Phytochemical and antioxidant activity of Cadaba farinosa Forssk stem bark extracts
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Emmanuel M. Halilu
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Abdullahi M. Abdurrahman
Abstract
Cadaba farinosa is used in traditional medicine for treatment of cancer, diabetes, and rheumatism. The research was aimed at evaluating the phytochemical and antioxidant activity of the extracts. The powdered stem bark was extracted successively with the aid of Soxhlet extractor using n-hexane, ethyl acetate, and methanol. The resulting extracts were concentrated on rotary evaporator and the percentage yields were calculated. The phytochemical and TLC profiles of the extracts were studied. The antioxidant activity of the extracts and ascorbic acid (standard) were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay. The total phenolic content of the extracts and tannic acid (standard) were evaluated using Folin–Ciocalteu reagent. The percentage yields of n-hexane, ethyl acetate, and methanol extracts were found to be 1.19, 1.37, and 13.93%, respectively. The phytochemical screening revealed the presence of tannins, flavonoids, saponins, cardiac glycosides, alkaloids, and triterpenoids. The TLC profiles of the extracts revealed the presence of compounds as evidenced from their R f values. The total phenolic content of ethyl acetate and methanol extracts were found to be 135 and 112 mg, respectively. The free radical scavenging activity demonstrated by the extracts was comparable to ascorbic acid. The ethyl acetate extract had higher phenolic content and demonstrated the highest free radical scavenging with IC50 31.07 mg/mL. The results of research have provided strong preliminary evidence of antioxidant activity which may be because of phenolic compounds in the extracts.
Acknowledgements
The authors express their sincere appreciation to Mr. Aliyu A. Garba of the laboratory of the Department of Pharmacognosy and Ethnomedicine for the assistance in running the phytochemical screening and the determination of the antioxidant activity.
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: None declared.
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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Articles in the same Issue
- Frontmatter
- Reviews
- Evaluation of the Chemistry curriculum at the lower secondary level: feedback from educators
- Polymers, plastics, & more – educating post-secondary students from different disciplines with polymer science
- Quadrupole inductively coupled plasma mass spectrometry and sector field ICP-MS: a comparison of analytical methods for the quantification of As, Pb, Cu, Cd, Zn, and U in drinking water
- Development of reverse phase-high performance liquid chromatography (RP-HPLC) method for determination of selected antihypertensive active flavonoids (rutin, myricetin, quercetin, and kaempferol) in medicinal plants found in Botswana
- Accumulation and health effects of metals in selected urban groundwater
- Phytochemical and antioxidant activity of Cadaba farinosa Forssk stem bark extracts
Articles in the same Issue
- Frontmatter
- Reviews
- Evaluation of the Chemistry curriculum at the lower secondary level: feedback from educators
- Polymers, plastics, & more – educating post-secondary students from different disciplines with polymer science
- Quadrupole inductively coupled plasma mass spectrometry and sector field ICP-MS: a comparison of analytical methods for the quantification of As, Pb, Cu, Cd, Zn, and U in drinking water
- Development of reverse phase-high performance liquid chromatography (RP-HPLC) method for determination of selected antihypertensive active flavonoids (rutin, myricetin, quercetin, and kaempferol) in medicinal plants found in Botswana
- Accumulation and health effects of metals in selected urban groundwater
- Phytochemical and antioxidant activity of Cadaba farinosa Forssk stem bark extracts
