Changes in Some Quality Properties of Kefir during Storage and Inhibition Effect of Water Soluble Extracts on Angiotensin-I Converting Enzyme Purified by Human Plasma
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Tuba Erkaya
,
Aykut Öztekin
Abstract
Angiotensin converting enzyme (ACE)-inhibitory activity in water soluble extracts (WSEs) of kefir was investigated. Kefir was produced traditionally using kefir grains and stored at refrigerated temperature for 20 days. During storage period (on 1, 5, 10, 15 and 20 days) in vitro ACE-inhibitory activity in WSEs was determined. ACE was purified from human plasma to determine kinetic parameters. Purified ACE had a specific activity of 20.75 EU.mg−1, a yield of 16.6% with a factor of 22100. The inhibition effects of kefir on ACE increased at 15 storage days than other storage days. Some microbiological and physicochemical characteristics of kefir were also studied. Counts of presumptive LAB on M-17 and presumptive LAB on MRS in the kefir were about 108 CFU.ml−1 throughout the storage period. Yeast counts were lower than lactic acid bacteria counts and the average of the counts was approximately 106 log CFU.ml−1. Storage period had a significant effect (P < 0.05) on titratable acidity and pH values. On the contrary, it had no significant effect (P > 0.05) on viscosity and serum separation values of kefir.
References
1. Lopitz-Otsoa F, Rementeria A, Elguezabal N, Kefir GJ. A symbiotic yeasts-bacteria community with alleged healthy capabilities. Rev Iberoam Micol 2006;23:67–74.10.1016/S1130-1406(06)70016-XSuche in Google Scholar
2. Farnworth ER, Mainville I. Kefir: a fermented milk product. In: Farnworth ER, editor. Handbook of fermented functional foods. Florida: CRC Press, 2003:77–112.10.1201/9780203009727.ch4Suche in Google Scholar
3. Jianzhong Z, Xiaoli L, Hanhu J, Mingsheng D. Analysis of the microflora in Tibetan kefir grains using denaturing gradient gel electrophoresis. Food Microbiol 2009;26:770–5.10.1016/j.fm.2009.04.009Suche in Google Scholar
4. Vinderola G, Perdigon G, Duarte J, Thangavel D, Farnworth E, Matar C. Effects of kefir fractions on innate immunity. Immunobiol 2006;211:149–56.10.1016/j.imbio.2005.08.005Suche in Google Scholar
5. Liu JR, Lin CW. Production of kefir from soymilk with or without added glucose, lactose or sucrose. J Food Sci 2000;65:716–19.10.1111/j.1365-2621.2000.tb16078.xSuche in Google Scholar
6. Quirós A, Hernández-Ledesma B, Ramos M, Amigo L, Recio I. Angiotensin-converting enzyme inhibitory activity of peptides derived from caprine kefir. J Dairy Sci 2005;88:3480–7.10.3168/jds.S0022-0302(05)73032-0Suche in Google Scholar
7. Skeggs LT, Marsh WH, Kahn JK, Shumway NP. The existence of two forms of hypertension. J Exp Med 1954;99:275–82.10.1084/jem.99.3.275Suche in Google Scholar
8. Sieber R, Bütikofer U, Egger C, Portmann R, Walther B, Wechsler D. ACE-inhibitory activity and ACE-inhibiting peptides in different cheese varieties. Dairy Sci Technol 2010;90:47–73.10.1051/dst/2009049Suche in Google Scholar
9. Xie Y, Zhang W. Antihypertensive activity of Rosa rugosa Thunb. Flowers: Angiotensin I converting enzyme inhibitor. J Ethnopharmacol 2012;144:562–6.10.1016/j.jep.2012.09.038Suche in Google Scholar
10. Goretta LA, Ottaviani JI, Keen CL, Fraga CG. Inhibition of angiotensin converting enzyme (ACE) activity by flavan-3-ols and procyanidins. FEBS Lett 2003;555:597–600.10.1016/S0014-5793(03)01355-3Suche in Google Scholar
11. Pihlanto-Leppälä A. Bioactive peptides derived from bovine whey proteins: opioid and ace-inhibitory peptides. Trends Food Sci Tech 2000;11:347–56.10.1016/S0924-2244(01)00003-6Suche in Google Scholar
12. Li F, Yamaki K, Cheng Y, Fang Y. Assessment and Separation of angiotensin I-converting enzyme inhibitory peptides in Chinese soypaste. Int J Food Eng 2015;11:301–5.10.1515/ijfe-2014-0221Suche in Google Scholar
13. Lee DH, Kim JH, Park JS, Choi YJ, Lee JS. Isolation and characterization of a novel angiotensin-I-converting enzyme inhibitory peptide derived from the edible mushroom Tricholoma giganteum. Peptides 2004;25:621–7.10.1016/j.peptides.2004.01.015Suche in Google Scholar PubMed
