Home Quercetin promotes behavioral recovery and biomolecular changes of melanocortin-4 receptor in mice with ischemic stroke
Article
Licensed
Unlicensed Requires Authentication

Quercetin promotes behavioral recovery and biomolecular changes of melanocortin-4 receptor in mice with ischemic stroke

  • Tuhfatul Ulya , Chrismawan Ardianto EMAIL logo , Putri Anggreini , Aniek Setiya Budiatin , Dwi Setyawan and Junaidi Khotib
Published/Copyright: June 25, 2021
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 32 Issue 4

Abstract

Objectives

Ischemic stroke is known as a common causes of disability, lower psychological well-being as well as preventable death. The pathogenesis of ischemic stroke process becomes worse immediately after oxidative stress occurs. One of the flavonoids with antioxidant abilities is quercetin. This study was aimed to investigate quercetin administration on the behavioral functions (motor and sensory) and expression of melanocortin-4 receptor (MC4R) in mice with ischemic stroke.

Methods

Male ICR mice were divided into sham, stroke, stroke with quercetin 100, 150, and 200 mg/kg. The stroke model was performed by blocking the left common carotid artery for 2 h. Quercetin was intraperitoneally administered daily for seven days. Evaluation was conducted during two weeks after induction using ladder rung walking test and narrow beam test for motoric function and adhesive removal tape test for sensory function. On day-14 mice were sacrificed, MC4R expression in the dorsal striatum was determined using RT-PCR.

Results

Stroke decreased the motor, sensory function and MC4R mRNA expression in dorsal striatum. Quercetin improved motor and sensory function, and upregulated expression of MC4R.

Conclusions

Quercetin administration after ischemic stroke improves behavioral function, possibly through the upregulation of MC4R in the brain.

Keywords: dorsal striatum; ischemic stroke; melanocortin-4 receptor; motor; preventable death; quercetin
Corresponding author: Chrismawan Ardianto, Faculty of Pharmacy, Department of Clinical Pharmacy, Airlangga University, Surabaya, Indonesia, Phone: +62 822 3320 9135, E-mail:

Acknowledgments

We are grateful to Dr. Shuji Kaneko and Dr. Hisashi Shirakawa from Department of Molecular Pharmacology, Kyoto University Japan for the valuable discussion and advice. We thank to PDUPT Grant from the Indonesian Ministry of Research and Technology (Direktorat Riset dan Pengabdian Masyarakat, Deputi Bidang Penguatan Riset dan Pengembangan Kementerian Riset dan Teknologi/ Badan Riset dan Inovasi Nasional) and Tahir Foundation Professorship Program.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: All experiments were performed at the Animal Research Laboratory of the Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia in accordance with the Guide for care and use of laboratory animal issued by National Institution of Health revised in 1985. The experimental protocol was approved with letter No: 2.KE.111.07.2019 by the Animal Care and Use Committee (ACUC), Faculty of Veterinary, Universitas Airlangga.

References

1. Pei, B, Yang, M, Qi, X, Shen, X, Chen, X, Zhang, F. Quercetin ameliorates ischemia/reperfusion-induced cognitive deficits by inhibiting ASK1/JNK3/caspase-3 by enhancing the Akt signaling pathway. Biochem Biophys Res Commun 2016;478:199–205. https://doi.org/10.1016/j.bbrc.2016.07.068.Search in Google Scholar PubMed

2. Ahmad, A, Khan, MM, Hoda, MN, Raza, SS, Khan, MB, Javed, H, et al.. Quercetin protects against oxidative stress associated damages in a rat model of transient focal cerebral ischemia and reperfusion. Neurochem Res 2011;36:1360–71. https://doi.org/10.1007/s11064-011-0458-6.Search in Google Scholar PubMed

3. Ahmad, N, Ahmad, R, Naqvi, AA, Alam, MA, Ashafaq, M, Abdur Rub, R, et al.. Intranasal delivery of quercetin-loaded mucoadhesive nanoemulsion for treatment of cerebral ischaemia. Artif Cells Nanomed Biotechnol 2018;46:717–29. https://doi.org/10.1080/21691401.2017.1337024.Search in Google Scholar PubMed

4. Halliwell, B. Role of free radicals in the neurodegenerative disease: therapeutic implications for antioxidant treatment. Drugs Aging 2001;18:685–716. https://doi.org/10.2165/00002512-200118090-00004.Search in Google Scholar PubMed

