Abstract
Background: Correct cell enumeration and differential analysis of body fluids are important in the diagnosis and management of several diseases. Currently, microscopic analysis is still considered the “gold standard”. The aim of the present study was to evaluate the analytical performance of the CELL-DYN Sapphire hematology analyzer for automated differentiation of cells in serous fluids and to explore whether manual analysis of the raw data files could improve the differential count compared with reference microscopy.
Methods: A total of 105 serous fluids (39 peritoneal and 66 pleural effusions) were analyzed by the CELL-DYN Sapphire using standard whole-blood algorithm. Additionally, we performed optimized manual gating of the Sapphire raw data file using standard flow cytometry software.
Results: The standard Sapphire algorithm showed substantial deviations from the reference microscopic differentiation: polymorphonuclear cell counts were too high because they contained some monocytic cells. However, when optimized manual gating strategy is used, a good correlation and negligible bias were found.
Conclusions: We have demonstrated that with a modified algorithm, CELL-DYN Sapphire will provide reliable identification and enumeration of blood cells in peritoneal and pleural fluids
References
1. Rimola A, Garcia-Tsao G, Navasa M, Piddock LJ, Planas R, Bernard B, et al. Diagnosis, treatment and prophylaxis of spontaneous bacterial peritonitis: a consensus document. International Ascites Club. J Hepatol 2000;32:142–53.10.1016/S0168-8278(00)80201-9Suche in Google Scholar
2. Kleine TO, Nebe CT, Löwer C, Lehmitz R, Kruse R, Geilenkeuser W-J, et al. Modifications of haematology analyzers to improve cell counting and leukocyte differentiating in cerebrospinal fluid controls of the Joint German Society for Clinical Chemistry and Laboratory Medicine. Cytometry 2009;75A:688–91.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000268289300007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=b7bc2757938ac7a7a821505f8243d9f310.1002/cyto.a.20753Suche in Google Scholar PubMed
3. Rabinovitch A. Hematology analyzers and body fluid analysis. Am J Clin Pathol 2010;134:167–8.10.1309/AJCPOUSCFIW60DYCSuche in Google Scholar PubMed
4. De Smet D, Van Moer G, Martens GA, Nanos N, Smet L, Jochmans K, et al. Use of the Cell-Dyn Sapphire hematology analyzer for automated counting of blood cells in body fluids. Am J Clin Pathol 2010;133:291–9.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000273796900015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=b7bc2757938ac7a7a821505f8243d9f310.1309/AJCPY7J7OLASZAPCSuche in Google Scholar PubMed
5. Müller R, Mellors I, Johannessen B, Aarsand AK, Kiefer P, Hardy J, et al. European multi-center evaluation of the Abbott Cell-Dyn Sapphire hematology analyzer. Lab Hematol 2006;12:15–31.10.1532/LH96.05041Suche in Google Scholar PubMed
6. Molero T, Lemes A, De la Iglesia S, Scott CS. Monoclonal antibody fluorescence for routine lymphocyte subpopulation analysis with the Abbott CELL-DYN Sapphire haematology analyser. Int J Lab Hematol 2007;29:446–53.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000250727100008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=b7bc2757938ac7a7a821505f8243d9f310.1111/j.1751-553X.2007.00934.xSuche in Google Scholar PubMed
©2013 by Walter de Gruyter Berlin Boston
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Artikel in diesem Heft
- Letters to the Editor
- Evaluation of a novel room temperature RNA storage tube for use in a real-time quantitative PCR assay
- Evaluation of the Universal Master Mix (STAT-NAT DNA-Mix) for reliable molecular testing
- HbA1c: performance of the Sebia Capillarys 2 Flex Piercing
- A case of monoclonal gammopathy of undetermined significance (MGUS): type IgD-lambda
- Evidence-based use of serum protein electrophoresis in laboratory medicine
- Plasma volume shifts during multiday racing
- Structured handoff at shift change in a clinical laboratory increases patient safety
- Instrument-dependent interference of Howell-Jolly bodies in reticulocyte enumeration
- Reply to Gore et al.: Plasma volume shift during multiday racing
- Recommendations for appropriate serum electrophoresis requests: the Italian approach
- Masthead
- Masthead
- Editorials
- Biomarkers for sepsis: an unfinished journey
- Laboratory demand management of repetitive testing – time for harmonisation and an evidenced based approach
- Reviews
- Non-invasive prenatal diagnostics of aneuploidy using next-generation DNA sequencing technologies, and clinical considerations
- The diagnostic utility of brain natriuretic peptide in heart failure patients presenting with acute dyspnea: a meta-analysis
- Opinion Paper
- Opinion paper on innovative approach of biomarkers for infectious diseases and sepsis management in the emergency department
- Genetics and Molecular Diagnostics
- Microarray with LNA-probes for genotyping of polymorphic variants of Gilbert’s syndrome gene UGT1A1(TA)n
- Selection of the optimal manual method of cell free fetal DNA isolation from maternal plasma
- A multiplex assay to rapidly exclude HLA-DQ2.5 and HLA-DQ8 expression in patients at risk for celiac disease
- Low cost biosensor-based molecular differential diagnosis of α-thalassemia (Southeast Asia deletion)
- General Clinical Chemistry and Laboratory Medicine
- Managing laboratory test ordering through test frequency filtering
- Critical review of laboratory investigations in clinical practice guidelines: proposals for the description of investigation
- Long-term stability of laboratory tests and practical implications for quality management
- Coffee consumption, serum γ-glutamyltransferase, and glucose tolerance status in middle-aged Japanese men
- Biological variation and prognosis usefulness of new biomarkers in liver transplantation
- Switching between parathormone (PTH) assays: the impact on the diagnosis of renal osteodystrophy
- A comparison between two different in vitro basophil activation tests for gluten- and cow’s milk protein sensitivity in irritable bowel syndrome (IBS)-like patients
- A proficiency testing program of hemoglobin analysis in prevention and control of severe hemoglobinopathies in Thailand
- Advancing haemostasis automation – successful implementation of robotic centrifugation and sample processing in a tertiary service hospital
- ADAM12s and PP13 as first trimester screening markers for adverse pregnancy outcome
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- Cancer Diagnostics
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- Do laboratories follow heart failure recommendations and guidelines and did we improve? The CARdiac MArker Guideline Uptake in Europe (CARMAGUE)
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- Additional diagnostic and prognostic value of copeptin ultra-sensitive for diagnosis of non-ST-elevation myocardial infarction in older patients presenting to the emergency department1)
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