Histological alterations in the ultimobranchial gland of teleost Heteropneustes fossilis in response to chlorpyrifos treatment
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Ajai Kumar Srivastav
,
Sanjay Kumar Srivastava
Abstract
In this study, an experiment was performed on Heteropneustes fossilis for short-term (1.76 mg/L chlorpyrifos, i.e., 0.8 of 96-h LC50) and long-term (0.44 mg/L chlorpyrifos, i.e., 0.2 of 96-h LC50) exposure. The fish were sacrificed after 24, 48, 72 and 96 h in the short-term experiment and after 7, 14, 21 and 28 days in the long-term experiment. On these intervals, blood was collected and analysis of serum calcium was done. Ultimobranchial glands were also fixed for histological study. The serum calcium levels of H. fossilis exhibit a decline after 24 h following exposure to chlorpyrifos. This decrease continues until the end of the experiment (96 h). The serum calcium levels of chronically exposed fish exhibit a decrease on day 7. Thereafter, the levels continue to fall progressively until the end of the experiment (28 days). The ultimobranchial gland of chlorpyrifos treated fish exhibits no histological change up to 48 h. After 72 h, there is a decrease in the staining response of cytoplasm of the ultimobranchial cells. The nuclear volume of these cells is slightly decreased. After 96 h following chlorpyrifos exposure, these changes become exaggerated. In chlorpyrifos-treated fish there is no change in the histological structure of the ultimobranchial gland up to 14 days. After 21 days, the cytoplasm of ultimobranchial cells stain feebly and the nuclear volume of these cells exhibits a decrease. Following 28 days treatment, the nuclear volume of these cells records a further decrease and the gland depicts vacuolization and degeneration at certain areas.
©2011 by Walter de Gruyter Berlin Boston
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Articles in the same Issue
- Publisher’s and Editor’s Note
- A new start for the Journal of Basic and Clinical Physiology and Pharmacology
- Review
- Hexavalent chromium and its effect on health: possible protective role of garlic (Allium sativum Linn)
- Original Articles
- Antioxidative and hypolipidemic efficacy of alcoholic seed extract of Swietenia macrophylla in streptozotocin diabetic rats
- Histological alterations in the ultimobranchial gland of teleost Heteropneustes fossilis in response to chlorpyrifos treatment
- Treatment with tetrahydrocannabinol (THC) prevents infertility in male cystic fibrosis mice
- Behavioral alterations in cystic fibrosis mice are prevented by cannabinoid treatment in infancy
- Anti-inflammatory and analgesic properties of the ethanolic extract of Cnidoscolus aconitifolius in rats and mice
- Birth month and longevity – monthly birth distribution in acute coronary events provoked by atherothrombosis in patients treated with percutaneous coronary intervention (PCI)
- Effect of graded doses of Caesalpinia bonducella seed extract on ovary and uterus in albino rats