5. Saleem, S, Ahmad, M, Ahmad, AS, Yousuf, S, Ansari, MA, Khan, MB, et al.. Effect of saffron (Crocus sativus) on neurobehavioral and neurochemical changes in cerebral ischemia in rats. J Med Food 2006;9:246–53. https://doi.org/10.1089/jmf.2006.9.246.Search in Google Scholar PubMed

6. Yousuf, S, Atif, F, Ahmad, M, Hoda, N, Ishrat, T, Khan, B, et al.. Resveratrol exerts its neuroprotective effect by modulating mitochondrial dysfunctions and associated cell death during cerebral ischemia. Brain Res 2009;23:242–53. https://doi.org/10.1016/j.brainres.2008.10.068.Search in Google Scholar PubMed

7. Anggreini, P, Ardianto, C, Rahmadi, M, Khotib, J. Quercetin attenuates acute predator stress exposure-evoked innate fear and behavioral perturbation. J Basic Clin Physiol Pharmacol 2019;30. https://doi.org/10.1515/jbcpp-2019-0242.Search in Google Scholar PubMed

8. Panche, AN, Diwan, AD, Chandra, SR. Flavonoids: an overview. J Nutr Sci 2016;29:e47.10.1017/jns.2016.41Search in Google Scholar PubMed PubMed Central

9. Dajas, F, Abin-Carriquiry, JA, Arredondo, F, Blasina, F, Echeverry, C, Martinez, M, et al.. Quercetin in brain disease: potential and limits. Neurochem Int 2015;89:140–8. https://doi.org/10.1016/j.neuint.2015.07.002.Search in Google Scholar PubMed

10. Setyawan, D, Setiawardani, F, Zainul, A, Sari, R. PEG 8000 increases solubility and dissolution rate of quercetin in solid dispersion system. Marmara Pharm J 2018;22:259–66. https://doi.org/10.12991/mpj.2018.63.Search in Google Scholar

11. Spaccapelo, L, Bitto, A, Galantucci, M, Ottani, A, Irrera, N, Minutoli, L, et al.. Melanocortin MC4 receptor agonists counteract late inflammatory and apoptotic responses and improve neuronal functionality after cerebral ischemia. Eur J Phamacol 2011;670:479–86. https://doi.org/10.1016/j.ejphar.2011.09.015.Search in Google Scholar

12. Snijders, AH, Takakusaki, K, Debu, B, Lozano, AM, Krishna, V, Fasano, A, et al.. Physiology of freezing of gait. Ann Neurol 2016;80:644–59. https://doi.org/10.1002/ana.24778.Search in Google Scholar

13. Lee, KB, Kim, JS, Hong, BY, Sul, B, Song, S, Sung, WJ, et al.. Brain lesions affecting gait recovery in stroke patients. Brain Behav 2017;7:e00868. https://doi.org/10.1002/brb3.868.Search in Google Scholar

14. Khotib, J, Mentari, IA, Rahmadi, M, Suharjono. Erythropoietin potential as an antiapoptotic agent in ischemic stroke using unilateral right common carotid artery occlusion (RUCCAO) model. Indian J Public Health Res Dev 2019;10:1184–9. https://doi.org/10.5958/0976-5506.2019.00871.4.Search in Google Scholar

15. Metz, GA, Whishaw, IQ. Cortical and subcortical lesions impair skilled walking in the ladder rung walking test: a new task to evaluate fore- and hindlimb stepping, placing, and co-ordination. J Neurosci Methods 2002;115:169–79. https://doi.org/10.1016/s0165-0270(02)00012-2.Search in Google Scholar

16. Allbutt, HN, Henderson, JM. Use of the narrow beam test in the rat, 6-hydroxydopamine model of Parkinson’s disease. J Neurosci Methods 2007;159:195–202. https://doi.org/10.1016/j.jneumeth.2006.07.006.Search in Google Scholar PubMed

17. Freret, T, Chazalviel, L, Roussel, S, Bernaudin, M, Schumann-Bard, P, Boulouard, M. Long-term functional outcome following transient middle cerebral artery occlusion in the rat: correlation between brain damage and behavioral impairment. Behav Neurosci 2006;120:1285–98. https://doi.org/10.1037/0735-7044.120.6.1285.Search in Google Scholar PubMed

