Performance of the XN-2000 WPC channel-flagging to differentiate reactive and neoplastic leukocytosis
-
Peter Schuff-Werner
,
Peter Kohlschein
Abstract
Background:
The distinction between reactive and neoplastic leukocytes, especially atypical lymphocytes suspected to be reactive or neoplastic, is a particular challenge in automated hematological cell differentiation. The aim of the study was to evaluate the performance of the XN analyzer supplemented with the WPC channel for differentiating between reactive and neoplastic leukocytosis.
Methods:
Blood samples of 253 patients with viral infections, lymphoma or leukemia were analyzed by the Sysmex XN-2000 analyzer equipped with the WPC channel. The results were compared to routine leukocyte differentiation using the routine Sysmex XE-2100 analyzer and automated digital microscopy (DM96). The combined information from standard morphology, immune phenotyping and clinical diagnosis served as a reference.
Results:
The XN WPC channel demonstrated an excellent performance for differentiating neoplastic (AUC=0.933) and reactive leukocytosis (AUC=0.900) as compared to morphological smear examination (AUC=0.949 and AUC=0.968, respectively) or to the differentiation results of our routine hematology analyzer (AUC=0.630 and AUC=0.635, respectively).
Conclusions:
Our data show that the combined WDF/WPC of the Sysmex XN-Series analyzer is advantageous in the automated differentiation of neoplastic and reactive leukocytosis, thus supporting the correct diagnostic decision in the daily laboratory routine.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. PSW and PK contributed equally to this paper.
Research funding: The study was supported by a grant from Sysmex Europe, Norderstedt, to cover the cost of reagents. Travel costs declared by PSW for participation at scientific meetings were covered in part by Sysmex Europe.
Employment or leadership: AM and JL are full time employees of Sysmex Europe.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2016 Walter de Gruyter GmbH, Berlin/Boston
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- Frontmatter
- Editorials
- The Theranos phenomenon, scientific transparency and freedom of speech
- Holotranscobalamin: in the middle of difficultly lies opportunity
- Review
- Laboratory and clinical risk assessment to treat myelodysplatic syndromes
- Mini Review
- Quantitative nucleic acid amplification by digital PCR for clinical viral diagnostics
- Genetics and Molecular Diagnostics
- Hybrid minigene splicing assay verified the pathogenicity of a novel splice site variant in the dystrophin gene of a Chinese patient with typical Duchenne muscular dystrophy phenotype
- General Clinical Chemistry and Laboratory Medicine
- Prospective validation of an automated chemiluminescence-based assay of renin and aldosterone for the work-up of arterial hypertension
- Sex steroid hormone stability in serum tubes with and without separator gels
- Reduced absorption and enhanced synthesis of cholesterol in patients with cystic fibrosis: a preliminary study of plasma sterols
- An International Standard for holotranscobalamin (holoTC): international collaborative study to assign a holoTC value to the International Standard for vitamin B12 and serum folate
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- Hematology and Coagulation
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