Taxonomic evaluation of the bent-winged bat (Miniopterus) populations occurring in Iran inferred from mitochondrial cytochrome-b sequences
Abstract
We studied the genetic differentiation and taxonomy of the bent-wing bat (Miniopterus) populations occurring in Iran by analyzing the mitochondrial cytochrome-b (687 bp) sequences. Molecular data provided by the present study exhibit a closer average K2P genetic distance among the Miniopterus populations in Iran and Azerbaijan (0.05) than that among those in Iran and Turkey (1.38), Iran and Spain (2.93) and Iran and Georgia (3.16). Moreover, the phylogenetic trees showed that all Iranian and Azerbaijani samples, as well as those from East Turkey, are grouped in the same clade, whereas the samples from other parts of Turkey, Spain and Georgia belong to another clade. These results suggest that the Miniopterus populations in Iran and Azerbaijan, together with those from East Turkey, are in fact Miniopterus pallidus, whereas the other Turkish samples, together with those from Georgia and western Europe, belong to Miniopterus schreibersii.
Funding statement: Funding: This study was supported by Razi University as a MSc research project for the second author.
References
Akmali, V., A. Farazmand, J. Darvish and M. Sharifi. 2011. Phylogeography and taxonomic status of the greater mouse-tailed bat Rhinopoma microphyllum (Chiroptera: Rhinopomatidae) in Iran. Acta Chiropterol. 13: 279–290.10.3161/150811011X624767Search in Google Scholar
Albayrak, I. and S. Coskun. 2000. Geographic variations and taxonomic status of Miniopterus schreibersi (Kuhl, 1819) in Turkey (Chiroptera: Vespertilionidae). Turk. J. Zool. 24: 125–134.Search in Google Scholar
Appleton, B.R., J.A. McKenzie and L. Christidis. 2004. Molecular systematics and biogeography of the bent-wing bat complex Miniopterus schreibersii (Kuhl, 1817) (Chiroptera: Vespertilionidae). Mol. Phylogenet. Evol. 31: 431–439.Search in Google Scholar
Baker, R.J. and R.D. Bradley. 2006. Speciation in mammals and the genetic species concept. J. Mammal. 87: 643–662.10.1644/06-MAMM-F-038R2.1Search in Google Scholar PubMed PubMed Central
Barratt, E.M., R. Deaville, T.M. Burland, M.W. Bruford, G. Jones, P.A. Racey and R.K. Wayne. 1997. DNA answers the call of pipistrelle bat species. Nature 387: 138–139.10.1038/387138b0Search in Google Scholar PubMed
Benda, P., M. Andreas, D. Kock, R.K. Lučan, P. Munclinger, P. Nová, J. Obuch, K. Ochman, A. Reiter, M. Uhrin and D. Weinfurtová. 2006. Bats (Mammalia: Chiroptera) of the Eastern Mediterranean. Part 4. Bat fauna of Syria: distribution, systematics, ecology. Acta Soc. Zool. Bohem. 70: 1–329.Search in Google Scholar
Bilgin, R., A. Karataş, E. Çoraman, I. Pandurski, E. Papadatou and J.C. Morales. 2006. Molecular taxonomy and phylogeography of Miniopterus schreibersii (Kuhl, 1817) (Chiroptera: Vespertilionidae) in the Eurasian transition. Biol. J. Linn. Soc. 87: 577–582.Search in Google Scholar
Bilgin, R., A. Karataş, E. Çoraman, T. Disotell and J.C. Morales. 2008. Regionally and climatically restricted patterns of distribution of genetic diversity in a migratory bat species, Miniopterus schreibersii (Chiroptera: Vespertilionidae). BMC Evol. Biol. 8: 209.Search in Google Scholar
Bilgin, R., K. Gürün, Ö. Maraci, A. Furman, P. Hulva, E. Çoraman, R.K. Lučan, T Bartonička and I. Horáček. 2012. Syntopic occurrence in Turkey supports separate species status for Miniopterus schreibersii schreibersii and M. schreibersii pallidus (Mammalia: Chiroptera). Acta Chiropterol. 14: 279–289.10.3161/150811012X661611Search in Google Scholar