18. Ardianto, C, Yonemochi, N, Yamamoto, S, Yang, L, Takenoya, F, Shioda, S, et al.. Opioid systems in the lateral hypothalamus regulate feeding behavior through orexin and GABA neurons. Neuroscience 2016;320:183–93. https://doi.org/10.1016/j.neuroscience.2016.02.002.Search in Google Scholar PubMed

19. Barrenetxe, J, Aranguren, P, Grijalba, A, Martinez-Penuela, JM, Marzo, F, Urdaneta, E. Effect of dietary quercetin and sphingomyelin on ıntestinal nutrient absorption and animal growth. Br J Nutr 2006;95:455–61. https://doi.org/10.1079/bjn20051651.Search in Google Scholar PubMed

20. Yamamoto, Y, Oue, E. Antihypertensive effect of quercetin in rats fed with a high-fat high-sucrose diet. Biosci Biotechnol Biochem 2006;70:933–9. https://doi.org/10.1271/bbb.70.933.Search in Google Scholar PubMed

21. Du, G, Zhao, Z, Chen, Y, Li, Z, Tian, Y, Liu, Z, et al.. Quercetin protects rat cortical neurons against traumatic brain ınjury. Mol Med Rep 2018;17:7859–65. https://doi.org/10.3892/mmr.2018.8801.Search in Google Scholar

22. Chen, M, Qin, J, Chen, S, Yao, L, Zhang, L, Yin, Z, et al.. Quercetin promotes motor and sensory function recovery following sciatic nerve-crush ınjury in C57BL/6J mice. J Nutr Biochem 2017;46:57–67. https://doi.org/10.1016/j.jnutbio.2017.04.006.Search in Google Scholar

23. Carniglia, L, Ramírez, D, Durand, D, Saba, J, Turati, J, Caruso, C, et al.. Neuropeptides and microglial activation in inflammation, pain, and neurodegenerative diseases. Mediat Inflamm 2017;2017:1–23. https://doi.org/10.1155/2017/5048616.Search in Google Scholar

24. Pandit, R, Van Der Zwaal, EM, Luijendijk, MCM, Brans, MAD, Van Rozen, AJ, Ophuis, RJAO, et al.. Central melanocortins regulate the motivation for sucrose reward. PLoS One 2015;10:1–15. https://doi.org/10.1371/journal.pone.0121768.Search in Google Scholar

25. Mountjoy, KG, Guan, J, Elia, CJ, Sirimanne, ES, Williams, CE. Melanocortin-4 receptor messenger RNA expression is up-regulated in the non-damaged striatum following unilateral hypoxic-ischaemic brain injury. Neuroscience 1999;89:183–90. https://doi.org/10.1016/s0306-4522(98)00285-1.Search in Google Scholar

26. Quintanilla, RA, Pérez, MJ, Aranguiz, A, Tapia-Monsalves, C, Mendez, G. Activation of the melanocortin-4 receptor prevents oxidative damage and mitochondrial dysfunction in cultured hippocampal neurons exposed to ethanol. Neurotox Res 2020.10.1007/s12640-020-00204-1Search in Google Scholar PubMed

27. Costa, LG, Garrick, JM, Roque, PJ, Pellacani, C. Mechanisms of neuroprotection by quercetin: counteracting oxidative stress and more. Oxid Med Cell Longev 2016;2016:2986796. https://doi.org/10.1155/2016/2986796.Search in Google Scholar PubMed PubMed Central

28. Alrawaiq, NS, Abdullah, A. A review of flavonoid quercetin: metabolism, bioactivity and antioxidant properties. Int J PharmTech Res 2014;6:933–41.Search in Google Scholar
Received: 2020-11-30
Accepted: 2021-03-03
Published Online: 2021-06-25

