Home Optimization of ultrasonic extraction of Lycium barbarum polysaccharides using response surface methodology
Article
Licensed
Unlicensed Requires Authentication

Optimization of ultrasonic extraction of Lycium barbarum polysaccharides using response surface methodology

  • Jitian Song EMAIL logo , Shi Dongqi , Su Hang , Feng Yongxia and Tian Wei
Published/Copyright: September 17, 2020

Abstract

Ultrasonic extraction was a new development method to achieve high-efficiency extraction of Lycium barbarum polysaccharides instead of hot water extraction. In this paper, the single factor method combined with the box Behnken design of response surface method was used to study the influence of extraction time, extraction temperature, material liquid ratio and ultrasonic power on the extraction rate of L. barbarum polysaccharide. The results indicated that the best extraction rate of L. barbarum polysaccharide was 12.54 ± 0.12% under the conditions of 80 min for extraction time, 73 °C for extraction temperature, 1 g:38 mL for material to liquid ratio, and 185 W for ultrasonic power. Under the same operating conditions, the yield of L. barbarum polysaccharide using ultrasonic extraction was 83.3%, which was higher than that hot water extraction. Moreover, the extraction time of ultrasonic extraction was only 47% of that using hot water extraction. This suggested that there was great potentials of using ultrasonic extraction in the realization of high-efficiency extraction of L. barbarum polysaccharide. The results of this study could also provide a theoretical basis for the coupling of ultrasonic extraction and ultrasonic concentration process to develop the integrated equipment of both ultrasonic extraction and ultrasonic concentration.


Corresponding author: Jitian Song, Tianjin Key Laboratory of Integrated Design and On-line Monitoring for Light Industry & Food Machinery and Equipment, College of Mechanical Engineering, Tianjin University of Science and Technology, 300222, Tianjin, China; and International Science and Technology Cooperation Base of Low-Carbon Green Process Equipment, 300222, Tianjin, China, Tel.:+86 022 60272125, E-mail:

Funding source: Key Project of Philosophy and Social Sciences Research, Ministry of Education (China) “Research on Green Design in Sustainable Development”

Award Identifier / Grant number: 16JZD014

Funding source: Hebei provincial and university science & technology cooperation and development fund support project

Award Identifier / Grant number: 130127

Acknowledgments

This research was supported by the Hebei provincial and university science & technology cooperation and development fund support project (No. 130127) and the Key Project of Philosophy and Social Sciences Research, Ministry of Education (China) “Research on Green Design in Sustainable Development” (contract No. 16JZDH014, approval No. 16JZD014).

  1. Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: Hebei Provincial and University Science & Technology Cooperation and Development fund support project (No. 130127) and the Key Project of Philosophy and Social Sciences Research, Ministry of Education (China) “Research on Green Design in Sustainable Development” (contract No. 16JZDH014, approval No. 16JZD014).

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

References

1. Li, XM, Ma, YL, Liu, XJ. Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice. J Ethnopharmacol 2007;111:504–11. https://doi.org/10.1016/j.jep.2006.12.024.Search in Google Scholar PubMed

2. Peng, Y, Ma, C, Li, YW, Sze-Yin Leung, K, Jiang, Z-H, Zhao, Z. Quantification of -thin dipalmitate and total carotenoids in Lycium fruits(Fructus). Plant Foods Hum Nutr 2005;60:161–4. https://doi.org/10.1007/s11130-005-9550-5.Search in Google Scholar PubMed

3. Dong, JZ, Lu, DY, Wang, Y. Analysis of flavonoids from leaves of cultivated Lycium barbarum. Plant Foods Hum Nutr 2009;64:199–204. https://doi.org/10.1007/s11130-009-0128-x.Search in Google Scholar PubMed

4. Willcox, JK, Ash, SL, Catignani, GL. Antioxidants and prevention of hronic isease. Crit Rev Food Sci Nutr 2004;44:275–95. https://doi.org/10.1080/10408690490468489.Search in Google Scholar PubMed

5. Ma, WP, Ni, ZJ, Li, CH, Min, C. Changes of the main carotenoid pigment contents during the drying processes of the different harvest stage fruits of Lycium barbarum L. Agr Sci China 2008;7: 363–9. https://doi.org/10.1016/s1671-2927(08)60077-2.Search in Google Scholar