Castella, V., M. Ruedi, L. Excoffier, C. Ibáñez, R. Arlettaz and J. Hausser. 2000. Is the Gibraltar Strait a barrier to gene flow for the bat Myotis myotis (Chiroptera: Vespertilionidae)? Mol. Ecol. 9: 1761–1772.10.1046/j.1365-294x.2000.01069.xSearch in Google Scholar PubMed
Cooper, S.J.B., P.R. Day, T.B. Reardon and M. Schulz. 2001. Assessment on species boundaries in Australian Myotis (Chiroptera: Vespertilionidae) using mitochondrial DNA. J. Mammal. 82: 328–338.10.1093/jmammal/82.2.328Search in Google Scholar
Corbet, G.B. 1978. The mammals of the Palaearctic region: a taxonomic review. Cornell University Press, Ithaca, NY. pp. 314.Search in Google Scholar
DeBlase, A.T. 1980. The bats of Iran: systematics, distribution, ecology. Field. Zool. 4: 1–424.Search in Google Scholar
Ellerman, J.R. and T.C.S. Morrison-Scott. 1951. Checklist of Palaearctic and Indian Mammals 1758 to 1946. British Museum (Natural History) London. pp. 810.Search in Google Scholar
Furman, A., E. Çoraman, R. Bilgin and A. Karataş. 2009. Molecular ecology and phylogeography of the bent-wing bat complex (Miniopterus schreibersii) (Chiroptera: Vespertilionidae) in Asia Minor and adjacent regions. Zool. Scr. 38: 129–141.10.1111/j.1463-6409.2008.00365.xSearch in Google Scholar
Furman, A., T. Öztunç and E. Çoraman. 2010a. On the phylogeny of Miniopterusschreibersiischreibersii and Miniopterusschreibersiipallidus from Asia Minor in reference to other Miniopterus taxa (Chiroptera: Vespertilionidae). Acta Chiropterol. 12: 61–72.10.3161/150811010X504590Search in Google Scholar
Furman, A., T. Postawa, T. Öztunc and E. Çoraman. 2010b. Cryptic diversity of the bent-wing bat, Miniopterus schreibersii (Chiroptera: Vespertilionidae), in Asia Minor. BMC Evol. Biol. 10: 121.Search in Google Scholar
Goodman, S.M., C.P. Maminirina, H.M. Bradman, L. Christidis and B. Appleton. 2009. The use of molecular phylogenetic and morphological tools to identify cryptic and paraphyletic species: examples from the diminutive long-fingered bats (Chiroptera: Miniopteridae: Miniopterus) on Madagascar. Am. Mus. Novit. 3669: 1–34.10.1206/652.1Search in Google Scholar
Guindon, S. and O. Gascuel. 2003. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 52: 696–704.Search in Google Scholar
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Res. 4: 95–98.Search in Google Scholar
Harrison, D.L. 1956. The mammals of Arabia. 1. Insectivores; Chiroptera; Primates. Ernest Benn Ltd., London, UK. pp. 192.Search in Google Scholar
Hoffmann, F.G. and R.J. Baker. 2003. Comparative phylogeography of short-tailed bats (Carollia: Phyllostomidae). Mol. Ecol. 12: 3403–3414.10.1046/j.1365-294X.2003.02009.xSearch in Google Scholar
Irwin, D.M., T.D. Kocher and A.C. Wilson. 1991. Evolution of the cytochrome b gene of mammals. J. Mol. Evol. 32: 128–144.10.1007/BF02515385Search in Google Scholar
Johns, G.C. and J.C. Avise. 1998. A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Mol. Biol. Evol 15: 1481–1490.10.1093/oxfordjournals.molbev.a025875Search in Google Scholar
Karami, M., R. Hutterer, P. Benda, R. Siahsarvie and B. Kryštufek. 2008. Annotated check-list of the mammals of Iran. Lynx (Praha), n. s. 39: 63–102.Search in Google Scholar
Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111–120.10.1007/BF01731581Search in Google Scholar
Koopman, K.F. 1993. Order Chiroptera. In: (D.E. Wilson and D.M. Reeder, eds.) Mammal species of the world: a taxonomic and geographic reference. Second edition. Smithsonian Institution, Washington, DC. pp. 1206.Search in Google Scholar