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Cost of illness of diabetes mellitus in Indonesia: a systematic review
  4. Social media health interventions to improve diabetes mellitus patient outcome: a systematic review
  5. Developing pharmacokinetics–pharmacodynamics model of valproic acid syrup based on prediction of population pharmacokinetics parameter and seizure frequency in Indonesian pediatric epilepsy outpatients
  6. Acetylcholinesterase inhibitory activity of extract and fractions from the root of Rauvolfia serpentina(L.) Bth.ex Kurz
  7. Green tea and its active compound epigallocathechin-3-gallate (EGCG) inhibit neuronal apoptosis in a middle cerebral artery occlusion (MCAO) model
  8. The effects of quercetin on nicotine-induced reward effects in mice
  9. Resveratrol ameliorates physical and psychological stress-induced depressive-like behavior
  10. Translation and cross-cultural adaption of an instrument measuring patient’s well-being under treatment for schizophrenia
  11. Quercetin promotes behavioral recovery and biomolecular changes of melanocortin-4 receptor in mice with ischemic stroke
  12. Knowledge and attitudes of healthcare professionals on prescribing errors
  13. Inhibition of Ras and STAT3 activity of 4-(tert-butyl)-N-carbamoylbenzamide as antiproliferative agent in HER2-expressing breast cancer cells
  14. Predicting the molecular mechanism of glucosamine in accelerating bone defect repair by stimulating osteogenic proteins
  15. Larvicidal toxicity and parasporal inclusion of native Bacillus thuringiensis BK5.2 against Aedes aegypti
  16. Synthesis, ADMET predictions, molecular docking studies, and in-vitro anticancer activity of some benzoxazines against A549 human lung cancer cells
  17. Thymoquinone and its derivatives against breast cancer with HER2 positive: in silico studies of ADMET, docking and QSPR
  18. Assessment of patient understanding of their conventional cardiac medicines and herbal prepared/derived products: preliminary survey and interviews with selected community-dwelling elderly patients in the Philippines
  19. The development and validation of the health belief model questionnaire for measuring factors affecting adherence in the elderly with hypertension
  20. Analysis of the side effect of QTc interval prolongation in the bedaquiline regimen in drug resistant tuberculosis patients
  21. Shallot skin profilling, computational evaluation of physicochemical properties, ADMET, and molecular docking of its components against P2Y12 receptor
  22. Analysis of HMGB-1 level before and after providing atorvastatin standard therapy in coronary artery disease patients with type-2 diabetes mellitus compared to without type-2 diabetes mellitus
  23. Analysis of matrix metalloproteinase-9 levels among acute heart failure patients with ACE inhibitor therapy (Dr. Soetomo Regional General Hospital, Surabaya)
  24. The correlation between self-related adherence, asthma-related quality of life and control of asthma in adult patients
  25. Providing counseling through home pharmacy care (HPC) for hemodialysis patients with hypertension in lowering blood pressure
  26. Community knowledge and attitude in recognizing asthma symptoms and using medication for asthma attacks: a cross-sectional study
  27. A study of anticoagulant therapy in patients with coronary artery disease
  28. The association of FKBP5 polymorphism with asthma susceptibility in asthmatic patients
  29. Gastroprotective effect of fluvoxamine and ondansetron on stress-induced gastric ulcers in mice
  30. Osteoblast iron genes: real time PCR and microarray hybridization approach under hyperoxia
  31. Attenuation of hyperplasia in lung parenchymal and colonic epithelial cells in DMBA-induced cancer by administering Andrographis paniculata Nees extract using animal model
