol 2012;47:1063–71. https://doi.org/10.1111/j.1365-2621.2012.02942.x.Search in Google Scholar

6. Bhardwaj, P, Kumar, R, Sharma, H, Tewari, R, Ahuja, PS, Sharma, RK. Development and utilization of genomic and genic microsatellite markers in Assam tea (Camellia assamica ssp. assamica) and related Camellia species. Plant Breed 2013;132:748–63. https://doi.org/10.1111/pbr.12101.Search in Google Scholar

7. Naveed, M, BiBi, J, Kamboh, AA, Suheryani, I, Kakar, I, Fazlani, SA, et al.. Pharmacological values and therapeutic properties of black tea (Camellia sinensis): a comprehensive overview. Biomed Pharmacother 2018;100:521–31. https://doi.org/10.1016/j.biopha.2018.02.048.Search in Google Scholar PubMed

8. Sharangi, AB. Medicinal and therapeutic potentialities of tea (Camellia sinensis L.) A review. Food Res Int 2009;42:529–35. https://doi.org/10.1016/j.foodres.2009.01.007.Search in Google Scholar

9. Xing, L, Zhang, H, Qi, R, Tsao, R, Mine, Y. Recent advances in the understanding of the health benefits and molecular mechanisms associated with green tea polyphenols. J Agric Food Chem 2019;67:1029–43. https://doi.org/10.1021/acs.jafc.8b06146.Search in Google Scholar PubMed

10. Khan, N, Mukhtar, H. Tea polyphenols for health promotion. Life Sci 2007;81:519–33. https://doi.org/10.1016/j.lfs.2007.06.011.Search in Google Scholar PubMed PubMed Central

11. Bakht, MA, Geesi, MH, Riadi, Y, Imran, M, Ali, MI, Ahsan, MJ, et al.. Ultrasound-assisted extraction of some branded tea: optimization based on polyphenol content, antioxidant potential and thermodynamic study. Saudi J Biol Sci 2019;26:1043–52. https://doi.org/10.1016/j.sjbs.2018.07.013.Search in Google Scholar

12. Kmiecik, D, Gramza-Michałowska, A, Korczak, J. Anti-polymerization activity of tea and fruits extracts during rapeseed oil heating. Food Chem 2018;239:858–64. https://doi.org/10.1016/j.foodchem.2017.07.025.Search in Google Scholar

13. Adnan, M, Ahmad, A, Ahmed, A, Khalid, N, Hayat, I, Ahmed, I. Chemical composition and sensory evaluation of tea (Camellia sinensis) commercialized in Pakistan. Pakistan J Bot 2013;45:901–7.Search in Google Scholar

14. Huo, C, Dou, QP, Chan, TH. Synthesis of phosphates and phosphates-acetates hybrids of green tea polyphenol (-)- epigallocatechine-3-gallate (EGCG) and its G ring deoxy analogs as potential anticancer prodrugs. Tetrahedron Lett 2011;52:5478–83. https://doi.org/10.1016/j.tetlet.2011.08.061.Search in Google Scholar

15. Lorenzo, JM, Munekata, PES. Phenolic compounds of green tea: health benefits and technological application in food. Asian Pac J Trop Biomed 2016;6:709–19. https://doi.org/10.1016/j.apjtb.2016.06.010.Search in Google Scholar

16. Kellogg, JJ, Graf, TN, Paine, MF, McCune, JS, Kvalheim, OM, Oberlies, NH, et al.. Comparison of metabolomics approaches for evaluating the variability of complex botanical preparations: green tea (Camellia sinensis) as a case study. J Nat Prod 2017;80:1457–66. https://doi.org/10.1021/acs.jnatprod.6b01156.Search in Google Scholar

17. Yang, C, Hu, Z, Lu, M, Li, P, Tan, J, Chen, M, et al.. Application of metabolomics profiling in the analysis of metabolites and taste quality in different subtypes of white tea. Food Res Int 2018;106:909–19. https://doi.org/10.1016/j.foodres.2018.01.069.Search in Google Scholar

18. Senger, AE, Schwanke, CH, Gottlieb, MG. Green tea (Camellia sinensis) and its functional properties on transmissible chronic diseases [in English]. Sci Med 2011;20:292–300.Search in Google Scholar

