Intergeneric hybidization of sunflower (Helianthus annuus L.) with spiny plumeless thistle (Carduus acanthoides L.)
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Miroslava Hristova-Cherbadzhi
Abstract
Carduus acanthoides L (spiny plumeless thistle) a biannual wild species with 2n = 22 chromosomes was crossed with Helianthus annuus L. When crossing, pollen from C. acanthoides germinated and pollinated the stigmas of sunflower lines HA 89A after 48 h. The crossability rate was low, but seeds and hybrid plants were obtained. The F1 plants strongly resembled the cultivated sunflower with the most important bio-morphological characters, even though they had an intermediate type of heritability. The hybrid nature was confirmed by RAPD markers. The polymorphism between H. annuus, C. acanthoides, and their F1 hybrids was studied using RAPD. The result showed introgression of C. acanthoides in the hybrid progeny. It was established that the wild species carried Rf genes for the CMS PET-1. After self-pollination and sib-pollination of the F1 plants and back-crossing with cultivated sunflower, F2, BC1 and next generation hybrid progenies were obtained. The investigation encompassed the period 2000–2007 and 2014–2018. Some of the new lines have been included in a heterosis breeding program for developing hybrids for the sunflower market.
Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Mihsan.
Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
References
Atlagić, J., Hristov, M., and Škorić, D. (1997). Cytogenetic study of the intergeneric sunflower hybrid Helianthus annuus var. cultus L. × Tithonia rotundifolia Blake (Helianthus × Tithonia). In: Proceedings of 38th conference “production and processing of oilseeds”, 16–21 June 1997, Budva, Yugoslavia, pp. 124–131.Search in Google Scholar
Blake, S.F. (1918). A revision of the genus Viguiera. Gray Herbarium Contrib, 54, pp. 1–205.10.5962/p.336015Search in Google Scholar
Christov, M. (1992). Species of Helianthus and Tithonia sources of Rf genes for CMS in sunflower. In: Proceedings 13th international sunflower conference, pp. 1356–1361, Pisa, Italy.Search in Google Scholar
Christov, M. (1998). Hybridization between cultivated sunflower (Helianthus annuus L.) and species of genus Verbesinia L. In: XVth international congress on sexual plant reproduction, Wageningen, 16-21. 08. 1988.Search in Google Scholar
Christov, M. (1999). Report of the FAO working group, evaluation and use of wild Helianthus species. Helia 22, special issue.Search in Google Scholar
Christov, M. (2000). Production of intergeneric hybrids in Compositae. Vortr. Pflanzenzüchtg 47: 43.Search in Google Scholar
Christov, M. (2001a). Intergeneric hybrids production in Compositae. Res. Commun. of USB, Dobrich 3: 28–31.Search in Google Scholar
Christov, M. (2001b). Report of the FAO working group, evaluation and use of wild Helianthus species grown in the collection of Dobroudja Agricultural Institute, general Toshevo, Bulgaria for the period 1999-2000. In: Sieler, G.J (Ed.), FAO working group, evaluation and use of wild helianthus species, progress report 1999–2000, pp. 29–31.Search in Google Scholar
Christov, M. (2013). Contribution of interspecific and intergeneric hybridization to the sunflower breeding. Helia 36: 1–18, https://doi.org/10.2298/hel1358001a.Search in Google Scholar
Christov, M. and Panajotov, I. (1991). Hybrids between the genera Helianthus and Tithonia and their study. Helia 14: 27–34.Search in Google Scholar
Christov, M. and Vassilevska-Ivanova, R.D. (1999). Intergeneric hybrids in Compositae (Asteraceae). I. Hybridization between cultivated sunflower H. annuus L. and Compositae genera. Helia 22: 13–22.Search in Google Scholar
Christov, M., Vasileva, R., Tsujimoto, H., and Panajotov, I. (1994). Intergeneric hybridization between sunflower and some species of generas from Compositae. In: International compositae conference, pp. 80, Royal Botanic Gardens, Kew, 26.07-05.08.Search in Google Scholar
Christov, M., Kiryakov, I., Shindrova, P., Encheva, V., and Christova, M. (2004). Evaluation of new interspecific and intergeneric sunflower hybrids for resistance to Sclerotinia sclerotiorum. In: Proceedings of the 16th international sunflower conference, Fargo, North Dakota, USA, August 29–September 2, 2004, II, pp. 693–699.Search in Google Scholar
Dellaporta, S.L., Wood, Y., and Hick, Y.B. (1983). Plant Mol. Biol. Rep. 4: 419–421.Search in Google Scholar
Encheva, J. and Christov, M. (2005). Intergeneric hybrids between cultivated sunflower (Helianthus annuus L.) and Verbisina helianthoides (genus Verbisina) - morphological and biochemical aspects. Helia 28: 27–36, https://doi.org/10.2298/hel0542027e.Search in Google Scholar
Encheva, V. and Kiryakov, I. (2002). Method for evaluation of sunflower resistance for Diaporthe (Phomopsis) helianthi Munt. Cnet. et al. Bulg. J. Agric. Sci. 8: 219–222.Search in Google Scholar
