Effects of Processing Treatments on the Antioxidant Properties of Polysaccharide from Cordyceps militaris
-
Wan Chen
Abstract:
The effects of deproteinization and drying methods on the antioxidant activities and physiochemical properties of C. militaris polysaccharide were evaluated. The results showed that the IC50 of crude polysaccharide (CMP)was similar with that of deproteined polysaccharide by sevag (CMP-D-S), but significant higher (p < 0.05)than that of deprotiened polysaccharie by enzyme (CMP-D-E)on DPPH· scavenging. The IC50 of CMP was significant higher (p < 0.05)than that of CMP-D-S and CMP-D-E on OH· scavenging. Deproteinization by sevag or enzymatic method could not improve the antioxidant activity of C. militaris polysaccharide. However, drying methods influenced the antioxidant activities of C. militaris polysaccharide. Polysaccharide dried by freeze drying (CMP-F)showed respectively 55.47 % and 61.99 % higher OH· scavenging capacity on IC50 than that dried by hot air drying (CMP-H)and spray drying (CMP-S). The IC50 of CMP-F was 56.23 % higher than that of CMP-S, but similar with that of CMP-H on ABTS· scavenging. The reducing power of CMP-F was respectively 20.10 % and 27.25 % higher than that of CMP-H and CMP-S at 6 mg/mL. Spectroscopy analysis showed the dried CMPs were similar in IR and UV absorption. However, the monosaccharide ratio of CMP was changed by drying techniques. Morphological analysis showed that CMP-F consisted mainly of fluffy powder with rough and porous surface, CMP-S was spherical particle with smooth surface, and CMP-H was pyknotic and similar to anomalistic stones. Freeze drying was a recommendable method for antioxidant polysaccharide preparation from C. militaris.
Funding statement: This work was supported by Zhejiang Department of Science and Technology (Project No. 2015C32131)and Program for New Century Excellent Talents in University (Project No. NCET-13-1046).
References
1. Zhang CH, Yu Y, Liang YZ, Chen XQ. Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis. Int J Biol Macromol 2015;79:681–686.10.1016/j.ijbiomac.2015.05.060Search in Google Scholar PubMed
2. Zhao T, Mao G, Mao R, Zou Y, Zheng D, Feng W, et al Antitumor and immunomodulatory activity of a water-soluble low molecular weight polysaccharide from Schisandra chinensis (Turcz.). Baill Food Chem Toxicol 2013;55:609–616.10.1016/j.fct.2013.01.041Search in Google Scholar PubMed
3. Chen RZ, Liu ZQ, Zhao JM, Chen RP, Meng FL, Zhang M, et al Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu. Food Chem 2011;127:434–440.10.1016/j.foodchem.2010.12.143Search in Google Scholar PubMed
4. Wang L, Yu X, Yang X, Li Y, Yao Y, Lui EMK, et al Structural and anti-inflammatory characterization of a novel neutral polysaccharide from North American ginseng (Panax quinquefolius). Int J Biol Macromol 2015;74:12–17.10.1016/j.ijbiomac.2014.10.062Search in Google Scholar PubMed
5. Ye M, Chen WX, Qiu T, Yuan RY, Ye YW, Cai JM. Structural characterisation and anti-ageing activity of extracellular polysaccharide from a strain of Lachnum sp. Food Chem 2012;132:338–343.10.1016/j.foodchem.2011.10.087Search in Google Scholar PubMed
6. Ni W, Gao T, Wang H, Du Y, Li J, Li C, et al Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants. J Ethnopharmacol 2013;150:529–535.10.1016/j.jep.2013.08.055Search in Google Scholar PubMed
7. Chen X, Wu G, Huang Z. Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris. Int J Biol Macromol 2013;58:18–22.10.1016/j.ijbiomac.2013.03.041Search in Google Scholar PubMed
8. Khaskheli SG, Zheng W, Sheikh SA, Khaskheli AA, Liu Y, Soomro AH, et al Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product. Int J Biol Macromol 2015;81:387–395.10.1016/j.ijbiomac.2015.08.020Search in Google Scholar PubMed