  32. N-nitrosodiethylamine induces inflammation of liver in mice
  33. AST/ALT levels, MDA, and liver histopathology of Echinometra mathaei ethanol extract on paracetamol-induced hepatotoxicity in rats
  34. Development, characterization, molecular docking, and in vivo skin penetration of coenzyme Q10 nanostructured lipid carriers using tristearin and stearyl alcohol for dermal delivery
  35. The effect of Camellia sinensis (green tea) with its active compound EGCG on neuronal cell necroptosis in Rattus norvegicus middle cerebral artery occlusion (MCAO) model
  36. Hepatoprotective effect of ethanolic extract of sugarcane (Saccharum officinarum Linn.) leaves
  37. Correlation between the exposure time to mobile devices and the prevalence of evaporative dry eyes as one of the symptoms of computer vision syndrome among Senior High School students in East Java, Indonesia
  38. The effect of various high-fat diet on liver histology in the development of NAFLD models in mice
  39. Fabrication and characterization of bovine hydroxyapatite-gelatin-alendronate scaffold cross-linked by glutaraldehyde for bone regeneration
  40. Health related quality of life among postmenopausal woman with hormone responsive HER2− breast cancer in Indonesia
  41. Gender differences in the blood glucose type 2 diabetes patients with combination rapid and long acting insulin therapy
  42. Correlation of dietary iron intake and serum iron with thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels in adult hyperthyroid patients
  43. The effect of pillbox use and education by pharmacist toward medication adherence in diabetes mellitus patients in a Primary Health Care Center in Mataram
  44. Variation concentration effect of propyleneglycol, glycerin, and polyethyleneglycol 400 to physical properties and dissolution rate of loratadine liquisolid tablet
  45. Role of Centella asiatica and ceramide in skin barrier improvement: a double blind clinical trial of Indonesian batik workers
  46. Secondary metabolite and antipyretic effects of Maja (Crescentia cujete L.) in fever-induced mice
  47. Hydration effect on kidney function and serum electrolyte in children with tumor lysis syndrome (TLS) and risk of TLS
  48. Drug utilization study and cost analysis of adult β-thalassemia major patient therapy at Dr. Soetomo General Hospital Surabaya
  49. The role of hyperbaric oxygen to platelet aggregation in noninsulin-dependent diabetes mellitus (NIDDM)
  50. Cocrystal formation of loratadine-succinic acid and its improved solubility
  51. The role of chondroitin sulfate to bone healing indicators and compressive strength
  52. The effects of quercetin on the expression of SREBP-1c mRNA in high-fat diet-induced NAFLD in mice
  53. Analysis of stress ulcer prophylaxis drug regimentation in surgical patients
  54. The stability and irritability study of the chitosan–Aloe vera spray gel as wound healing
  55. Effectiveness of citicoline in pediatric patients with refractive amblyopia in Surabaya, East Java, Indonesia
  56. The thermodynamic study of p-methoxycinnamic acid inclusion complex formation, using β-cyclodextrin and hydroxypropyl-β-cyclodextrin
  57. The effect of chitosan type and drug-chitosan ratio on physical characteristics and release profile of ketoprofen microparticles prepared by spray drying
  58. The maximum dose and duration in the therapy single use methotrexate to achieve remission by rheumatoid arthritis patients through disease activity score 28 (DAS28)
  59. Knowledge, attitudes, and practices (KAP) towards COVID-19 among university students in Pakistan: a cross-sectional study
  60. The impact of glutaraldehyde on the characteristics of bovine hydroxyapatite-gelatin based bone scaffold as gentamicin delivery system
  61. Analysis of the use of antibiotics profile and factors of surgical site infections study on digestive and oncology surgeries