19. Del Rio, D, Rodriguez-Mateos, A, Spencer, JPE, Tognolini, M, Borges, G, Crozier, A. Dietary (Poly)phenolics in human health: structures, bioavailability, and evidence of protective effects against chronic diseases. Antioxidants Redox Signal 2013;18:1818–92. https://doi.org/10.1089/ars.2012.4581.Search in Google Scholar

20. Santana-Rios, G, Orner, GA, Amantana, A, Provost, C, Wu, SY, Dashwood, RH. Potent antimutagenic activity of white tea in comparison with green tea in the Salmonella assay. Mutat Res 2001;495:61–74. https://doi.org/10.1016/s1383-5718(01)00200-5.Search in Google Scholar

21. Yang, H, Xue, X, Li, H, Apandi, SN, Tay-chan, SC, Ong, SP, et al.. The relative antioxidant activity and steric structure of green tea catechins–A kinetic approach. Food Chem 2018;257:399–405. https://doi.org/10.1016/j.foodchem.2018.03.043.Search in Google Scholar PubMed

22. Espinosa, C, López-Jiménez, JA, Pérez-Llamas, F, Guardiola, FA, Esteban, MA, Arnao, MB, et al.. Long-term intake of white tea prevents oxidative damage caused by adriamycin in kidney of rats. J Sci Food Agric 2016;96:3079–87. https://doi.org/10.1002/jsfa.7483.Search in Google Scholar PubMed

23. Hadi, A, Pourmasoumi, M, Kafeshani, M, Karimian, J, Maracy, MR, Entezari, MH. The effect of green tea and sour tea (Hibiscus sabdariffa L.) supplementation on oxidative stress and muscle damage in athletes. J Diet Suppl 2017;14:346–57. https://doi.org/10.1080/19390211.2016.1237400.Search in Google Scholar PubMed

24. Idu, AS, Igeleke, J. Preliminary phytochemical analysis of some plant seeds. Res J Chem Sci 2012;1:58–62.Search in Google Scholar

25. Harbone, JB Phytochemical methods. In: Stamer, JS, editor Nirosative stress. Method in enzymology, 3rd ed. London: Chapman & Hall; 1998, vol 300. p. 389.Search in Google Scholar

26. Astill, C, Mark, RB, Clive, D, Philip, GH, Philip, TM. Factors affecting the caffeine and polyphenol contents of black and green tea infusions. J Agric Food Chem 2001;49:5340–7. https://doi.org/10.1021/jf010759+.10.1021/jf010759+Search in Google Scholar PubMed

27. Singh, RP, Murthy, KNC, Jayaprakasha, GK. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J Agric Food Chem 2002;50:81–6. https://doi.org/10.1021/jf010865b.Search in Google Scholar PubMed

28. Kumaran, A, Karunakaran, RJ. In vitro antioxidant activities of methanol extracts of five phyllanthus species from India. LWT – Food Sci Technol 2007;40:344–52. https://doi.org/10.1016/j.lwt.2005.09.011.Search in Google Scholar

29. Mattila, P, Astola, J, Kumpulainen, J. Determination of flavonoids in plant material by HPLC with diode array and electro array detections. J Agric Food Chem 2000;48:5834–41. https://doi.org/10.1021/jf000661f.Search in Google Scholar PubMed

30. Mathew, S, Abraham, TE. Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models. Food Chem 2006;94:520–8. https://doi.org/10.1016/j.foodchem.2004.11.043.Search in Google Scholar

31. Lee, JY, Hwang, WI, Lim, ST. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J Ethnopharmacol 2004;93:409–15. https://doi.org/10.1016/j.jep.2004.04.017.Search in Google Scholar PubMed

32. Oboh, G, Puntel, RL, Rocha, JBT. Hot pepper (Capsicum annuum, Tepin and Capsicum Chinese, Habanero) prevents Fe2+-induced lipid peroxidation in brain in vitro. Food Chem 2007;102:178–85. https://doi.org/10.1016/j.foodchem.2006.05.048.Search in Google Scholar

33. Valgas, C, De Souza, SM, Smânia, EFA. Screening methods to determine antibacterial activity of natural products. Braz J Microbiol 2007;38:369–80. https://doi.org/10.1590/s1517-83822007000200034.Search in Google Scholar

34. Duncan, DB. Multiple range and multiple F tests. Biometrics 1955;11:1–41. https://doi.org/10.2307/3001478.Search in Google Scholar

