Involvement of MTHFR and TPMT genes in susceptibility to childhood acute lymphoblastic leukemia (ALL) in Mexicans
-
Ossyneidee Gutiérrez-Álvarez
,
Carlos Galaviz-Hernández
Abstract
Background: Folate metabolism plays an essential role in the processes of DNA synthesis and methylation. Deviations in the folate flux resulting from single-nucleotide polymorphisms in genes encoding folate-dependent enzymes may affect the susceptibility to leukemia. This case-control study aimed to assess associations among MTHFR (C677T, A1298C) and TPMT (*2, *3A) mutations as well as to evaluate the synergistic effects of combined genotypes for both genes. Therefore, these genetic variants may lead to childhood acute lymphoblastic leukemia (ALL) susceptibility, in a Mexican population study.
Methods: DNA samples obtained from 70 children with ALL and 152 age-matched controls (range, 1–15 years) were analyzed by real-time reverse transcription polymerase chain reaction (RT-qPCR) to detect MTHFR C677T and A1298C and TPMT*2 and TPMT*3A genotypes.
Results: The frequency of the MTHFR A1298C CC genotype was statistically significant (odds ratio [OR], 6.48; 95% 95% confidence intervals [CI], 1.26–33.2; p=0.025). In addition, the combined 677CC+1298AC genotype exhibited a statistically significant result (OR, 0.23; 95% CI, 0.06–0.82; p=0.023). No significant results were obtained from the MTHFR (C677T CT, C677T TT) or TPMT (*2, *3A) genotypes. More importantly, no association between the synergistic effects of either gene (MTHFR and/or TPMT) and susceptibility to ALL was found.
Conclusions: The MTHFR A1298C CC genotype was associated with an increased risk of developing childhood ALL. However, a decreased risk to ALL with the combination of MTHFR 677CC+1298AC genotypes was found.
Acknowledgments
We are grateful to Jehová-Nissi who made the study possible. We are grateful to Maria Cristina Venzor Sanchez from El Centro Estatal de Cancerología del Estado de Durango for her assistance with sample collection. Additionally, we offer our gratitude to CONACYT for the postgraduate scholarship provided to O.G.-A. at CIIDIR-Durango IPN.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Editorial
- The role of pharmacogenetics and pharmacogenomics in 21st-century medicine: state of the art and new challenges discussed in the VII Conference of the Spanish Pharmacogenetics and Pharmacogenomics Society (SEFF)
- Mini Reviews
- Human genetics: international projects and personalized medicine
- Clinical implementation of pharmacogenetics
- Progress in pharmacogenetics: consortiums and new strategies
- Pharmacogenetics and pharmacogenomics as tools in cancer therapy
- Original Articles
- Metabolism and metabolite profiles in vitro and in vivo of ospemifene in humans and preclinical species
- Involvement of MTHFR and TPMT genes in susceptibility to childhood acute lymphoblastic leukemia (ALL) in Mexicans
- Garlic capsule and selenium-vitamins ACE combination therapy modulate key antioxidant proteins and cellular adenosine triphosphate in lisinopril-induced lung damage in rats
- Case Report
- Severe verapamil intoxication despite correct use of low-dose verapamil
- Congress Abstracts
- VII Conference of the Spanish Pharmacogenetics and Pharmacogenomics Society (SEFF). “The role of pharmacogenetics and pharmacogenomics in the XXI century medicine: current state of the art and new challenges”
Articles in the same Issue
- Frontmatter
- Editorial
- The role of pharmacogenetics and pharmacogenomics in 21st-century medicine: state of the art and new challenges discussed in the VII Conference of the Spanish Pharmacogenetics and Pharmacogenomics Society (SEFF)
- Mini Reviews
- Human genetics: international projects and personalized medicine
- Clinical implementation of pharmacogenetics
- Progress in pharmacogenetics: consortiums and new strategies
- Pharmacogenetics and pharmacogenomics as tools in cancer therapy
- Original Articles
- Metabolism and metabolite profiles in vitro and in vivo of ospemifene in humans and preclinical species
- Involvement of MTHFR and TPMT genes in susceptibility to childhood acute lymphoblastic leukemia (ALL) in Mexicans
- Garlic capsule and selenium-vitamins ACE combination therapy modulate key antioxidant proteins and cellular adenosine triphosphate in lisinopril-induced lung damage in rats
- Case Report
- Severe verapamil intoxication despite correct use of low-dose verapamil
- Congress Abstracts
- VII Conference of the Spanish Pharmacogenetics and Pharmacogenomics Society (SEFF). “The role of pharmacogenetics and pharmacogenomics in the XXI century medicine: current state of the art and new challenges”