14. Gobbetti M, Stepaniak L, De Angelis M, Corsetti A, Di Cagno R. Latent bioactive peptides in milk proteins: proteolytic activation and significance in dairy processing. Crit Rev Food Sci Nutr 2002;42:223–39.10.1080/10408690290825538Suche in Google Scholar PubMed
15. Erkaya T, Şengul M. Bioactivity of water soluble extracts and some characteristics of white cheese during the ripening period as effected by packaging type and probiotic adjunct cultures. J Dairy Res 2015;82:47–55.10.1017/S0022029914000703Suche in Google Scholar PubMed
16. Gobbetti M, Ferranti P, Smacchi E, Goffredi F, Addeo F. Production of angiotensin-I-converting-enzyme-inhibitory peptides in fermented milks started by lactobacillus delbrueckii subsp. bulgaricus SS1 and Lactococcus lactis subsp. cremoris FT4. Appl Environ Microbiol 2000;66:3898–904.10.1128/AEM.66.9.3898-3904.2000Suche in Google Scholar PubMed PubMed Central
17. Algaron F, Miranda G, Le Bars D, Monnet V. Milk fermentation by Lactococcus lactis with modified proteolytic systems to accumulate potentially bio-active peptides. Lait 2004;84:115–23.10.1051/lait:2003034Suche in Google Scholar
18. Ashar MN, Chand R. Antihypertensive peptides purified from milks fermented with Lactobacillus delbrueckii ssp. bulgaricus. Milchwissenschaft 2004;59:14–17.Suche in Google Scholar
19. Wang H, Zhang S, Sun Y, Dai Y. ACE-inhibitory peptide isolated from fermented soybean meal as functional food. Int J Food Eng 2013;9:1–8.10.1515/ijfe-2012-0207Suche in Google Scholar
20. Kwon YI, Apostolidis E, Shetty K. Anti-diabetes functionality of kefir culture-mediated fermented soymilk supplemented with Rhodiola extracts. Food Biotechnol 2006;20:13–29.10.1080/08905430500522055Suche in Google Scholar
21. Pihlanto A, Tarja V, Angiotensin HK. I converting enzyme (ACE) inhibitory activity and antihypertensive effect of fermented milk. Int Dairy J 2010;20:3–10.10.1016/j.idairyj.2009.07.003Suche in Google Scholar
22. Terzaghi BE, Sandine WE. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol 1975;29:807–13.10.1128/am.29.6.807-813.1975Suche in Google Scholar
23. Rogosa M, Mitchell JA, Wiseman RF. A selective medium for the isolation of oral and fecal lactobacilli. J Bacteriol 1951;62:132–3.10.1128/jb.62.1.132-133.1951Suche in Google Scholar
24. Metin M, Süt ve Mamülleri Analiz Yöntemleri. Bornova, İzmir, Turkey: Ege Üniversitesi Press, 2008:439.Suche in Google Scholar
25. IDF (International Dairy Federation), Standard Method 20B: Determination of Nitrogen Content, IDF, Brussels, Belgium, 1993.Suche in Google Scholar
26. Sabeur K, Vo AT, Ball BA. Characterization of angiotensin-converting enzyme in canine testis. Reprod 2001;122:139–46.10.1530/rep.0.1220139Suche in Google Scholar
27. Laemmli DK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1975;227:680–5.10.1038/227680a0Suche in Google Scholar
28. Chevallet M, Luche S, Rabilloud T. Silver staining of proteins in polyacrylamide gels. Nat Protoc 2006;1:1852–8.10.1038/nprot.2006.288Suche in Google Scholar
29. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–51.10.1016/0003-2697(76)90527-3Suche in Google Scholar
30. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–75.10.1016/S0021-9258(19)52451-6Suche in Google Scholar
31. Irigoyen A, Arana I, Castiella M, Torre P, Ib´a˜nez FC. Microbiological, physicochemical, and sensory characteristics of kefir during storage. Food Chem 2005;90:613–20.10.1016/j.foodchem.2004.04.021Suche in Google Scholar
32. Kilic S, Uysal H, Akbulut N, Kavas G, Chemical KH. Microbiological and sensory changes in ripening kefirs produced from starters and grains. Ziraat Fakultesi Derg 1999;36:111–18.Suche in Google Scholar
33. Wszolek M, Tamime AY, Muir DD, Barclay MNI. Properties of kefir made in Scotland and Poland using bovine, caprine and ovine milk with different starter cultures. Lebensmittel-Wissenschaft & Technologie 2001;34:251–61.10.1006/fstl.2001.0773Suche in Google Scholar
34. Guzel-Seydim Z, Wyffels JT, Seydim AC, Greene AK. Turkish kefir and kefir grains: microbial enumeration and electron microscopic observation. Int J Dairy Technol 2005;58:25–9.10.1111/j.1471-0307.2005.00177.xSuche in Google Scholar
