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Validation of the body fluid module on the new Sysmex XN-1000 for counting blood cells in cerebrospinal fluid and other body fluids

  • Chérina Fleming , Rob Brouwer , Jan Lindemans and Robert de Jonge EMAIL logo
Published/Copyright: May 7, 2012
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 50 Issue 10

Abstract

Background: We evaluated the body fluid (BF) module on the new Sysmex XN-1000 for counting blood cells.

Methods: One hundred and eighty-seven BF samples [73 cerebrospinal fluid (CSF), 48 continuous ambulatory peritoneal dialysis (CAPD), 46 ascites, and 20 pleural fluid] were used for method comparison between the XN-1000 and manual microscopy (Fuchs-Rosenthal chamber and stained cytospin slides) for counting red blood cells (RBCs) and white blood cells (WBCs) (differential).

Results: Good agreement was found for counting WBCs (y=1.06x+0.09, n=67, R2=0.96) and mononuclear cells (MNs) (y=1.04x–0.01, n=40, R2=0.93) in CSF. However, the XN-1000 systematically counted more polymorphonuclear cells (PMNs) (y=1.48x+0.18, n=40, R2=0.99) compared to manual microscopy. Excellent correlation for RBCs >1×109/L (y=0.99x+116.56, n=26, R2=0.99) in CSF was found. For other fluids (CAPD, ascites and pleural fluid) excellent agreement was found for counting WBCs (y=1.06x+0.26, n=109, R2=0.98), MNs (y=1.06x–0.41, n=93, R2=0.96), PMNs (y=1.06x+0.81, n=93, R2=0.98) and RBCs (y=1.04x+110.04, n=43, R2=0.98). By using BF XN-check, the lower limit of quantitation (LLoQ) for WBC was defined at 5×106/L. Linearity was excellent for both the WBCs (R2=0.99) and RBCs (R2=0.99) and carry-over never exceeded 0.05%.

Conclusions: The BF module on the XN-1000 is a suitable tool for fast and accurate quantification of WBC (differential) and RBC counts in CSF and other BFs in a diagnostic setting.

Keywords: body fluid; cerebrospinal fluid; counting chamber; hematology analyzer; leukocytes; Sysmex XN-1000
Corresponding author: Dr. Robert de Jonge, Department of Clinical Chemistry, L-137, Erasmus MC, University Medical Center Rotterdam, s-Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands Phone: +31 10 7033473, Fax: +31 10 4367894
Received: 2011-12-15
Accepted: 2012-3-22
Published Online: 2012-05-07
Published in Print: 2012-10-01

©2012 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/cclm-2011-0927
Keywords for this article
body fluid; cerebrospinal fluid; counting chamber; hematology analyzer; leukocytes; Sysmex XN-1000

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Editorials
  4. Cancer epigenomics: moving slowly, but at a steady pace from laboratory bench to clinical practice
  5. Laboratory networking and sample quality: a still relevant issue for patient safety
  6. Reviews
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  9. Global DNA hypomethylation in cancer: review of validated methods and clinical significance
  10. Genetics and Molecular Diagnostics
  11. δ0-Thalassemia in cis of βKnossos globin gene: first homozygous description in thalassemia intermedia Libyans and first combination with codon 39 (C→T) in thalassemia intermedia Tunisian patients
  12. Genetic-based reference values for angiotensin-converting enzyme (ACE) according to I/D polymorphism in a Spanish population sample
  13. General Clinical Chemistry and Laboratory Medicine
  14. Effects of sample transportation on commonly requested laboratory tests
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  16. Information comparison of the effects of drugs on laboratory tests in drug labels and Young’s book
  17. First evaluation of Capillarys 2 Flex Piercing® (Sebia) as a new analyzer for HbA1c assay by capillary electrophoresis
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  21. Cancer Diagnostics
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  23. Infectious Diseases
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  28. Serum cystatin C as a marker for early detection of chronic kidney disease and grade 2 nephropathy in Japanese patients with type 2 diabetes
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  31. Application of quality specification based on biological variation in planning quality control strategy
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  33. Comparison of relationships between FT4 and log TSH in Access DXI 800 Unicel, Modular E170 and ADVIA Centaur XP Analyzer
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