Antibodies to phosphatidylserine/prothrombin (aPS/PT) enhanced the diagnostic performance in Chinese patients with antiphospholipid syndrome
-
Shulan Zhang
Abstract
Background:
Increasing evidence has highlighted the role of non-criteria antiphospholipid antibodies (aPLs) as important supplements to the current criteria aPLs for the diagnosis of antiphospholipid syndrome (APS). In this retrospective study, we evaluated the clinical relevance of antibodies to phosphatidylserine/prothrombin (aPS/PT) in Chinese patients with APS.
Methods:
A total of 441 subjects were tested, including 101 patients with primary APS (PAPS), 140 patients with secondary APS (SAPS), 161 disease controls (DCs) and 39 healthy controls (HCs). Serum IgG/IgM aPS/PT was determined by ELISA.
Results:
The levels of IgG/IgM aPS/PT were significantly increased in patients with APS compared with DCs and HCs. IgG and IgM aPS/PT were present in 29.7% and 54.5% of PAPS, and 42.1% and 53.6% of SAPS, respectively. For diagnosis of APS, IgG aCL exhibited the highest positive likelihood ratio (LR+) of 21.60, followed by LA (13.84), IgG aβ2GP1 (9.19) and IgG aPS/PT (8.49). aPS/PT was detected in 13.3% of seronegative PAPS patients and 31.3% of seronegative SAPS patients. LA exhibited the highest OR of 3.64 in identifying patients with thrombosis, followed by IgG aCL (OR, 2.63), IgG aPS/PT (OR, 2.55) and IgG aβ2GP1 (OR, 2.33). LA and IgG aCL were correlated with both arterial and venous thrombosis, whereas IgG aPS/PT and IgG aβ2GP1 correlated with venous or arterial thrombosis, respectively.
Conclusions:
Our findings suggest that the inclusion of IgG/IgM aPS/PT may enhance the diagnostic performance for APS, especially in those in whom APS is highly suspected, but conventional aPLs are repeatedly negative. In addition, IgG aPS/PT may contribute to identify patients at risk of thrombosis.
Acknowledgments
We thank all the people from the Department of Rheumatology and Clinical Immunology, PUMCH, for their assistance during this study.
Author contributions: SZ, FZ and YL designed the study. SZ, ZW, WZ, JZ, XZ, GLN and YL performed the experiments and analyzed the data. SZ, GLN, FZ and YL wrote the manuscript. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was supported in part by the National Natural Science Foundation of China, Funder ID: 10.13039/501100001809; grant nos. 81373188, 81671618/81172857 (to YL) and 81771661 (to SZ); Chinese Academy of Medical Sciences (CAMS) Initiative for Innovative Medicine (CAMS-I2M) grant no. 2017-I2M-3-001 (to SZ and YL); and The National Key Research and Development Program of China grant no. 2016YFC0903900 (to YL).
Employment or leadership: Gary L. Norman is an employee of Inova Diagnostics.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Editorial
- Developments in laboratory testing for autoimmune diseases
- Reviews
- Cerebrospinal fluid microRNAs as diagnostic biomarkers in brain tumors
- HCV core antigen comes of age: a new opportunity for the diagnosis of hepatitis C virus infection
- Mini Reviews
- Insulin autoimmune syndrome (Hirata’s disease) in an Italian patient: a case report and review of the literature
- The natural history of autoimmune Addison’s disease with a non-classical presentation: a case report and review of literature
- Opinion Paper
- Quality and future of clinical laboratories: the Vico’s whole cyclical theory of the recurring cycles
- General Clinical Chemistry and Laboratory Medicine
- Screening for connective tissue disease-associated antibodies by automated immunoassay
- Analysis of anti-ganglioside antibodies by a line immunoassay in patients with chronic-inflammatory demyelinating polyneuropathies (CIDP)
- Detection of autoantibodies to the p200-epitope of SSA/Ro52 antigen. A comparison of two laboratory assays
- A new ELISA for autoantibodies to steroid 21-hydroxylase
- Antibodies to phosphatidylserine/prothrombin (aPS/PT) enhanced the diagnostic performance in Chinese patients with antiphospholipid syndrome
- Borderline positive antineutrophil cytoplasmic antibodies (ANCA)-PR3/MPO detection in a large cohort tertiary center: lessons learnt from a real-life experience
- Reference Values and Biological Variations
- Short- and medium-term biological variation estimates of red blood cell and reticulocyte parameters in healthy subjects
- Pediatric reference intervals for 1,25-dihydroxyvitamin D using the DiaSorin LIAISON XL assay in the healthy CALIPER cohort
- Reference intervals for biochemical, haemostatic and haematological parameters in healthy Chinese women during early and late pregnancy
- Cancer Diagnostics
- Use of circulating tumor cells in prospective clinical trials for NSCLC patients – standardization of the pre-analytical conditions
- High microRNA-28-5p expression in colorectal adenocarcinoma predicts short-term relapse of node-negative patients and poor overall survival of patients with non-metastatic disease
- Infectious Diseases
- Diagnosis of acute pediatric appendicitis from children with inflammatory diseases by combination of metabolic markers and inflammatory response variables
- Letters to the Editor
- Repeat requesting on hemolysed plasma potassium requests
- A case report of insulin autoimmune syndrome in a Central European individual
- Traceability of alkaline phosphatase measurement may also vary considerably using the same analytical system: the case of Abbott Architect
- Abiraterone acetate: a potential source of interference in testosterone assays
- Falsely elevated plasma testosterone concentrations in neonates: importance of LC-MS/MS measurements
- Rational approach to the primary evaluation of thyroid disease in paediatrics. Full thyroid profile vs. thyroid-stimulating hormone and free thyroxine only
- Diagnostic performance of the N-terminal pro-brain natriuretic peptide to detect an increased left atrial pressure in patients with persistent atrial fibrillation
- Is the DNA of placental origin packaged in exosomes isolated from plasma and serum of pregnant women?
- Gamma heavy chain disease evolving during the progression of chronic lymphocytic leukemia
- Congress Abstracts
- ISMD2018 Twelfth International Symposium on Molecular Diagnostics