  62. Second internal transcribed spacer (ITS-2) as genetic marker for molecular characterization of Sarcoptes scabiei in rabbits from several areas of East Java, Indonesia
  63. Design of gossypetin derivatives based on naturally occurring flavonoid in Hibiscus sabdariffa and the molecular docking as antibacterial agents
  64. Discovery of new targeting agents against GAPDH receptor for antituberculosis drug delivery
  65. The effect of red passion fruit (Passiflora edulis Sims.) fermentation time on its activity against Extended Strain Methicillin-Resistant (ESBL) Escherichia coli and Methicillin-Resistant Staphylococcus aureus (MRSA)
  66. Antibiotic use on acute respiratory tract infection nonpneumonia and nonspecific diarrhea in Primary Health Care Centre in Banjarbaru City, South Kalimantan, Indonesia
  67. Screening of anti-HIV activities in ethanol extract and fractions from Ficus fistulosa leaves
  68. The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics
  69. Profile of gyrA gene mutation in clinical isolate of levofloxacin resistant Escherichia coli
  70. Antimicrobial activity of Centella asiatica and Gigantochloa apus
  71. Drug-related problems of antibiotic use in gastroenteritis related to patient therapy outcomes at Universitas Gadjah Mada Hospital
  72. The impact of suitability of empirical antibiotics use on therapeutic outcome of respiratory tract infection patients at inpatient wards of Universitas Gadjah Mada Academic Hospital
  73. Genetic profile mutation rpoB in clinical isolate of rifampicin-resistant Staphylococcus aureus
  74. Hematological side effect analysis of linezolid in MDR-TB patients with individual therapy
  75. Adverse drug reaction and its management in tuberculosis patients with multidrug resistance: a retrospective study
  76. Analysis of prophylactic antibiotic use and risk factor of postoperative infection in urological surgery patients
  77. Molecular docking studies of Nigella sativa L and Curcuma xanthorrhiza Roxb secondary metabolites against histamine N-methyltransferase with their ADMET prediction
  78. Prediction of compounds with antiosteoporosis activity in Chrysophyllum cainito L. leaves through in silico approach
  79. Phyllanthin and hypophyllanthin, the isolated compounds of Phyllanthus niruri inhibit protein receptor of corona virus (COVID-19) through in silico approach
  80. Cratoxylum sumatranum stem bark exhibited antimalarial activity by Lactate Dehydrogenase (LDH) assay
  81. Endophytic fungi inhabiting Physalis angulata L. plant: diversity, antioxidant, and antibacterial activities of their ethyl acetate extracts
  82. Exploration of several plants from Baung Forest on bone formation cell models
  83. In vitro antimalarial activity of Garcinia parvifolia Miq. Stem extracts and fractions on Plasmodium falciparum lactate dehydrogenase (LDH) assay
  84. Antioxidant and antiviral potency of Begonia medicinalis fractions
  85. Artocarpus sericicarpus stem bark contains antimalarial substances against Plasmodium falciparum
  86. Formulation and characterization of Eleutherine palmifolia extract-loaded self-nanoemulsifying drug delivery system (SNEDDS)
  87. Analytical method for the determination of curcumin entrapped in polymeric micellar powder using HPLC
  88. Challenges in the provision of natural medicines by community pharmacists in East Java Province, Indonesia
  89. In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent
  90. Inhibitory activity of Urena lobata leaf extract on alpha-amylase and alpha-glucosidase: in vitro and in silico approach
  91. Case Report
  92. Effect of hydrocortisone on hypocorticolism caused by pituitary adenoma
https://doi.org/10.1515/jbcpp-2020-0490
Keywords for this article
dorsal striatum; ischemic stroke; melanocortin-4 receptor; motor; preventable death; quercetin

