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Impact of gamma irradiation on phytochemical composition, and biological activities of Lepidium sativum seeds extract

  • Samia Hadj Rabia EMAIL logo , Aicha Debib , Atika Eddaikra , Lila Aberkane-Mchebbek , Ramy Nouri , Fatima Benmoussa , Amine Mokhtari , Sarah Medjber , Belkacem Mansouri and Mohammed Messaoudi ORCID logo EMAIL logo
Published/Copyright: March 29, 2024
Radiochimica Acta
From the journal Radiochimica Acta Volume 112 Issue 5

Abstract

The main objective of this research is to assess how gamma radiation influences the chemical composition and activities such as antioxidant, antimicrobial and anticancer activities of Lepidium sativum (L. sativum) seeds. Methanolic extracts were obtained through a 24 h maceration process from L. sativum seeds exposed to various doses of gamma irradiation. Phytochemical screening involved precipitation and staining reactions, with the antioxidant potential assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Antimicrobial activity was evaluated through a diffusion test on agar medium, while the cytotoxic potential on the lung cancer line “A-549 cells” was examined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Qualitative analysis of the extracts identified preserved bioactive constituents after irradiation. Additionally, quantitative analysis indicated a significant rise in the total content of polyphenols and flavonoids under radiation, accompanied by an enhanced reducing power compared to the control. The antimicrobial and cytotoxic capabilities also improved, evident in increased growth inhibition zones for certain microbial strains and reduced viability rates of A549 cells. These results show promise and warrant further investigation into the potential of gamma radiation to enhance additional biological effects of plants.

Keywords: gamma radiation; Lepidium sativum ; antioxidant activity; antimicrobial activity; cytotoxic activity; chemical composition
Corresponding authors: Samia Hadj Rabia, Department of Nuclear Applications, Nuclear Research Center of Draria, B.P.43 Sebala-Draria, Algeria, E-mail: ; and Mohammed Messaoudi, Nuclear Research Centre of Birine, P.O. Box 180, Ain Oussera, Djelfa 17200, Algeria, E-mail:

  1. Research ethics: Not applicable.

  2. Author contributions: SH contributed to designing, interpretation of data and drafting the manuscript. AD have been involved in performing experiments and revising the manuscript. LA, SH, RN, FB, AM, SM, MM contributed to the experiments and data collection, AE contributed to the statistical analysis, BM provided a technical contribution to the realization of irradiation. All authors have read and agreed to the published version of the manuscript.

  3. Conflicting interest: All authors declare no conflict of interest.

  4. Research funding: None declared.

  5. Data availability: Not applicable.

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Received: 2023-11-27
Accepted: 2024-03-13
Published Online: 2024-03-29
Published in Print: 2024-05-27

© 2024 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Original Papers
  3. Speciation simulation and sorption mechanism of 238Pu in radioactive waste repository lithosphere
  4. Magnetic alginate supported potassium manganese ferrocyanide for the recovery of uranium from acidic wastewater
  5. Separation of ReO4−/TcO4− from simulated radioactive waste liquid by a novel series of anion exchange resins
  6. Technetium-99m radiolabeling through conjugation with l,l-ethylene dicysteine chelator of a trimethoxylated flavone and its bioevaluation in rat with induced C6 glioma tumor as a new cancer diagnostic agent
  7. The effect of gamma rays and stearic acid on calcium carbonate and its impact on the properties of epoxy-based composites
  8. Impact of gamma irradiation on phytochemical composition, and biological activities of Lepidium sativum seeds extract
https://doi.org/10.1515/ract-2023-0260
Keywords for this article
gamma radiation; Lepidium sativum; antioxidant activity; antimicrobial activity; cytotoxic activity; chemical composition

Articles in the same Issue

  1. Frontmatter
  2. Original Papers
  3. Speciation simulation and sorption mechanism of 238Pu in radioactive waste repository lithosphere
  4. Magnetic alginate supported potassium manganese ferrocyanide for the recovery of uranium from acidic wastewater
  5. Separation of ReO4−/TcO4− from simulated radioactive waste liquid by a novel series of anion exchange resins
  6. Technetium-99m radiolabeling through conjugation with l,l-ethylene dicysteine chelator of a trimethoxylated flavone and its bioevaluation in rat with induced C6 glioma tumor as a new cancer diagnostic agent
  7. The effect of gamma rays and stearic acid on calcium carbonate and its impact on the properties of epoxy-based composites
  8. Impact of gamma irradiation on phytochemical composition, and biological activities of Lepidium sativum seeds extract
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