Encheva, J., Christov, M., and Ivanov, P. (2003). Characterization of self-fertile lines, produced by hybridization between cultivated sunflower (H. annuus L.) and distant species from the genera Helianthus and Verbisina. Bulg. J. Agric. Sci. 9: 23–28.Search in Google Scholar
Encheva, J., Köhler, H., Christov, M., and Friedt, W. (2005). Intergeneric hybrids between cultivated sunflower (Helianthus annuus L.) and Verbisina helianthoides (genus Verbisina) - RAPD analysis. Helia 28: 37–44, https://doi.org/10.2298/hel0542037e.Search in Google Scholar
Gómez-Martínez, M., and Humberto Reyes-Valdés, M. (2016). IRREGULARIDADES MEIÓTICAS EN HÍBRIDOS INTERGENÉRICOS Helianthus annuus L. x Tithonia rotundifolia (Mill.) S.F. Blake. Rev. Fitotec. Mex. 39: 117–121, https://doi.org/10.35196/rfm.2016.2.117-121.Search in Google Scholar
Heiser, C.B. (1963). Artificial intergeneric hybrids of Helianthus and Viguiera. Madrono 17: 118–127.Search in Google Scholar
Heiser, C.B., Smith, D.M., Clevengern, S.B., and Martin, W.C. (1969). The North American sunflower (Helianthus). Mem. Torrey Bot. Club 22: 1–218.Search in Google Scholar
Hristova-Cherbadzhi, M. (2004). Photosynthesis in Helianthus annuus (2n=34) x Carduus acanthoides (2n=22). Parameters of chlorophyll fluorescence and plastid pigments concentration. Field Crop. Stud. I: 246–254.Search in Google Scholar
Kho, Y.O. and Bäer, J. (1968). Observing pollen tubes by means of fluorescence. Euphytica 17: 298–302.10.1007/BF00021224Search in Google Scholar
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature 227: 680–685, https://doi.org/21910.1038/227680a0.10.1038/227680a0Search in Google Scholar PubMed
Luévanos–Escareño, M.P., Reyes–Valdés, M.H, Villarreal–Quintanilla, J.Á., and Rodríguez–Herrera, R. (2010). Obtention of intergeneric hybrids Helianthus annuus x Tithonia rotundifolia and their morphological and molecular analysis. Acta Bot. Mex.: 0187–7151.10.21829/abm90.2010.300Search in Google Scholar
Morozov, V.C. (1947). Breeding of sunflower in SSSR. Pishtepromizdat, Moskva, p. 272.[In Rus.].Search in Google Scholar
Owczarzak, A. (1952). A rapid method for mounting pollen grains: with special regard to sterility studies. Stain Technol. 27: 249, https://doi.org/21910.3109/10520295209105085.10.3109/10520295209105085Search in Google Scholar PubMed
Reyes-Valdés, H.M., Gómez-Martínez, M., Martínez, O., and Godínez, F.H. (2005). Intergeneric hybrid between cultivated sunflower (Helianthus annuus L.) and Tithonia rotundifia (Mill.) Blake. Helia 28: 61–68. https://doi.org/10.2298/hel0543061r.Search in Google Scholar
Vassilevska-Ivanova, R. (2005). A short-cycle sunflower line derived from intergeneric hybridization Helianthus x Verbesinia. Helia 28: 55–60, https://doi.org/21910.2298/hel0542051v.10.2298/HEL0543055VSearch in Google Scholar
Vassilevska-Ivanova, R. and Tcekova, Z. (2002). An attempt for hybridization Helianthus x Verbesina. Comptes rendus de l’Académie bulgare des Sciences 55: 99–104.Search in Google Scholar
Vassilevska-Ivanova, R., Christov, M., and Lidanski, T. (1996). A new type of flowers in sunflower inflorescence by crosses between Helianthus annuus L. and Verbesina helianthoides Michaux. C. R. Acad. Bulg. Sci. 49: 91–92.Search in Google Scholar
Vassilevska-Ivanova, R., Tcekova, Z., and Lidanski, T. (1999). Intergeneric hybridization in cultivated sunflower H. annuus L. and Tithonia rotundifia Blake. C. R. Acad. Bulg. Sci. 52: 93–96.Search in Google Scholar
Vear, F. and Tourvieille, D. (1987). Test de resistance au Mildiou chez le tournesol. CETIOM. Inf Tech 98: 19–20.Search in Google Scholar
Vladova, R., Petrova, S., Gerasimov, R., and Nikolov, C. (1989). Electrophoretic analysis of storageproteins in soybean (Glycine max L. Merril) cultivars and mutant lines. Genet. Sel. 22: 106–110, [In Bulg.].Search in Google Scholar
© 2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Analysis of genetic determination of partial resistance to white rot in sunflower
- Assessment of Mating System in Helianthus annuus and H. petiolaris (Asteraceae) Populations
- Stability some sunflower genotypes across divergent environments
- Automatic Phenotyping Test of Sunflower Seeds
- Agronomic attributes and stability of exotic sunflower hybrids in Iran
- Intergeneric hybidization of sunflower (Helianthus annuus L.) with spiny plumeless thistle (Carduus acanthoides L.)
- Response of sunflower hybrids to crop density in the steppe of Ukraine
Articles in the same Issue
- Frontmatter
- Analysis of genetic determination of partial resistance to white rot in sunflower
- Assessment of Mating System in Helianthus annuus and H. petiolaris (Asteraceae) Populations
- Stability some sunflower genotypes across divergent environments
- Automatic Phenotyping Test of Sunflower Seeds
- Agronomic attributes and stability of exotic sunflower hybrids in Iran
- Intergeneric hybidization of sunflower (Helianthus annuus L.) with spiny plumeless thistle (Carduus acanthoides L.)
- Response of sunflower hybrids to crop density in the steppe of Ukraine