35. Sashi, KJ, Ramya, M, Janardhan, K. Antimicrobial activity of ethnomedicinal plants of Nilgiri biosphere reserve and Western Ghats. Asian J Microbiol Biotechnol Environ Sci 2003;5:183–5.Search in Google Scholar

36. Soliman, SM, Semreen, MH, El-Keblawy, AA. Assessment of herbal drugs for promising anti-Candida activity. BMC Compl. Med. Ther. 2017;17:257. https://doi.org/10.1186/s12906-017-1760-x.Search in Google Scholar PubMed PubMed Central

37. Rusak, G, Komes, D, Likic, S, Horzic, D, Kovac, M. Phenolic content and antioxidative capacity of green and white tea extracts depending on extraction conditions and the solvent used. Food Chem 2008;110:852858. https://doi.org/10.1016/j.foodchem.2008.02.072.Search in Google Scholar PubMed

38. Almajano, MP, Carbo, R, Jimenez, JA, Gordon, MH. Antioxidant and antimicrobial activities of tea infusions. Food Chem 2008;108:55–63. https://doi.org/10.1016/j.foodchem.2007.10.040.Search in Google Scholar

39. Unachukwu, UJ, Ahmed, S, Kavalier, A, Lyles, JT, Kennelly, EJ. White and green teas (Camellia sinensis var. sinensis): variation in phenolic, methylxanthine, and antioxidant profiles. J Food Sci 2010;75:541–8. https://doi.org/10.1111/j.1750-3841.2010.01705.x.Search in Google Scholar PubMed

40. Ürak, HH, Yagar, H, Isbilir, SS, Demirci, AS, Gumus, T. Antioxidant and antimicrobial activities of white, green, and black tea extracts. Acta Aliment 2013;42:379–89.10.1556/AAlim.2013.2222Search in Google Scholar

41. Okwu, DE. Phytochemicals and vitamin content of indigenous spices of south eastern Nigeria. J Sustain Agric Environ 2004;6:30–7.Search in Google Scholar

42. Horzic, D, Komes, D, Belscak, A, Ganic, KK, Ivekovic, D, Karlovic, D. The composition of polyphenols and methylxanthines in teas and herbal infusions. Food Chem 2009;115:441–8.10.1016/j.foodchem.2008.12.022Search in Google Scholar

43. Croft, KC. Antioxidant effects of plant phenolic compounds. Oxford, UK: CAB International Publishing Inc; 1999. pp. 109–21.Search in Google Scholar

44. Anesini, C, Graciela, EF, Rosana, F. Total polyphenol content and antioxidant capacity of commercially available tea (Camellia sinensis) in Argentina. J Agric Food Chem 2008;56:9225–9. https://doi.org/10.1021/jf8022782.Search in Google Scholar PubMed

45. Shrestha, R, Lama, JP, Shrestha, K. Total polyphenols content and antioxidant activity of different tea commercially produced in Nepal. J Food Sci Technol Nepal 2010;6:73–9.10.3126/jfstn.v6i0.8264Search in Google Scholar

46. Hashish, AS. Effect of tea leaves on reduction of potato chips Hazards. Egypt J Nutr 2016;31:21–47.Search in Google Scholar

47. Suteerapataranon, S, Pudta, D. Flow injection analysis-spectrophotometry for rapid determination of total polyphenols in tea extracts. J Flow Inject Anal 2008;25:61–4.Search in Google Scholar

48. Higdon, JV, Frei, B. Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions. Crit Rev Food Sci Nutr 2003;43:89–143. https://doi.org/10.1080/10408690390826464.Search in Google Scholar PubMed

49. Yuan, Y, Chen, C, Yang, B, Kusu, F, Kotani, A. DPPH radical scavenging activities of 31flavonoids and phenolic acids and 10 extracts of Chinese materia medica. China J Chin Mater Med 2009;34:1695–700.Search in Google Scholar

50. Honzel, D, Carter, SG, Redman, KA, Schauss, AG, Endres, JR, Jensen, GS. Comparison of chemical and cell-based antioxidant methods for evaluation of foods and natural products: generating multifaceted data by parallel testing using erythrocytes and polymorphonuclear cells. J Agric Food Chem 2008;56:8319–25. https://doi.org/10.1021/jf800401d.Search in Google Scholar PubMed

