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Monitoring the effectiveness of hypothermia in perinatal asphyxia infants by urinary S100B levels

  • Iliana Bersani , Fabrizio Ferrari , Licia Lugli , Giorgio Ivani , Alessandra Conio , Bashir Moataza , Hanna Aboulgar , Hala Mufeed , Iman Iskander , Maria Kornacka , Darek Gruzfeld , Andrea Dotta , Immacolata Savarese , Natalia Chukhlantseva , Lucia Gabriella Tina , Francesco Nigro , Giovanni Livolti , Fabio Galvano , Laura Serpero , Micaela Colivicchi , Patrizia Ianniello , Francesca Pluchinotta , Luigi Anastasia , Ekaterina Baryshnikova , Diego Gazzolo EMAIL logo and on behalf of the Cooperative Multitask against Brain Injury of Neonates (CoMBINe) Group
Published/Copyright: February 12, 2019
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 57 Issue 7

Abstract

Background

Perinatal asphyxia is a major cause of mortality and morbidity in neonates: The aim of the present study was to investigate, by means of longitudinal assessment of urinary S100B, the effectiveness of hypothermia, in infants complicated by perinatal asphyxia and hypoxic-ischemic encephalopathy.

Methods

We performed a retrospective case-control study in 108 asphyxiated infants, admitted to nine tertiary departments for neonatal intensive care from January 2004 to July 2017, of whom 54 underwent hypothermia treatment and 54 did not. The concentrations of S100B protein in urine were measured using an immunoluminometric assay at first urination and 4, 8, 12, 16, 20, 24, 48, 72, 96, 108 and 120 h after birth. The results were correlated with the achievement of S100B levels within normal ranges at 72 h from hypothermia treatment. Routine laboratory parameters, longitudinal cerebral function monitoring, cerebral ultrasound and neurologic patterns were assessed according to standard protocols.

Results

Higher S100B concentrations were found in hypothermia-treated infants in both moderate (up to 12 h) and severe (up to 24 h) hypoxic-ischemic encephalopathy. S100B levels returned to normal ranges starting from 20 h of hypothermia treatment in moderate and from 36 h in severe hypoxic-ischemic encephalopathy.

Conclusions

The present results offer additional support to the usefulness of longitudinal neuro-biomarkers monitoring in asphyxiated infants treated by hypothermia. The pattern of S100B concentrations during hypothermia supports the need for further investigations aimed at reconsidering the time-window for patient recruitment and treatment, and the optimal duration of the cooling and rewarming phases of the hypothermia procedure.

Keywords: asphyxia; hypothermia; neonate; S100B
Corresponding author: Prof. Diego Gazzolo, Department of Maternal, Fetal and Neonatal Health C. Arrigo Children’s Hospital, Alessandria, Italy; and Neonatal Intensive Care Unit, G. d’Annunzio University, Chieti, Italy

Acknowledgments

This work is part of the I.O. PhD International Program under the auspices of the Italian Society of Neonatology and was partially supported by grants to DG from “I Colori della Vita Foundation”, Italy. We thank Diasorin, Saluggia, Italy, for supporting analysis kits.

  1. Author contribution: Bersani Iliana and Serpero Laura contributed to the conceptualization, investigation and writing – original draft. Ferrari Fabrizio contributed to the conceptualization, investigation and writing – review and editing. Conio Alessandra, Gruszfeld Darek, Savarese Immacolata, Iskander Iman, Tina Lucia Gabriella, Nigro Francesco, Ianniello Patrizia, Baryshnikova Ekaterina and Natalia Chukhlantseva contributed to the investigation. Aboulgar Hanna, Bashir Moataza, Mufeed Said Hala, Kornacka Maria, Ivani Giorgio, Lugli Licia, Andrea Dotta, Livolti Giovanni, Galvano Fabio, Colivicchi Micaela and Pluchinotta Francesca contributed to the investigation and conceptualization. Anastasia Luigi contributed to the conceptualization and supervision. Diego Gazzolo contributed to the project administration, conceptualization, investigation, supervision and writing – review and editing. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work is part of the I.O. PhD International Program under the auspices of the Italian Society of Neonatology and was partially supported by grants to DG from “I Colori della Vita Foundation”, Italy. We thank Diasorin, Saluggia, Italy, for supporting analysis kits.

  3. Employement or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organizations played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2018-10-09
Accepted: 2019-01-18
Published Online: 2019-02-12
Published in Print: 2019-06-26

©2019 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2018-1094
Keywords for this article
asphyxia; hypothermia; neonate; S100B

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Epigenetics in cancer: a promising path to follow?
  4. Reviews
  5. The circulating miRNAs as diagnostic and prognostic markers
  6. Analytical barriers in clinical B-type natriuretic peptide measurement and the promising analytical methods based on mass spectrometry technology
  7. Mini Review
  8. Commutability of reference and control materials: an essential factor for assuring the quality of measurements in Laboratory Medicine
  9. EFLM Paper
  10. Preanalytical challenges – time for solutions
  11. General Clinical Chemistry and Laboratory Medicine
  12. Iron status determination in individuals with Helicobacter pylori infection: conventional vs. new laboratory biomarkers
  13. Harmonizing by reducing inter-run variability: performance evaluation of a quality assurance program for antinuclear antibody detection by indirect immunofluorescence
  14. Increased prevalence of anti-DFS70 antibodies in young females: experience from a large international multi-center study on blood donors
  15. Accuracy of determination of the glomerular filtration marker iohexol by European laboratories as monitored by external quality assessment
  16. Therapeutic drug monitoring (TDM) of 5-fluorouracil (5-FU): new preanalytic aspects
  17. Monitoring the effectiveness of hypothermia in perinatal asphyxia infants by urinary S100B levels
  18. Hematology and Coagulation
  19. Determination of haemoglobin derivatives in aged dried blood spot to estimate haematocrit
  20. Reference Values and Biological Variations
  21. A study of biological and lifestyle factors, including within-subject variation, affecting concentrations of growth differentiation factor 15 in serum
  22. Reference intervals for thyroid-stimulating hormone, free thyroxine, and free triiodothyronine in elderly Chinese persons
  23. Cancer Diagnostics
  24. Extracellular vesicle-associated miRNAs in ovarian cancer – design of an integrated NGS-based workflow for the identification of blood-based biomarkers for platinum-resistance
  25. Evaluating the diagnostic and prognostic value of serum long non-coding RNA CTC-497E21.4 in gastric cancer
  26. A pilot study of new promising non-coding RNA diagnostic biomarkers for early-stage colorectal cancers
  27. Cardiovascular Diseases
  28. Prognostic role of GDF-15 across the spectrum of clinical risk in patients with NSTE-ACS
  29. Acute-phase dynamics and prognostic value of growth differentiation factor-15 in ST-elevation myocardial infarction
  30. Diabetes
  31. Lipid profile and genetic status in a familial hypercholesterolemia pediatric population: exploring the LDL/HDL ratio
  32. Letters to the Editor
  33. The search for a new test of early cancer detection
  34. Need of a dedicated isotopic internal standard for accurate 3-epi-25(OH)D3 quantification by LC-MS/MS
  35. An unexpectedly prolonged severe hyperbilirubinemia in a patient with pre-existing hepatitis A: a role of genetic predisposition?
  36. Creatine kinase elevation: a neglected clue to the diagnosis of polymyositis. A case report
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  42. Requirement of a reference measurement system for the tissue factor-induced coagulation time and the international normalized ratio
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