Koopman, K.F. 1994. Chiroptera: systematics. Handbook of zoology, Vol. 8. Mammalia. Walter de Gruyter, New York, NJ. pp. 217.Search in Google Scholar
Kuhl, H. 1817. Die Deutschen Fledermäuse. Privately published, Hanau, Germany. pp. 67.Search in Google Scholar
Lay, D.M. 1967. A study of the mammals of Iran resulting from the Street expedition of 1962–63. Field. Zool. 54: 1–282.10.5962/bhl.title.2961Search in Google Scholar
Maeda, K. 1982. Studies on the classification of Miniopterus in Eurasia, Australia and Melanesia. Honyurui Kagaku (Mammal. Sci.) Suppl. 1: 1–176.Search in Google Scholar
Miller-Butterworth, C.M., G. Eick, D.S. Jacobs, M.C. Schoeman and E.H. Harley. 2005. Genetic and phenotypic differences between South African long-fingered bats, with a global miniopterine phylogeny. J. Mammal. 86: 1121–1135.Search in Google Scholar
Pereira, M.J., P. Salgueiro, L. Rodrigues, M.M. Coelho and J.M. Palmeirim. 2009. Population structure of a cave dwelling bat, Miniopterus schreibersii: does it reflect history and social organization? J. Hered. 100: 533–544.Search in Google Scholar
Petit, E. and F. Mayer. 1999. Male dispersal in the noctule bat (Nyctalus noctula): where are the limits? Proc. R. Soc. Lond. Ser. B Biol. 266: 1717–1722.10.1098/rspb.1999.0837Search in Google Scholar
Posada, D. 2008. JModelTest: Phylogenetic model averaging. Mol. Biol. Evol. 25: 1253–1256.10.1093/molbev/msn083Search in Google Scholar
Rambaut, A. and A.J. Drummond. 2009. Tracer v1.5.0. Available from http://beast.bio.ed.ac.uk/Trace. (Accessed on 23 April 2011).Search in Google Scholar
Rodriguez, R.M. and L.K. Ammerman. 2004. Mitochondrial DNA divergence does not reflect morphological difference between Myotis californicus and Myotis ciliolabrum. J. Mammal. 85: 842–851.10.1644/1545-1542(2004)085<0842:MDDDNR>2.0.CO;2Search in Google Scholar
Ronquist, F. and J.P. Huelsenbeck. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.10.1093/bioinformatics/btg180Search in Google Scholar
Rozas, J., I. Sanchez-De, J.C. Barrio, X. Messeguer and R. Rozas. 2003. DNASP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19: 2496–2497.Search in Google Scholar
Simmons, N.B. 2005. Order Chiroptera. In: (D.E. Wilson and D.M. Reeder, eds.) Mammal species of the world: a taxonomic and geographic reference. Third edition. The Johns Hopkins University Press, Baltimore, MD. pp. 312–529.Search in Google Scholar
Šrámek, J., V. Gvoždík and P. Benda. 2013. Hidden diversity in bent-winged bats (Chiroptera: Miniopteridae) of the Western Palaearctic and adjacent regions: implications for taxonomy. Zool. Linn. Soc. 167: 165–190.10.1111/j.1096-3642.2012.00870.xSearch in Google Scholar
Tamura K., D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731–2739.Search in Google Scholar
Tate, G.H.H. 1941. Results of the Archbold Expeditions. No. 40. Notes on the vespertilionidae bats of the subfamilies Miniopterinae, Murininae, Kerivoulinae, and Nyctophilinae. Bull. Am. Mus. Nat. Hist. 78: 567–597.Search in Google Scholar
Thomas, O. 1907. On mammals from Northern Persia, presented to the National Museum by Col. A.C. Baiward. Ann. Mag. Nat. Hist. 20: 197–202.Search in Google Scholar
Thompson, J.D., D.G. Higgins and T.J. Gibson. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucl. Acids Res. 22: 4673–4680.Search in Google Scholar
Tian, L., B. Liang, K. Maeda, W. Metzner and S. Zhang. 2004. Molecular studies on the classification of Miniopterus schreibersii (Chiroptera: Vespertilionidae) inferred from mitochondrial cytochrome b sequences. Folia Zool. 53: 303–311.Search in Google Scholar
