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A matrix-resistant HPTLC method to quantify monosaccharides in wood-based lignocellulose biorefinery streams

  • Josua Timotheus Oberlerchner , Stefan Böhmdorfer , Thomas Rosenau and Antje Potthast EMAIL logo
Published/Copyright: April 25, 2018
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Holzforschung
From the journal Holzforschung Volume 72 Issue 8

Abstract

A new and robust high performance thin-layer chromatography (HPTLC) method has been developed for carbohydrate analysis in biorefinery streams. The samples can be applied directly from industrial streams without any purification or pretreatment, saving time and resources and avoiding potential alterations to the samples’ composition. The main neutral monosaccharides found in wood-based lignocellulosic biomass – glucose, xylose, mannose, arabinose and galactose – can be separated and quantified using acetonitrile:1-pentanol:water (4:1:1, v/v/v) on silica impregnated with a phosphate buffer. Common minor compounds – glucuronic acid, galacturonic acid, rhamnose, cellobiose and hydroxymethylfurfural (HMF) – can also be detected qualitatively. Matrix compounds, which are a major part of biomass, do not interfere with the analysis. Validation showed that the method is selective, has good repeatability and has limits of detection and quantification in the nanogram range. The visual fingerprint offers information on the samples’ constituents in the case of unknown samples. Also, incomplete hydrolysis is indicated by the visibility of oligosaccharides.

Keywords: biorefinery; carbohydrates; high performance thin layer chromatography; hydrolysis; matrix effects; sample preparation

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

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Article note:

Selected paper from the 19th ISWFPC – International Symposium on Wood, Fibre and Pulping Chemistry, held from August 30 to September 1st, in Porto Seguro, Brazil.

Received: 2017-10-24
Accepted: 2018-03-19
Published Online: 2018-04-25
Published in Print: 2018-07-26

©2018 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Selected paper from the 19th ISWFPC - International Symposium on Wood, Fibre and Pulping Chemistry, held in Porto Seguro, Brazil, August 30–September 1
  3. Modeling kraft cooking kinetics of fiber mixes from TMP and unbleached kraft pulps for assessment of old corrugated cardboard delignification
  4. Effect of autohydrolysis on alkaline delignification of mixed hardwood chips and on lignin structure
  5. Lignin-based coatings for controlled P-release fertilizer consisting of granulated simple superphosphate
  6. A matrix-resistant HPTLC method to quantify monosaccharides in wood-based lignocellulose biorefinery streams
  7. Review
  8. Utilization and characterization of amino resins for the production of wood-based panels with emphasis on particleboards (PB) and medium density fibreboards (MDF). A review
  9. Original Articles
  10. Influence of veneer thickness, mat formation and resin content on some properties of novel poplar scrimbers
  11. Low-velocity impact response of wood-strand sandwich panels and their components
  12. Interactions between PLA, PE and wood flour: effects of compatibilizing agents and ionic liquids
  13. Industrial Thermowood® and Termovuoto thermal modification of two hardwoods from Mozambique
  14. Boron fixation effect of quaternary ammonium compounds (QACs) on sodium fluoroborate (NaBF4)-treated wood
https://doi.org/10.1515/hf-2017-0170
Keywords for this article
biorefinery; carbohydrates; high performance thin layer chromatography; hydrolysis; matrix effects; sample preparation

Articles in the same Issue

  1. Frontmatter
  2. Selected paper from the 19th ISWFPC - International Symposium on Wood, Fibre and Pulping Chemistry, held in Porto Seguro, Brazil, August 30–September 1
  3. Modeling kraft cooking kinetics of fiber mixes from TMP and unbleached kraft pulps for assessment of old corrugated cardboard delignification
  4. Effect of autohydrolysis on alkaline delignification of mixed hardwood chips and on lignin structure
  5. Lignin-based coatings for controlled P-release fertilizer consisting of granulated simple superphosphate
  6. A matrix-resistant HPTLC method to quantify monosaccharides in wood-based lignocellulose biorefinery streams
  7. Review
  8. Utilization and characterization of amino resins for the production of wood-based panels with emphasis on particleboards (PB) and medium density fibreboards (MDF). A review
  9. Original Articles
  10. Influence of veneer thickness, mat formation and resin content on some properties of novel poplar scrimbers
  11. Low-velocity impact response of wood-strand sandwich panels and their components
  12. Interactions between PLA, PE and wood flour: effects of compatibilizing agents and ionic liquids
  13. Industrial Thermowood® and Termovuoto thermal modification of two hardwoods from Mozambique
  14. Boron fixation effect of quaternary ammonium compounds (QACs) on sodium fluoroborate (NaBF4)-treated wood
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