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Microbial xylitol production from culm of Sasa kurilensis using the yeast Candida magnoliae

  • Masahiro Miura , Tomoaki Seo , Yasutaka Shimotori , Masakazu Aoyama EMAIL logo , Hisayuki Nakatani and Masatomo Nishikoori
Published/Copyright: September 4, 2013
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Holzforschung
From the journal Holzforschung Volume 67 Issue 8

Abstract

A sugar solution containing 31 g l-1 xylose was prepared from the culm of Sasa kurilensis by hydrolysis with 2% sulfuric acid with a liquor-to-solid ratio of 6 (g g-1) at 121°C for 1 h. During acid hydrolysis, also some byproducts were generated, such as acetic acid, furfural, 5-hydroxymethylfurfral, and low molecular weight phenolics, which inhibit bioconversion of xylose to xylitol. Except for acetic acid, these inhibitors were successfully removed from the hydrolysate by contacting with a steam-activated charcoal (15 g l-1 dose) for 24 h. Bioconversion of the detoxified hydrolysate to xylitol by the yeast, Candida magnoliae, was investigated under various microaerobic conditions. The oxygen transfer rate (OTR) varied from 8.4 to 27.6 mmol-O2 l-1 h-1. The maximum xylitol yield (0.62 g-xylitol g-xylose-1) was attained at the OTR of 1.2 mmol-O2 l-1 h-1. An additional increase in the OTR brought about cell growth, which consumed xylose. A proper control of the oxygen supply is necessary to produce efficiently xylitol from the culm hydrolysate.

Keywords: bamboo grass; Candida magnoliae; hemicellulose hydrolysate; Sasa kurilensis; xylitol
Corresponding author: Masakazu Aoyama, Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Japan, e-mail:

The authors would like to thank Mr. Christopher Bozek, Kitami Institute of Technology, for revising this paper.

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Received: 2013-3-14
Accepted: 2013-7-12
Published Online: 2013-09-04
Published in Print: 2013-12-01

©2013 by Walter de Gruyter Berlin Boston

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Original Articles
  4. Multi-step degradation method for β-O-4 linkages in lignins: γ-TTSA method. Part 3. Degradation of milled wood lignin (MWL) from Eucalyptus globulus
  5. Evaluation of selective extraction methods for recovery of polyphenols from pine
  6. Isocyanate-treated cellulose pulp and its effect on the alkali resistance and performance of fiber cement composites
  7. Hemicelluloses extraction from giant bamboo (Bambusa balcooa Roxburgh) prior to kraft or soda-AQ pulping and its effect on pulp physical properties
  8. Effect of hydrothermal treatment intensity on the formation of degradation products from birchwood
  9. Short-time ultrasonication treatment in enzymatic hydrolysis of biomass
  10. Esterified lignin coating as water vapor and oxygen barrier for fiber-based packaging
  11. Storage-induced emissions from different wood species
  12. Determination of cohesive laws in wood bonded joints under mode I loading using the DCB test
  13. Production of furans from hemicellulosic saccharides in biphasic reaction systems
  14. Chemistry and water-repelling properties of phenyl-incorporating wood composites
  15. Comparison of results obtained by static 3- and 4-point bending and flexural vibration tests on solid wood, MDF, and 5-plywood
  16. Delamination detection in a 90-year-old glulam block with scanning dry point-contact ultrasound
  17. Short Notes
  18. Microbial xylitol production from culm of Sasa kurilensis using the yeast Candida magnoliae
  19. Determination of lignin content in kraft black liquors by capillary zone electrophoresis (CZE)
  20. Meetings
  21. Meetings
https://doi.org/10.1515/hf-2013-0040
Keywords for this article
bamboo grass; Candida magnoliae; hemicellulose hydrolysate; Sasa kurilensis; xylitol

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Original Articles
  4. Multi-step degradation method for β-O-4 linkages in lignins: γ-TTSA method. Part 3. Degradation of milled wood lignin (MWL) from Eucalyptus globulus
  5. Evaluation of selective extraction methods for recovery of polyphenols from pine
  6. Isocyanate-treated cellulose pulp and its effect on the alkali resistance and performance of fiber cement composites
  7. Hemicelluloses extraction from giant bamboo (Bambusa balcooa Roxburgh) prior to kraft or soda-AQ pulping and its effect on pulp physical properties
  8. Effect of hydrothermal treatment intensity on the formation of degradation products from birchwood
  9. Short-time ultrasonication treatment in enzymatic hydrolysis of biomass
  10. Esterified lignin coating as water vapor and oxygen barrier for fiber-based packaging
  11. Storage-induced emissions from different wood species
  12. Determination of cohesive laws in wood bonded joints under mode I loading using the DCB test
  13. Production of furans from hemicellulosic saccharides in biphasic reaction systems
  14. Chemistry and water-repelling properties of phenyl-incorporating wood composites
  15. Comparison of results obtained by static 3- and 4-point bending and flexural vibration tests on solid wood, MDF, and 5-plywood
  16. Delamination detection in a 90-year-old glulam block with scanning dry point-contact ultrasound
  17. Short Notes
  18. Microbial xylitol production from culm of Sasa kurilensis using the yeast Candida magnoliae
  19. Determination of lignin content in kraft black liquors by capillary zone electrophoresis (CZE)
  20. Meetings
  21. Meetings
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