Seasonal variation in alkaloid composition and antiproliferative activity of Stylophorum lasiocarpum (Oliv.) Fedde
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Kristýna Šebrlová
,
Ondřej Peš
Abstract
Stylophorum lasiocarpum (Oliv.) Fedde (Papaveraceae) belongs to traditional Chinese medicine herbs but there was minimal information on the content of alkaloids in this plant. Extracts from the aerial part and roots were examined by liquid chromatography with UV and mass spectrometric detection, with nineteen alkaloids identified. Changes in alkaloid content over the entire vegetation period of a one- and two-year old plant were studied. The protoberberine alkaloids, coptisine and stylopine, were found to be the main substances in extracts of the aerial part irrespective of the plant’s age and time of harvest. Variable amounts of protopine, sanguinarine, chelerythrine, chelirubine, macarpine, chelilutine and berberine were also recorded in the aerial part. The roots contained significantly larger quantities of all alkaloids than the aerial part with the levels of most alkaloids varying from May to October, peaking in the middle of the vegetation period. Coptisine was the dominant alkaloid in all samples. The antiproliferative activities of the root extract and of seven individual alkaloids were tested on A375 human malignant melanoma cells. The significant dose-dependent toxicity of the root extract was attributed largely to the quaternary benzo[c]phenanthridine alkaloids, macarpine and sanguinarine.
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Articles in the same Issue
- Perovskite ceramics and recent experimental progress in reactor design for chemical looping combustion application
- Total reflection X-ray fluorescence analysis of fly ash from Bulgarian coal-fired power plants
- Ascorbic acid amperometric sensor using a graphene-wrapped hierarchical TiO2 nanocomposite
- Rapid determination of products of phenol hydrogenation in a supercritical water system using headspace gas chromatography
- Swelling of N-vinylcaprolactam–dodecyl methacrylate gel in {heptane + toluene} mixtures
- Extraction of Cu(II) with Acorga M5640 using hollow fibre liquid membrane
- Heat transfer in a jacketed agitated vessel equipped with multistage impellers
- Seasonal variation in alkaloid composition and antiproliferative activity of Stylophorum lasiocarpum (Oliv.) Fedde
- A polyacrylate prepared using polyurethane surfactants: properties of emulsion and film
- Efficient synthesis of glutaric acid from L-glutamic acid via diazoniation/hydrogenation sequence
- New insights into bromination process: effective preparation of Ambroxol
- A concise route to 4-aminomethylpyrazoles and 4-aminomethylisoxazoles from acetylacetone-derived hexahydropyrimidines under mild conditions
- Assessment of non-standard reaction conditions for asymmetric 1,3-dipolar organocatalytic cycloaddition of nitrone with α,β-unsaturated aldehydes
- β-Cyclodextrin as water-solubility enhancer for butylated hydroxytoluene
- Asymmetric polymerisation of substituted phenylacetylene using chiral Rh(2,5-norbornadiene)(L-proline) catalyst