9. Jing Y, Cui X, Chen Z, Huang L, Song L, Liu T, et al Elucidation and biological activities of a new polysaccharide from cultured Cordyceps militaris. Carbohydr Polym 2014;102:288–296.10.1016/j.carbpol.2013.11.061Search in Google Scholar PubMed
10. Fan L, Li J, Deng K, Ai L. Effects of drying methods on the antioxidant activities of polysaccharides extracted from Ganoderma lucidum. Carbohydr Polym 2012;87:1849–1854.10.1016/j.carbpol.2011.10.018Search in Google Scholar
11. Wang Y, Liu Y, Huo J, Zhao T, Ren J, Wei X. Effect of different drying methods on chemical composition and bioactivity of tea polysaccharides. Int J Biol Macromol 2013;62:714–719.10.1016/j.ijbiomac.2013.10.006Search in Google Scholar
12. Ma L, Chen H, Zhu W, Wang Z. Effect of different drying methods on physicochemical properties and antioxidant activities of polysaccharides extracted from mushroom Inonotus obliquus. Food Res Int 2013;50:633–640.10.1016/j.foodres.2011.05.005Search in Google Scholar
13. Wu S, Li F, Jia S, Ren H, Gong G, Wang Y, et al. Drying effects on the antioxidant properties of polysaccharides obtained from Agaricus blazei Murrill. Carbohydr Polym 2014;103:414–417.10.1016/j.carbpol.2013.11.075Search in Google Scholar
14. Wu Z. Effect of different drying methods on chemical composition and bioactivity of finger citron polysaccharides. Int J Biol Macromol 2015;76:218–223.10.1016/j.ijbiomac.2015.02.043Search in Google Scholar
15. Lin R, Liu H, Wu S, Pang L, Jia M, Fan K, et al. Production and in vitro antioxidant activity of exopolysaccharide by a mutant, Cordyceps militaris SU5-08. Int J Biol Macromol 2012;51:153–157.10.1016/j.ijbiomac.2012.04.011Search in Google Scholar
16. Zhang Z, Lv G, He W, Shi L, Pan H, Fan L. Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipe. Carbohydr Polym 2013;98:1524–1531.10.1016/j.carbpol.2013.07.052Search in Google Scholar
17. Shi L, Chen K, Dong Q, Fang J, Ding K. Separation, purification and structure elucidation of a polysaccharide from root of Cudrania tricuspidata. Front Chem China 2008;3:209–212.10.1007/s11458-008-0040-1Search in Google Scholar
18. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determinations of sugars and related substances. Analytical Chem 1956;28:350–356.10.1021/ac60111a017Search in Google Scholar
19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265–275.10.1016/S0021-9258(19)52451-6Search in Google Scholar
20. Wu M, Wu Y, Qu M, Li W, Yan X. Evaluation of antioxidant activities of water-soluble polysaccharides from brown alga Hizikia fusiformis. Int J Biol Macromol 2013;56:28–33.10.1016/j.ijbiomac.2013.01.017Search in Google Scholar PubMed
21. Nep EI, Conway BR. Physicochemical characterization of grewia polysaccharide gum: Effect of drying method. Carbohydr Polym 2011;84:446–453.10.1016/j.carbpol.2010.12.005Search in Google Scholar
22. Tahmouzi S, Ghodsi M. Optimum extraction of polysaccharides from motherwort leaf and its antioxidant and antimicrobial activities. Carbohydr Polym 2014;112:396–403.10.1016/j.carbpol.2014.06.024Search in Google Scholar
23. Wu H, Zhu J, Diao W, Wang C. Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin. Carbohydr Polym 2014;113:314–324.10.1016/j.carbpol.2014.07.025Search in Google Scholar
24. Wang CC, Chang SC, Inbaraj BS, Chen BH. Isolation of carotenoids, flavonoids and polysaccharides from Lycium barbarum L. and evaluation of antioxidant activity. Food Chem 2010;120:184–192.10.1016/j.foodchem.2009.10.005Search in Google Scholar
25. Zheng Y, Li Y, Wang W. Optimization of ultrasonic-assisted extraction and in vitro antioxidant activities of polysaccharides from Trametes orientalis. Carbohydr Polym 2014;111:315–323.10.1016/j.carbpol.2014.04.034Search in Google Scholar