Van den Bussche, R.A. and R.J. Baker. 1993. Molecular phylogenetics of the New World bat genus Phyllostomus based on cytochrome-b DNA sequence variation. J. Mammal. 74: 793–802.10.2307/1382304Search in Google Scholar
Worthington Wilmer, J. and E. Barratt. 1996. A non-lethal method of tissue sampling for genetic studies of chiropterans. Bat Res. News 37: 1–3.Search in Google Scholar
©2015 by De Gruyter
Articles in the same Issue
- Frontmatter
- Original Studies
- Ecological drivers of group size in female Alpine chamois, Rupicapra rupicapra
- Dynamics of species composition of small non-volant mammals from the northern Cordillera Central of Colombia
- Diet of serval (Leptailurus serval) on farmlands in the Drakensberg Midlands, South Africa
- Geographical distribution, roost selection, and conservation state of cave-dwelling bats in China
- Systematic position and taxonomy of Pipistrellus deserti (Chiroptera: Vespertilionidae)
- Distribution and taxonomy of the common big-eared bat Micronycteris microtis (Chiroptera: Phyllostomidae) in South America
- Taxonomic evaluation of the bent-winged bat (Miniopterus) populations occurring in Iran inferred from mitochondrial cytochrome-b sequences
- First record of Histiotus laephotis (Thomas, 1916) from Chile and new distributional information for Histiotus montanus (Phillipi and Landbeck, 1861) (Chiroptera, Vespertilionidae)
- The subgenus Micoureus (Didelphidae: Marmosa) in Paraguay: morphometrics, distributions, and habitat associations
- PCR-RFLP identification of the endangered Pyrenean desman, Galemys pyrenaicus (Soricomorpha, Talpidae), based on faecal DNA
- First record of Calomys cerqueirai (Rodentia: Phyllotini) in Espírito Santo (Brazil) with description of the 2n=36, FNA=66 karyotype
- Short Notes
- A live trap and trapping technique for subterranean zokors (Rodentia)
- The effects of deforestation on white-lipped peccary (Tayassu pecari) home range in the southern Pantanal
- Annual Reviewer Acknowledgement
- Reviewer acknowledgement Mammalia volume 79 (2015)
Articles in the same Issue
- Frontmatter
- Original Studies
- Ecological drivers of group size in female Alpine chamois, Rupicapra rupicapra
- Dynamics of species composition of small non-volant mammals from the northern Cordillera Central of Colombia
- Diet of serval (Leptailurus serval) on farmlands in the Drakensberg Midlands, South Africa
- Geographical distribution, roost selection, and conservation state of cave-dwelling bats in China
- Systematic position and taxonomy of Pipistrellus deserti (Chiroptera: Vespertilionidae)
- Distribution and taxonomy of the common big-eared bat Micronycteris microtis (Chiroptera: Phyllostomidae) in South America
- Taxonomic evaluation of the bent-winged bat (Miniopterus) populations occurring in Iran inferred from mitochondrial cytochrome-b sequences
- First record of Histiotus laephotis (Thomas, 1916) from Chile and new distributional information for Histiotus montanus (Phillipi and Landbeck, 1861) (Chiroptera, Vespertilionidae)
- The subgenus Micoureus (Didelphidae: Marmosa) in Paraguay: morphometrics, distributions, and habitat associations
- PCR-RFLP identification of the endangered Pyrenean desman, Galemys pyrenaicus (Soricomorpha, Talpidae), based on faecal DNA
- First record of Calomys cerqueirai (Rodentia: Phyllotini) in Espírito Santo (Brazil) with description of the 2n=36, FNA=66 karyotype
- Short Notes
- A live trap and trapping technique for subterranean zokors (Rodentia)
- The effects of deforestation on white-lipped peccary (Tayassu pecari) home range in the southern Pantanal
- Annual Reviewer Acknowledgement
- Reviewer acknowledgement Mammalia volume 79 (2015)