Investigation of eucalypt and pine wood acid-soluble lignin by Py-GC-MS
-
Gabriel Castro Brumano
and
Fernando José Borges Gomes
Abstract
The Klason method is adequate for quantifying lignin in softwood (SWD) but is less so for hardwood (HWD). The latter contains a fraction of acid-soluble lignin (ASL) that is not measurable under the conditions prevailing in the Klason method and that must be quantified by other means. This study aimed at investigating the composition of ASL obtained from eucalypt and pine woods by using pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS). About 2.7% and 0.9% ASL by dry wood weight were measured in the acid filtrate of eucalypt and pine, respectively, by the Technical Association of the Pulp and Paper Industry (TAPPI) UM 250 method. The acid filtrate was lyophilized and processed by Py-GC-MS, allowing identification of 26 primary pyrolysis products, with 2% and 52% relative molar abundance coming from lignin and carbohydrates, respectively, for eucalypt and 24 primary pyrolysis products, with 1% and 54% relative molar abundance from lignin and carbohydrates, respectively, for pine. The main products derived from lignin were phenol, 4-methylphenol, guaiacol, 4-methylcatechol, syringol, acetosyringone and vanillin. It was concluded that measurements of ASL remain a requirement for accurate eucalypt and pine wood lignin quantification because lignin derivatives remain in the acid filtrate from the Klason lignin quantification method. Corrections for carbohydrate-derived compounds are not necessary, despite their predominance in the filtrate, because these derivatives do not interfere with ultraviolet (UV) absorption.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Financial support provided by the Brazilian Agencies CAPES, CNPq and FAPEMIG is gratefully acknowledged.
Employment or leadership: None declared.
Honorarium: None declared.
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©2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Editorial
- The 15th European Workshop on Lignocellulosics and Pulp (EWLP) in Aveiro, Portugal (June 26–29, 2018)
- Review
- Extractives and biological activities of Lamiaceae species growing in Uzbekistan
- Original Articles
- New drum-chipping technology for a more uniform size distribution of wood chips
- Characterization of enzyme-resistant xylooligosaccharides extracted from hardwood chips by pre-hydrolysis and further depolymerized by enzymatic treatment
- Stabilising mannose using sodium dithionite at alkaline conditions
- Xylan accessibility of bleached eucalypt pulp in alkaline solutions
- Investigation of eucalypt and pine wood acid-soluble lignin by Py-GC-MS
- The reaction of lignin model compounds during enzymatic bleaching with a Curvularia verruculosa haloperoxidase: impact on chlorination
- Molecular weight-based fractionation of lignin oils by membrane separation technology
- Phenol-formaldehyde resins with suitable bonding strength synthesized from “less-reactive” hardwood lignin fractions
- Impact of birch xylan composition and structure on film formation and properties
- Gram-scale economical synthesis of trans-coniferyl alcohol and its corresponding thiol
- Lignosulfonate-based polyurethane materials via cyclic carbonates: preparation and characterization
- Bioconversion of pine stumps to ethanol: pretreatment and simultaneous saccharification and fermentation
- Selective recovery of polyphenols from MDF process waters by adsorption on a macroporous, cross-linked pyrrolidone-based resin
- Short Note
- Lignin analysis with benchtop NMR spectroscopy
Articles in the same Issue
- Frontmatter
- Editorial
- The 15th European Workshop on Lignocellulosics and Pulp (EWLP) in Aveiro, Portugal (June 26–29, 2018)
- Review
- Extractives and biological activities of Lamiaceae species growing in Uzbekistan
- Original Articles
- New drum-chipping technology for a more uniform size distribution of wood chips
- Characterization of enzyme-resistant xylooligosaccharides extracted from hardwood chips by pre-hydrolysis and further depolymerized by enzymatic treatment
- Stabilising mannose using sodium dithionite at alkaline conditions
- Xylan accessibility of bleached eucalypt pulp in alkaline solutions
- Investigation of eucalypt and pine wood acid-soluble lignin by Py-GC-MS
- The reaction of lignin model compounds during enzymatic bleaching with a Curvularia verruculosa haloperoxidase: impact on chlorination
- Molecular weight-based fractionation of lignin oils by membrane separation technology
- Phenol-formaldehyde resins with suitable bonding strength synthesized from “less-reactive” hardwood lignin fractions
- Impact of birch xylan composition and structure on film formation and properties
- Gram-scale economical synthesis of trans-coniferyl alcohol and its corresponding thiol
- Lignosulfonate-based polyurethane materials via cyclic carbonates: preparation and characterization
- Bioconversion of pine stumps to ethanol: pretreatment and simultaneous saccharification and fermentation
- Selective recovery of polyphenols from MDF process waters by adsorption on a macroporous, cross-linked pyrrolidone-based resin
- Short Note
- Lignin analysis with benchtop NMR spectroscopy
