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Modulatory properties of cardiac and quercetin glycosides from Dacryodes edulis seeds during L-NAME-induced vascular perturbation

  • Peter Uchenna Amadi EMAIL logo , Emmanuel Nnabugwu Agomuo and Chiamaka Winifred Adumekwe
Published/Copyright: July 11, 2020
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 31 Issue 5

Abstract

Background

Numerous food wastes have been identified to possess potent bioactive compounds used for the treatment of several diseases. Therefore this study evaluated the potentials of cardiac and quercetin glycosides extracted from Dacryodes edulis seeds to reverse vascular and endothelial damage (VAED).

Methods

The glycoside composition of the seeds was extracted using standard methods and characterized by gas chromatography. We then recruited rats with L-NAME-induced VAED based on confirmatory biomarkers cardiac troponin (CnT), cellular adhesion molecule (VCAM-1), lipoprotein associated phospholipase A2 (Lp-PLA2), RAAS, VWF, endothelin, eNOx, and homocysteine. Only rats that showed total alterations of all biomarkers were recruited into the respective experimental groups and treated with either metaprolol succinate (met.su) + losartan or glycoside extracts of D. edulis seeds (NPSG).

Results

Chromatographic isolation of glycosides in the seed showed predominance of artemetin (1.59 mg/100 g), amygdalin (3.68 mg/100 g), digitoxin (19.21 mg/100 g), digoxin (27.23 mg/100 g), avicularin (133.59 mg/100 g), and hyperoside (481.76 mg/100 g). We observed decreased water intake and higher heart beats under vascular damage as the experiment progressed up to the fourth week. The met.su + losartan and H.D NPSG proved effective in restoring troponin, but both doses of NPSG normalized the VCAM-1 and RAAS activities excluding aldosterone and Lp-PLA2. Among the endothelial dysfunction biomarkers, H.D NPSG produced equivalent effects to met.su + losartan towards restoring the eNOx and VWF activities, but showed higher potency in normalizing the endothelin and Hcy levels.

Conclusions

We thus propose that the synergistic effect of the isolated glycosides from D. edulis shown in our study proved potent enough at high doses in treatment of vascular and endothelial dysfunction.

Keywords: biomarker; Dacryodes edulis; endothelial damage; glycosides; vascular dysfunction
Corresponding author: Peter Uchenna Amadi, Department of Biochemistry, University of Port Harcourt, Choba, Rivers State, Nigeria; and Department of Biochemistry, Imo State Universit, Owerri, Imo State, Nigeria, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: The local Institutional Review Board, Biochemistry, Imo State University Ethics Committee appropriated the protocol and issued an approval number; IMSU/BCM/ETS/20180716. All experimental protocol aligned with stipulations of World Medical Association Declaration of Helsinki regarding ethical conduct of research involving animals.

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Supplementary Material

Supplementary material to this article can be found online at https://doi.org/10.1515/jbcpp-2019-0116.

Received: 2019-05-02
Accepted: 2020-03-06
Published Online: 2020-07-11

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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  9. Modulatory properties of cardiac and quercetin glycosides from Dacryodes edulis seeds during L-NAME-induced vascular perturbation
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https://doi.org/10.1515/jbcpp-2019-0116
Keywords for this article
biomarker; Dacryodes edulis; endothelial damage; glycosides; vascular dysfunction

Articles in the same Issue

  1. Review
  2. Phytochemical, ethanomedicinal and pharmacological applications of escin from Aesculus hippocastanum L. towards future medicine
  3. Original Articles
  4. Relationship between trough level of tyrosine kinase inhibitor (imatinib and nilotinib) and BCR-ABL ratios in an Indonesian chronic-phase chronic myeloid leukemia (CML) population
  5. Dietary supplementation of Pleurotus tuber regium in rat feed ameliorates metabolic and hematotoxicity induced by carbon tetrachloride
  6. Protective effects and chemical composition of Corchorus olitorius leaf fractions against isoproterenol-induced myocardial injury through p65NFkB-dependent anti-apoptotic pathway in rats
  7. Natural limonoids protect mice from alcohol-induced liver injury
  8. Production of the secondary metabolite catechin by in vitro cultures of Camellia sinensis L
  9. Modulatory properties of cardiac and quercetin glycosides from Dacryodes edulis seeds during L-NAME-induced vascular perturbation
  10. Case Report
  11. Enoxaparin induced reactive thrombocytosis: a rare adverse drug reaction
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