6. Amagase, H, Sun, B, Borek, C. Lycium barbarum (GOJI) juice improves in vivo antioxidant biomarkers in serum of healthy adults. Nutr Res (NY) 2009;29:19–25. https://doi.org/10.1016/j.nutres.2008.11.005.Search in Google Scholar PubMed

7. Song, MK, Salam, NK, Roufogalis, BD, Huang, THW. Lycium barbarum (Goji Berry) extracts and its taurine component inhibit PPAR-γ-dependent gene transcription in human retinal pigment epithelial cells: possible implications for diabetic retinopathy treatment. Biochem Pharmacol 2011;82:1209–18. https://doi.org/10.1016/j.bcp.2011.07.089.Search in Google Scholar PubMed

8. Wang, CC, Chang, SC, Inbaraj, BS, Chen, BH. Isolation of carotenoids, flavonoids and polysaccharides from Lycium barbarum L and evaluation of antioxidant activity. Food Chem 2010;120:184–92. https://doi.org/10.1016/j.foodchem.2009.10.005.Search in Google Scholar

9. Abdelmalek, MF, Angulo, P, Jorgensen, RA, Sylvestre, PB, Lindor, KD. Betaine, a promising new agent for patients with nonalcoholic : results of a pilot study. Am J Gastroenterol 2001;96:2711–7. https://doi.org/10.1111/j.1572-0241.2001.04129.x.Search in Google Scholar PubMed

10. Zhang, J, Jia, S, Liu, Y, Wu, S, Ran, J. Optimization of enzyme-assisted extraction of the Lycium barbarum polysaccharides using response surface methodology. Carbohydr Polym 2011;86:1089–92. https://doi.org/10.1016/j.carbpol.2011.06.027.Search in Google Scholar

11. Liu, YL, Yin, RQ, Liang, SS, Duan, YL, Yao, JH, Duan, YL, et al. Effect of dietary Lycium barbarum polysaccharide on growth performance and immune function of broilers. J Appl Poultry Res 2017;26:200–8. https://doi.org/10.3382/japr/pfw063.Search in Google Scholar

12. Ji, X, Peng, Q, Li, H, Liu, F, Wang, M. Chemical characterization and anti-inflammatory activity of polysaccharides from Zizyphus jujube cv. Muzao, Int J Food Eng 2016,13:20160382. https://doi.org/10.1515/ijfe-2016-0382.Search in Google Scholar

13. Luo, Q, Li, JJ, Cui, XY, Yan, J, Zhao, Q, Xiang, C. The effect of Lycium barbarum polysaccharides on the male rats reproductive system and spermatogenic cell apoptosis exposed to low-dose ionizing irradiation. J Ethnopharmacol 2014;154:249–58. https://doi.org/10.1016/j.jep.2014.04.013.Search in Google Scholar PubMed

14. Zou, S, Zhang, X, Yao, W, Niu, Y, Gao, X. Structure characterization and hypoglycemic activity of a polysaccharide isolated from the fruit of Lycium barbarum. Carbohydr Polym 2010;80:1161–7. https://doi.org/10.1016/j.carbpol.2010.01.038.Search in Google Scholar

15. Guo, X, Zou, X, Sun, M. Optimization of extraction process by response surface methodology and preliminary characterization of polysaccharides from Phellinus igniarius. Carbohydr Polym 2010;80:344–9. https://doi.org/10.1016/j.carbpol.2009.11.028.Search in Google Scholar

16. Lin, Y, Zeng, HY, Wang, K, Lin, H, Li, P, Huang, Y, et al. Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity. Int J Biol Macromol 2019;136:305–15. https://doi.org/10.1016/j.ijbiomac.2019.06.064.Search in Google Scholar PubMed

17. Chai, YY, Kan, LB, Zhao, M. Enzymatic extraction optimization, anti-HBV and antioxidant activities of polysaccharides from Viscum coloratum (Kom.) Nakai. Int J Biol Macromol 2019;134:588–94. https://doi.org/10.1016/j.ijbiomac.2019.04.173.Search in Google Scholar PubMed