26. Hu F, Lu R, Huang B, Ming L. Free radical scavenging activity of extracts prepared from fresh leaves of selected Chinese medicinal plants. Fitoterapia 2004;75:14–23.10.1016/j.fitote.2003.07.003Search in Google Scholar
27. Gao J, Zhang T, Jin ZY, Xu XM, Wang JH, Zha XQ, et al Structural characterisation, physicochemical properties and antioxidant activity of polysaccharide from Lilium lancifolium Thunb. Food Chem 2015;169:430–438.10.1016/j.foodchem.2014.08.016Search in Google Scholar
28. Zhao Q, Dong B, Chen J, Zhao B, Wang X, Wang L, et al Effect of drying methods on physicochemical properties and antioxidant activities of wolfberry (Lycium barbarum)polysaccharide. Carbohydr Polym 2015;127:176–181.10.1016/j.carbpol.2015.03.041Search in Google Scholar
29. Ratti C. Hot air and freeze-drying of high-value foods: a review. J Food Eng 2001;49:311–319.10.1016/S0260-8774(00)00228-4Search in Google Scholar
30. Zhao L, Dong Y, Chen G, Hu Q. Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum. Carbohydr Polym 2010;80:783–789.10.1016/j.carbpol.2009.12.029Search in Google Scholar
31. Ge Y, Duan Y, Fang G, Wang S. Polysaccharides from fruit calyx of Physalis alkekengi var. Francheti: Isolation, purification, structural features and antioxidant activities. Carbohydr Polym 2009;77:188–193.10.1016/j.carbpol.2008.12.020Search in Google Scholar
32. Kong L, Yu L, Feng T, Yin X, Liu T, Dong F. Physicochemical characterization of the polysaccharide from Bletilla striata: Effect of drying method. Carbohydr Polym 2015;125:1–8.10.1016/j.carbpol.2015.02.042Search in Google Scholar PubMed
©2017 by De Gruyter
Articles in the same Issue
- Evolutionary Algorithm-Based Multi-objective Control Scheme for Food Drying Process
- Stability and Bioaccessibility of Fucoxanthin in Nanoemulsions Prepared from Pinolenic Acid-contained Structured Lipid
- Functional and Rheological Properties of Piñuela (Bromelia karatas) in Two Ripening Stages.
- Experimental Study on Heat and Mass Transfer of Millet in a Fixed Furnace
- Effects of Processing Treatments on the Antioxidant Properties of Polysaccharide from Cordyceps militaris
- Production and Preliminary Characterization of Antioxidant Polysaccharide by Submerged Culture of Culinary and Medicinal Fungi Cordyceps militaris CICC14013
- Effect of Heat Processing and Ultrasonication Treatment on Custard Apple Peroxidase Activity and Vitamin C
- Response Surface Methodology Approach for Optimization of Extrusion Process of Production of Poly (Hydroxyl Butyrate-Co-Hydroxyvalerate) /Tapioca Starch Blends
- Phenolic Profile and Antioxidant Capacity of Walnut Extract as Influenced by the Extraction Method and Solvent
- Optimization of the Spray-Drying Process for Developing Stingless Bee Honey Powder
Articles in the same Issue
- Evolutionary Algorithm-Based Multi-objective Control Scheme for Food Drying Process
- Stability and Bioaccessibility of Fucoxanthin in Nanoemulsions Prepared from Pinolenic Acid-contained Structured Lipid
- Functional and Rheological Properties of Piñuela (Bromelia karatas) in Two Ripening Stages.
- Experimental Study on Heat and Mass Transfer of Millet in a Fixed Furnace
- Effects of Processing Treatments on the Antioxidant Properties of Polysaccharide from Cordyceps militaris
- Production and Preliminary Characterization of Antioxidant Polysaccharide by Submerged Culture of Culinary and Medicinal Fungi Cordyceps militaris CICC14013
- Effect of Heat Processing and Ultrasonication Treatment on Custard Apple Peroxidase Activity and Vitamin C
- Response Surface Methodology Approach for Optimization of Extrusion Process of Production of Poly (Hydroxyl Butyrate-Co-Hydroxyvalerate) /Tapioca Starch Blends
- Phenolic Profile and Antioxidant Capacity of Walnut Extract as Influenced by the Extraction Method and Solvent
- Optimization of the Spray-Drying Process for Developing Stingless Bee Honey Powder