51. Rohan, A, Navid, I, Kiran, M, Saqlain, J, Ali, A, Muhmmad, L, et al.. Determination of antibacterial activity of tea water concentrates against E. coli and S. aureus. Pure Appl Biol 2021;10:962–8.10.19045/bspab.2021.100100Search in Google Scholar

52. Steinmann, J, Buer, J, Pietschmann, T, Steinmann, E. Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea. Br J Pharmacol 2013;168:1059–73. https://doi.org/10.1111/bph.12009.Search in Google Scholar PubMed PubMed Central

53. Dias, T, Tomás, G, Teixeira, N. White tea (Camellia sinensis (L.)): antioxidant properties and beneficial health effects. Int J Food Sci Nutr Diet 2013;2:19–26.Search in Google Scholar

54. Thring, TSA, Hili, P, Naughton, DP. Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Compl Alternative Med 2009;9:27–36. https://doi.org/10.1186/1472-6882-9-27.Search in Google Scholar PubMed PubMed Central

55. Elmastasa, M, Isildaka, O, Turkekulb, I, Temura, N. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J Food Compos Anal 2007;20:337–45. https://doi.org/10.1016/j.jfca.2006.07.003.Search in Google Scholar

56. Gordon, MH. The mechanism of antioxidant action in vitro. In: Hudson, BJF, editor. Food antioxidants. London, UK: Elsevier Publishing Inc; 1990. p. 1–15.10.1007/978-94-009-0753-9_1Search in Google Scholar

57. Zhu, WF, Wang, CL, Ye, F, Sun, HP, Ma, CY, Liu, WY, et al.. Chemical constituents of the seed cake of Camellia oleifera and their antioxidant and antimelanogenic activities. Chem Biodivers 2018;15:1800137. https://doi.org/10.1002/cbdv.201800137.Search in Google Scholar PubMed

58. Luo, F, Fei, X. Distribution and antioxidant activities of free, conjugated, and insoluble-bound phenolics from seven species of the genus Camellia. J Am Oil Chem Soc 2018;96:159–70. https://doi.org/10.1002/aocs.12172.Search in Google Scholar

59. Nibir, YM, Sumit, AF, Akhand, AA, Ahsan, N, Hossain, MS. Comparative assessment of total polyphenols, antioxidant and antimicrobial activity of different tea varieties of Bangladesh. Asian Pac J Trop Biomed 2017;7:352–7. https://doi.org/10.1016/j.apjtb.2017.01.005.Search in Google Scholar

60. Charkraborty, D, Charkraborti, S. Bioassay-guided isolation and identification of antibacterial and antifungal component from methanolic extract of green tea leaves (Camellia sinensis). Res. J Phythochem 2010;4:78–86.10.3923/rjphyto.2010.78.86Search in Google Scholar
Received: 2021-12-19
Accepted: 2022-04-30
Published Online: 2022-05-23
Published in Print: 2022-11-25

© 2022 Walter de Gruyter GmbH, Berlin/Boston

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  2. Research Articles
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https://doi.org/10.1515/znc-2021-0321
Keywords for this article
antimicrobial; antioxidant; Camellia sinensis; herbal tea; HPLC; phenolic profile

Articles in the same Issue

  1. Frontmatter
  2. Research Articles
  3. Synthesis, characterization, molecular docking, dynamics simulations, and in silico absorption, distribution, metabolism, and excretion (ADME) studies of new thiazolylhydrazone derivatives as butyrylcholinesterase inhibitors
  4. Study on the synthesis and structure-activity relationship of 1,2,3-triazoles against toxic activities of Bothrops jararaca venom
  5. In-silico elucidation reveals potential phytochemicals against angiotensin-converting enzyme 2 (ACE-2) receptor to fight coronavirus disease 2019 (COVID-19)
  6. Comparative study of phenolic profile, antioxidant and antimicrobial activities of aqueous extract of white and green tea
  7. Methylglyoxal and high glucose inhibit VEGFR2 phosphorylation at specific tyrosine residues
  8. Antidepressant alkaloids from the rhizomes of Corydalis decumbens
  9. Synthesis of new derivatives containing pyridine, investigation of MAO inhibitory activities and molecular docking studies
  10. Rapid Communications
  11. Chemical composition and bioactivities of Magnolia candollii H.Keng essential oil
  12. Chemical composition and anticholinesterase activity of Lepisanthes rubiginosa (Roxb.) Leenh. essential oil
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