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Cost of illness of diabetes mellitus in Indonesia: a systematic review
  4. Social media health interventions to improve diabetes mellitus patient outcome: a systematic review
  5. Developing pharmacokinetics–pharmacodynamics model of valproic acid syrup based on prediction of population pharmacokinetics parameter and seizure frequency in Indonesian pediatric epilepsy outpatients
  6. Acetylcholinesterase inhibitory activity of extract and fractions from the root of Rauvolfia serpentina(L.) Bth.ex Kurz
  7. Green tea and its active compound epigallocathechin-3-gallate (EGCG) inhibit neuronal apoptosis in a middle cerebral artery occlusion (MCAO) model
  8. The effects of quercetin on nicotine-induced reward effects in mice
  9. Resveratrol ameliorates physical and psychological stress-induced depressive-like behavior
  10. Translation and cross-cultural adaption of an instrument measuring patient’s well-being under treatment for schizophrenia
  11. Quercetin promotes behavioral recovery and biomolecular changes of melanocortin-4 receptor in mice with ischemic stroke
  12. Knowledge and attitudes of healthcare professionals on prescribing errors
  13. Inhibition of Ras and STAT3 activity of 4-(tert-butyl)-N-carbamoylbenzamide as antiproliferative agent in HER2-expressing breast cancer cells
  14. Predicting the molecular mechanism of glucosamine in accelerating bone defect repair by stimulating osteogenic proteins
  15. Larvicidal toxicity and parasporal inclusion of native Bacillus thuringiensis BK5.2 against Aedes aegypti
  16. Synthesis, ADMET predictions, molecular docking studies, and in-vitro anticancer activity of some benzoxazines against A549 human lung cancer cells
  17. Thymoquinone and its derivatives against breast cancer with HER2 positive: in silico studies of ADMET, docking and QSPR
  18. Assessment of patient understanding of their conventional cardiac medicines and herbal prepared/derived products: preliminary survey and interviews with selected community-dwelling elderly patients in the Philippines
  19. The development and validation of the health belief model questionnaire for measuring factors affecting adherence in the elderly with hypertension
  20. Analysis of the side effect of QTc interval prolongation in the bedaquiline regimen in drug resistant tuberculosis patients
  21. Shallot skin profilling, computational evaluation of physicochemical properties, ADMET, and molecular docking of its components against P2Y12 receptor
  22. Analysis of HMGB-1 level before and after providing atorvastatin standard therapy in coronary artery disease patients with type-2 diabetes mellitus compared to without type-2 diabetes mellitus
  23. Analysis of matrix metalloproteinase-9 levels among acute heart failure patients with ACE inhibitor therapy (Dr. Soetomo Regional General Hospital, Surabaya)
  24. The correlation between self-related adherence, asthma-related quality of life and control of asthma in adult patients
  25. Providing counseling through home pharmacy care (HPC) for hemodialysis patients with hypertension in lowering blood pressure
  26. Community knowledge and attitude in recognizing asthma symptoms and using medication for asthma attacks: a cross-sectional study
  27. A study of anticoagulant therapy in patients with coronary artery disease
  28. The association of FKBP5 polymorphism with asthma susceptibility in asthmatic patients
  29. Gastroprotective effect of fluvoxamine and ondansetron on stress-induced gastric ulcers in mice
  30. Osteoblast iron genes: real time PCR and microarray hybridization approach under hyperoxia
  31. Attenuation of hyperplasia in lung parenchymal and colonic epithelial cells in DMBA-induced cancer by administering Andrographis paniculata Nees extract using animal model
  32. N-nitrosodiethylamine induces inflammation of liver in mice
  33. AST/ALT levels, MDA, and liver histopathology of Echinometra mathaei ethanol extract on paracetamol-induced hepatotoxicity in rats
  34. Development, characterization, molecular docking, and in vivo skin penetration of coenzyme Q10 nanostructured lipid carriers using tristearin and stearyl alcohol for dermal delivery
  35. The effect of Camellia sinensis (green tea) with its active compound EGCG on neuronal cell necroptosis in Rattus norvegicus middle cerebral artery occlusion (MCAO) model
  36. Hepatoprotective effect of ethanolic extract of sugarcane (Saccharum officinarum Linn.) leaves
  37. Correlation between the exposure time to mobile devices and the prevalence of evaporative dry eyes as one of the symptoms of computer vision syndrome among Senior High School students in East Java, Indonesia
  38. The effect of various high-fat diet on liver histology in the development of NAFLD models in mice
  39. Fabrication and characterization of bovine hydroxyapatite-gelatin-alendronate scaffold cross-linked by glutaraldehyde for bone regeneration
  40. Health related quality of life among postmenopausal woman with hormone responsive HER2− breast cancer in Indonesia
  41. Gender differences in the blood glucose type 2 diabetes patients with combination rapid and long acting insulin therapy
  42. Correlation of dietary iron intake and serum iron with thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels in adult hyperthyroid patients
  43. The effect of pillbox use and education by pharmacist toward medication adherence in diabetes mellitus patients in a Primary Health Care Center in Mataram
  44. Variation concentration effect of propyleneglycol, glycerin, and polyethyleneglycol 400 to physical properties and dissolution rate of loratadine liquisolid tablet
  45. Role of Centella asiatica and ceramide in skin barrier improvement: a double blind clinical trial of Indonesian batik workers
