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Exercise-induced changes in miRNA expression in coronary artery disease

  • Barbara Mayr ORCID logo , Edith E. Müller , Christine Schäfer , Silke Droese , Martin Schönfelder and Josef Niebauer EMAIL logo
Published/Copyright: May 12, 2021
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 59 Issue 10

Abstract

Objectives

Micro ribonucleic acids (miRNAs) are small non-coding RNA molecules that control gene expression by translational inhibition. Exercise has been shown to affect several miRNAs’ expression in healthy subjects, but this has not yet been studied in patients with coronary artery disease (CAD). Since exercise training confers beneficial long-term effects and may also trigger acute coronary events, it is of utmost interest to be able to identify those who are risk for untoward effects. Therefore, we set out to assess miRNA expression in response to maximal ergospirometry in patients with CAD.

Methods

Total RNA was extracted from blood drawn immediately before and 5 min after maximal cycle-ergospirometry (10 male and 10 female CAD patients). A qRT-PCR was performed for 187 target miRNAs associated with endothelial function/dysfunction, cardiovascular disease, myocardial infarction, and sudden cardiac death.

Results

In response to a maximal ergospirometry, 33 miRNAs significantly changed their expression levels. Of these miRNAs 16 were significantly differently expressed between gender. Using multi-variance analysis, nine miRNAs (let-7e-5p; miR-1; miR-19b-1-5p; miR-103a-3p; miR-148b-3p; miR-181b-5p; miR-188-5p; miR-423-5p; miR-874-3p) showed significantly different responses to maximal ergospirometry between genders.

Conclusions

We report for the first time that in patients with CAD, miRNA expression is amenable to maximal ergospirometry and that the extent of changes differs between genders. Affected by exercise and gender were miRNAs that are associated, among others, with pathways for glucose metabolism, oxidative stress, and angiogenesis. Future studies should assess whether disease-specific miRNA expression in response to maximal exercise might serve as a marker for patient outcome.

Keywords: CAD; ergospirometry; exercise; gender; miRNAs
Corresponding author: Prof. Josef Niebauer, MD, PhD, MBA, University Institute of Sports Medicine, Prevention and Rehabilitation and Research Institute of Molecular Sports Medicine and Rehabilitation, Paracelsus Medical University, Lindhofstr. 20, 5020 Salzburg, Austria, Phone: +43(0)5 7255 23200, Fax: +43(0)5 7255 23299, E-mail:

Acknowledgments

We thank all the participants for their time and effort they dedicated to this study.

  1. Research funding: None declared.

  2. Author contributions: BM, EEM, MS, JN contributed to the conception or design of the work. BM, EEM, CS, SD contributed to the acquisition, analysis, or interpretation of data for the work. BM drafted the manuscript. EEM, MS, CS, SD, JN critically revised the manuscript. All gave final approval and agree to be accountable for all aspects of work ensuring integrity and accuracy. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: Ethics Committee of the State of Salzburg no. 415-E/1734/10-2015, ClinicalTrials.gov: NCT02303379.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2021-0164).

Received: 2020-12-11
Accepted: 2021-05-04
Published Online: 2021-05-12
Published in Print: 2021-09-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2021-0164
Keywords for this article
CAD; ergospirometry; exercise; gender; miRNAs

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Ageing, ACE2 deficiency and bad outcome in COVID-19
  4. Review
  5. Harmonization status of procalcitonin measurements: what do comparison studies and EQA schemes tell us?
  6. Opinion Papers
  7. Role of ACE2 polymorphism in COVID-19: impact of age
  8. The importance of correct stratifications when comparing directly and indirectly estimated reference intervals
  9. General Clinical Chemistry and Laboratory Medicine
  10. The clinical laboratory: a decision maker hub
  11. Simultaneous measurement of 13 circulating vitamin D3 and D2 mono and dihydroxy metabolites using liquid chromatography mass spectrometry
  12. Cerebrospinal fluid hemoglobin levels as markers of blood contamination: relevance for α-synuclein measurement
  13. Novel severe traumatic brain injury blood outcome biomarkers identified with proximity extension assay
  14. Hemoglobin Yamagata [β132(H10)Lys→Asn; (HBB: c.399A>T)]: a mosaic to be put together
  15. Reference Values and Biological Variations
  16. Pediatric reference interval verification for endocrine and fertility hormone assays on the Abbott Alinity system
  17. Hematology and Coagulation
  18. Neonatal lymphocyte subpopulations analysis and maternal preterm premature rupture of membranes: a pilot study
  19. Pro-coagulant imbalance in patients with community acquired pneumonia assessed on admission and one month after hospital discharge
  20. A multi-laboratory assessment of congenital thrombophilia assays performed on the ACL TOP 50 family for harmonisation of thrombophilia testing in a large laboratory network
  21. Cardiovascular Diseases
  22. Exercise-induced changes in miRNA expression in coronary artery disease
  23. Diabetes
  24. Recommendations for proficiency testing criteria for hemoglobin A1c based on the Shanghai Center for Clinical Laboratory’s study
  25. Infectious Diseases
  26. The versatility of external quality assessment for the surveillance of laboratory and in vitro diagnostic performance: SARS-CoV-2 viral genome detection in Austria
  27. Letters to the Editor
  28. Monitoring of the immunogenic response to Pfizer BNT162b2 mRNA COVID-19 vaccination in healthcare workers with Snibe SARS-CoV-2 S-RBD IgG chemiluminescent immunoassay
  29. SARS-CoV-2 antibody assay after vaccination: one size does not fit all
  30. Analysis of leptin-adiponectin ratio and C-reactive protein as potential biomarkers of metabolic syndrome in adolescents
  31. Hb Johnstown is detected on Mindray BC 6800 Plus analyzer
  32. Long-term stability of 25-hydroxyvitamin D: importance of the analytical method and of the patient matrix
  33. Development of chronic myeloid leukemia in a patient previously diagnosed with a JAK2-positive myeloproliferative neoplasm
  34. Acquired Pelger-Huet anomaly in two patients with chronic lymphocytic leukemia treated with venetoclax
  35. Novel variant of MYH9 associated with mild evaluation of MYH9 related disorder in a Chinese family
  36. The successful inclusion of ADA SCID in Tuscany expanded newborn screening program
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