18. Sheng, Z, Wang, B, Zhao, J, Yu, W. Optimization of ultrasonic-assisted extraction for inocembrin from lospopuli using response surface methodology. Int J Food Eng 2017;13:392–5. https://doi.org/10.1515/ijfe-2016-0428.Search in Google Scholar

19. Li, Y, Fabiano-Tixier, AS, Tomao, V, Cravotto, G, Chemat, F. Green ultrasound-assisted extraction of carotenoids based on the bio-refinery concept using sunflower oil as an alternative solvent. Ultrason Sonochem 2013;20:12–18. https://doi.org/10.1016/j.ultsonch.2012.07.005.Search in Google Scholar PubMed

20. Zhang, Q, Zhou, MM, Chen, PL, Cao, YY, Tan, XL. Optimization of ultrasonic-assisted enzymatic hydrolysis for the extraction of luteolin and apigenin from celery. J Food Sci 2011;76:C680–5. https://doi.org/10.1111/j.1750-3841.2011.02174.x.Search in Google Scholar PubMed

21. Turner, C, King, J W, Mathiasson, L. Supercritical fluid extraction and chromatography for fat-soluble vitamin analysis. J Chromatogr A 2001;936:215–37. https://doi.org/10.1016/s0021-9673(01)01082-2.Search in Google Scholar PubMed

22. Rehebati, N, Aytursun, A, Paiheerding, M, Wubulikasimu, A, Rustamova, N, Jingxue, C, et al. Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep). Int J Biol Macromol 2019;141:431–43. https://doi.org/10.1016/j.ijbiomac.2019.08.112.Search in Google Scholar PubMed

23. Song, JT, Feng, YX, Tian, W, Liu, J, Wang, Y, Xu, X. Enhancement of heat transfer performance using ultrasonic vaporation. Int J Food Eng 2019;15:697–711. https://doi.org/10.1515/ijfe-2018-0337.Search in Google Scholar

24. Sun, HY, Li, CY, Ni, YJ, Yao, L, Jiang, H, Ren, X, et al. Ultrasonic/microwave-assisted extraction of polysaccharides from Camptotheca acuminata fruits and its antitumor activity. Carbohydr Polym 2019;203:557–64. https://doi.org/10.1016/j.carbpol.2018.11.010.Search in Google Scholar PubMed

25. Wu, DT, Lam, SC, Cheong, KL, Wei, F, Lin, P-C, Long, Z-R, et al. Simultaneous determination of molecular weights and contents of water-soluble polysaccharides and their fractions from Lycium barbarum collected in China. J Pharmaceut Biomed Anal 2016;129:210–18. https://doi.org/10.1016/j.jpba.2016.07.005.Search in Google Scholar PubMed

26. Luo, Y, Peng, B, Liu, Y, Wu, Y, Wu, Z. Ultrasound extraction of polysaccharides from guava leaves and their antioxidant and activity. Process Biochem 2018;73:228–34. https://doi.org/10.1016/j.procbio.2018.08.003.Search in Google Scholar

27. Zhu, CP, Zhai, XC, Li, LQ, Wu, X-X, Li, B. Response surface optimization of ultrasound-assisted polysaccharides extraction from pomegranate peel. Food Chem 2015;177:139–46. https://doi.org/10.1016/j.foodchem.2015.01.022.Search in Google Scholar PubMed

28. Sin, HN, Yusof, S, Hamid, NSA, Rahman, R. Optimization of hot water extraction for sapodilla juice using response surface methodology. J Food Eng 2006;74:352–8. https://doi.org/10.1016/j.jfoodeng.2005.03.005.Search in Google Scholar

29. Ye, CL, Jiang, CJ. Optimization of extraction process of crude polysaccharides from Plantago asiatica L by response surface methodology. Carbohydr Polym 2011;84:495–502. https://doi.org/10.1016/j.carbpol.2010.12.014.Search in Google Scholar

Received: 2020-06-25
Revised: 2020-07-27
Accepted: 2020-08-12
Published Online: 2020-09-17

© 2020 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 12.11.2025 from https://www.degruyterbrill.com/document/doi/10.1515/ijfe-2020-0153/pdf
Scroll to top button