  46. Secondary metabolite and antipyretic effects of Maja (Crescentia cujete L.) in fever-induced mice
  47. Hydration effect on kidney function and serum electrolyte in children with tumor lysis syndrome (TLS) and risk of TLS
  48. Drug utilization study and cost analysis of adult β-thalassemia major patient therapy at Dr. Soetomo General Hospital Surabaya
  49. The role of hyperbaric oxygen to platelet aggregation in noninsulin-dependent diabetes mellitus (NIDDM)
  50. Cocrystal formation of loratadine-succinic acid and its improved solubility
  51. The role of chondroitin sulfate to bone healing indicators and compressive strength
  52. The effects of quercetin on the expression of SREBP-1c mRNA in high-fat diet-induced NAFLD in mice
  53. Analysis of stress ulcer prophylaxis drug regimentation in surgical patients
  54. The stability and irritability study of the chitosan–Aloe vera spray gel as wound healing
  55. Effectiveness of citicoline in pediatric patients with refractive amblyopia in Surabaya, East Java, Indonesia
  56. The thermodynamic study of p-methoxycinnamic acid inclusion complex formation, using β-cyclodextrin and hydroxypropyl-β-cyclodextrin
  57. The effect of chitosan type and drug-chitosan ratio on physical characteristics and release profile of ketoprofen microparticles prepared by spray drying
  58. The maximum dose and duration in the therapy single use methotrexate to achieve remission by rheumatoid arthritis patients through disease activity score 28 (DAS28)
  59. Knowledge, attitudes, and practices (KAP) towards COVID-19 among university students in Pakistan: a cross-sectional study
  60. The impact of glutaraldehyde on the characteristics of bovine hydroxyapatite-gelatin based bone scaffold as gentamicin delivery system
  61. Analysis of the use of antibiotics profile and factors of surgical site infections study on digestive and oncology surgeries
  62. Second internal transcribed spacer (ITS-2) as genetic marker for molecular characterization of Sarcoptes scabiei in rabbits from several areas of East Java, Indonesia
  63. Design of gossypetin derivatives based on naturally occurring flavonoid in Hibiscus sabdariffa and the molecular docking as antibacterial agents
  64. Discovery of new targeting agents against GAPDH receptor for antituberculosis drug delivery
  65. The effect of red passion fruit (Passiflora edulis Sims.) fermentation time on its activity against Extended Strain Methicillin-Resistant (ESBL) Escherichia coli and Methicillin-Resistant Staphylococcus aureus (MRSA)
  66. Antibiotic use on acute respiratory tract infection nonpneumonia and nonspecific diarrhea in Primary Health Care Centre in Banjarbaru City, South Kalimantan, Indonesia
  67. Screening of anti-HIV activities in ethanol extract and fractions from Ficus fistulosa leaves
  68. The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics
  69. Profile of gyrA gene mutation in clinical isolate of levofloxacin resistant Escherichia coli
  70. Antimicrobial activity of Centella asiatica and Gigantochloa apus
  71. Drug-related problems of antibiotic use in gastroenteritis related to patient therapy outcomes at Universitas Gadjah Mada Hospital
  72. The impact of suitability of empirical antibiotics use on therapeutic outcome of respiratory tract infection patients at inpatient wards of Universitas Gadjah Mada Academic Hospital
  73. Genetic profile mutation rpoB in clinical isolate of rifampicin-resistant Staphylococcus aureus
  74. Hematological side effect analysis of linezolid in MDR-TB patients with individual therapy
  75. Adverse drug reaction and its management in tuberculosis patients with multidrug resistance: a retrospective study
  76. Analysis of prophylactic antibiotic use and risk factor of postoperative infection in urological surgery patients
  77. Molecular docking studies of Nigella sativa L and Curcuma xanthorrhiza Roxb secondary metabolites against histamine N-methyltransferase with their ADMET prediction
  78. Prediction of compounds with antiosteoporosis activity in Chrysophyllum cainito L. leaves through in silico approach
  79. Phyllanthin and hypophyllanthin, the isolated compounds of Phyllanthus niruri inhibit protein receptor of corona virus (COVID-19) through in silico approach
  80. Cratoxylum sumatranum stem bark exhibited antimalarial activity by Lactate Dehydrogenase (LDH) assay
  81. Endophytic fungi inhabiting Physalis angulata L. plant: diversity, antioxidant, and antibacterial activities of their ethyl acetate extracts
  82. Exploration of several plants from Baung Forest on bone formation cell models
  83. In vitro antimalarial activity of Garcinia parvifolia Miq. Stem extracts and fractions on Plasmodium falciparum lactate dehydrogenase (LDH) assay
  84. Antioxidant and antiviral potency of Begonia medicinalis fractions
  85. Artocarpus sericicarpus stem bark contains antimalarial substances against Plasmodium falciparum
  86. Formulation and characterization of Eleutherine palmifolia extract-loaded self-nanoemulsifying drug delivery system (SNEDDS)
  87. Analytical method for the determination of curcumin entrapped in polymeric micellar powder using HPLC
  88. Challenges in the provision of natural medicines by community pharmacists in East Java Province, Indonesia
  89. In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent
  90. Inhibitory activity of Urena lobata leaf extract on alpha-amylase and alpha-glucosidase: in vitro and in silico approach
  91. Case Report
  92. Effect of hydrocortisone on hypocorticolism caused by pituitary adenoma
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 19.10.2025 from https://www.degruyterbrill.com/document/doi/10.1515/jbcpp-2020-0490/html
Scroll to top button