35. Öner Z, Karahan AG, Çakmakçı ML. Effects of different milk types and starter cultures on kefir. GIDA-J. Food 2010;35:177–82.Suche in Google Scholar
36. Garrote GL, Abraham AG, De Antoni GL. Inhibitory power of kefir: the role of organic acids. J Food Protect 2000;63:364–9.10.4315/0362-028X-63.3.364Suche in Google Scholar
37. Kesenkaş H, Dinkci N, Seckin K, Kınık Ö, Gönc S, Ergönül PG, et al. Physicochemical, microbiological and sensory characteristics of soymilk kefir. Afric J Microbiol Res 2011;5:3737–46.10.5897/AJMR11.579Suche in Google Scholar
38. Bull HG, Thornberry NA, Cordes EH. Purification of angiotensin-converting enzyme from rabbit lung and human plasma by affinity chromatography. J Biol Chem 1985;260:2963–72.10.1016/S0021-9258(18)89460-1Suche in Google Scholar
39. Ong L, Shah NP. Release and identification of angiotensin-converting enzyme inhibitory peptides as influenced by ripening temperatures and probiotic adjuncts in cheddar cheeses. LWT – Food Sci Technol 2008;41:1555–66.10.1016/j.lwt.2007.11.026Suche in Google Scholar
40. Kanbak G, Uzuner K, Ol KK, Oğlakçı A, Kartkaya K, Şentürk H. Effect of kefir and low-dose aspirin on arterial blood pressure measurements and renal apoptosis in unhypertensive rats with 4 weeks salt diet. Clin Exp Hypertens 2014;36:1–8.10.3109/10641963.2013.783046Suche in Google Scholar PubMed
©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Numerical Solution of a Nonlinear Diffusion Model for Soybean Hydration with Moving Boundary
- Simulation and Performance of a Solar Drying Unit with Storage for Aromatic and Medicinal Plants
- Preparation of Nano-curcumin with Enhanced Dissolution Using Ultrasonic-Assisted Supercritical Anti-solvent Technique
- Effect of Various Concentration of β-Cyclodextrin Inclusion Complexes Containing trans-Cinnamaldehyde by Molecular Self-Assembly
- Effects of Amount of Batter in Baking Cup on Muffin Quality
- Influence of Tapioca Starch on Thermal Properties of Wheat Flour-Based Batter and Quality of Fried Battered Chicken Wingsticks
- Clarification of Date Syrup by Activated Carbon: Investigation on Kinetics, Equilibrium Isotherm, and Thermodynamics of Interactions
- Changes in Some Quality Properties of Kefir during Storage and Inhibition Effect of Water Soluble Extracts on Angiotensin-I Converting Enzyme Purified by Human Plasma
- Drying Characteristics and Assessment of Physicochemical and Microstructural Properties of Dried Culinary Banana Slices
- Multi-Objective Optimization of the Pasteurization Process of Pumpkin Cubes Packaged in Glass Jars
- Mathematical Modeling of Thin-Layer Solar Drying for Yarrow, Coriander and Hollyhock
- Shorter Communication
- Drying Kinetics of American Ginseng Slices in Thin-layer Air Impingement Dryer
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Numerical Solution of a Nonlinear Diffusion Model for Soybean Hydration with Moving Boundary
- Simulation and Performance of a Solar Drying Unit with Storage for Aromatic and Medicinal Plants
- Preparation of Nano-curcumin with Enhanced Dissolution Using Ultrasonic-Assisted Supercritical Anti-solvent Technique
- Effect of Various Concentration of β-Cyclodextrin Inclusion Complexes Containing trans-Cinnamaldehyde by Molecular Self-Assembly
- Effects of Amount of Batter in Baking Cup on Muffin Quality
- Influence of Tapioca Starch on Thermal Properties of Wheat Flour-Based Batter and Quality of Fried Battered Chicken Wingsticks
- Clarification of Date Syrup by Activated Carbon: Investigation on Kinetics, Equilibrium Isotherm, and Thermodynamics of Interactions
- Changes in Some Quality Properties of Kefir during Storage and Inhibition Effect of Water Soluble Extracts on Angiotensin-I Converting Enzyme Purified by Human Plasma
- Drying Characteristics and Assessment of Physicochemical and Microstructural Properties of Dried Culinary Banana Slices
- Multi-Objective Optimization of the Pasteurization Process of Pumpkin Cubes Packaged in Glass Jars
- Mathematical Modeling of Thin-Layer Solar Drying for Yarrow, Coriander and Hollyhock
- Shorter Communication
- Drying Kinetics of American Ginseng Slices in Thin-layer Air Impingement Dryer
