The miRNA-kallikrein axis of interaction: a new dimension in the pathogenesis of prostate cancer
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Nicole M.A. White
Abstract
Kallikrein-related peptidases (KLKs) are a family of serine proteases that were shown to be useful cancer biomarkers. KLKs have been shown to be dysregulated in prostate cancer (PCa). microRNAs (miRNAs) are short RNA nucleotides that negatively regulate gene expression and have been reportedly dysregulated in PCa. We compiled a comprehensive list of 55 miRNAs that are differentially expressed in PCa from previous microarray analysis and published literature. Target prediction analyses showed that 29 of these miRNAs are predicted to target 10 KLKs. Eight of these miRNAs were predicted to target more than one KLK. Quantitative real-time (qRT)-PCR demonstrated that there was an inverse correlation pattern in the expression (normal vs. cancer) between dysregulated miRNAs and their target KLKs. In addition, we experientially validated the miRNA-KLK interaction by transfecting miR-331-3p and miR-143 into a PCa cell line. Decreased expression of targets KLK4 and KLK10, respectively, and decreased cellular growth were observed. In addition to KLKs, dysregulated miRNAs were predicted to target other genes involved in the pathogenesis of PCa. These data show that miRNAs can contribute to KLK regulation in PCa. The miRNA-KLK axis of interaction projects a new element in the pathogenesis of PCa that may have therapeutic implications.
©2012 by Walter de Gruyter Berlin Boston
Articles in the same Issue
- Guest Editorial
- Highlight: The 4th International Symposium on Kallikreins and Kallikrein-Related Peptidases
- Kallikrein-related peptidases in prostate, breast, and ovarian cancers: from pathobiology to clinical relevance
- Epigenetic regulation of kallikrein-related peptidases: there is a whole new world out there
- Non-combinatorial library screening reveals subsite cooperativity and identifies new high-efficiency substrates for kallikrein-related peptidase 14
- One round of SELEX for the generation of DNA aptamers directed against KLK6
- Kallikrein 6 is a novel molecular trigger of reactive astrogliosis
- Characterization of SPINK9, a KLK5-specific inhibitor expressed in palmo-plantar epidermis
- The miRNA-kallikrein axis of interaction: a new dimension in the pathogenesis of prostate cancer
- Stromal cell-associated expression of kallikrein-related peptidase 6 (KLK6) indicates poor prognosis of ovarian cancer patients
- The kallikrein 14 gene is down-regulated by androgen receptor signalling and harbours genetic variation that is associated with prostate tumour aggressiveness
- Kallikrein-related peptidase signaling in colon carcinoma cells: targeting proteinase-activated receptors
- Proteinase-activated receptors (PARs): differential signalling by kallikrein-related peptidases KLK8 and KLK14
- Masthead
- Masthead
Articles in the same Issue
- Guest Editorial
- Highlight: The 4th International Symposium on Kallikreins and Kallikrein-Related Peptidases
- Kallikrein-related peptidases in prostate, breast, and ovarian cancers: from pathobiology to clinical relevance
- Epigenetic regulation of kallikrein-related peptidases: there is a whole new world out there
- Non-combinatorial library screening reveals subsite cooperativity and identifies new high-efficiency substrates for kallikrein-related peptidase 14
- One round of SELEX for the generation of DNA aptamers directed against KLK6
- Kallikrein 6 is a novel molecular trigger of reactive astrogliosis
- Characterization of SPINK9, a KLK5-specific inhibitor expressed in palmo-plantar epidermis
- The miRNA-kallikrein axis of interaction: a new dimension in the pathogenesis of prostate cancer
- Stromal cell-associated expression of kallikrein-related peptidase 6 (KLK6) indicates poor prognosis of ovarian cancer patients
- The kallikrein 14 gene is down-regulated by androgen receptor signalling and harbours genetic variation that is associated with prostate tumour aggressiveness
- Kallikrein-related peptidase signaling in colon carcinoma cells: targeting proteinase-activated receptors
- Proteinase-activated receptors (PARs): differential signalling by kallikrein-related peptidases KLK8 and KLK14
- Masthead
- Masthead
