Characterization of cellular development and fatty acid accumulation in thraustochytrid Aurantiochytrium strains of Taiwan
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Natarajan Velmurugan
Natarajan Velmurugan obtained his PhD from Chonbuk National University, South Korea in 2010. During 2010–2015, he worked as post-doctoral associate at the Korea Advanced Institute of Science and Technology, South Korea and University of Minnesota, USA on various projects related to microbial bioengineering. In 2016, he joined as Scientist at CSIR-North East Institute of Science and Technology, currently he is a Senior Scientist at CSIR-NEIST, and his research is focused on microbial and plant bioengineering for beneficial products.
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Yesupatham Sathishkumar
Yesupatham Sathishkumar obtained his BSc and MSc degrees from the Christian Medical College-Vellore and University of Madras-Chennai in 2007 and 2011, respectively. He obtained his PhD degree from the Chonbuk National University, South Korea in 2015. He studies the biology of fungi using microscopy and metabolic changes in fungi under different stress conditions.
Shashanka Sonowal obtained his BSc and MSc degrees from the Guwahati University in 2012 and 2014, respectively. He is currently pursuing his PhD degree in Academy of Scientific and Innovative Research, India. He studies the evolutionary relationship between plant and microbes, and various microorganisms for the production of commercially important compounds.
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Ka-Lai Pang
Ka-Lai Pang obtained his BSc and PhD degrees from the City University of Hong Kong in 1998 and 2001, respectively. Prof. Pang studies the biology of marine fungi and fungus-like organisms and endophytic fungi associated with mangrove plants and macroalgae.
Yang Soo Lee obtained his PhD from University of Portsmouth, UK. He has worked as a post-doc researcher at Korea Research Institute of Chemical Institute (South Korea) and Nanjing Forest University (China) in 1998–2000 and as a chevening scholar at Liverpool John Moore University (UK) in 2003. He has been the Head of Functional Food Center, Chonbuk National University since 2009. He pioneered astromicrobiology research in Korea and established the astromicrobiological research laboratory at the Chonbuk National University, South Korea.
Abstract
Long-chain saturated and polyunsaturated fatty acids of two new thraustochytrid isolates cultured from Taiwan mangroves, Aurantiochytrium sp. IMB169 and Aurantiochytrium sp. IMB171, were characterized through their cell growth and development in relation to their intracellular lipid accumulation using transmission electron microscopy. Flow cytometry in combination with the lipophilic fluorescent dye BODIPY 505/515 was used to stain and characterize intracellular lipid bodies in the two isolates. The transmission electron microscopy and flow cytometry analyses revealed a progressive accumulation of lipid products in IMB169 and IMB171. Further, selective BODIPY stained cells were successfully separated and enriched using flow cytometry at single cell level. Among the two isolates, IMB169 was found to produce a high level of docosahexaenoic acid. The qualitative and analytical results obtained using electron microscopy and flow cytometry studies were validated by gas chromatography (GC). In addition, a quantitative baseline was established using cell growth, flow cytometry and GC analyses for developing an efficient bioprocessing methodology to selectively enrich thraustochytrids phenotypes with desirable characteristics.
Funding source: Chonbuk National University
About the authors
Natarajan Velmurugan obtained his PhD from Chonbuk National University, South Korea in 2010. During 2010–2015, he worked as post-doctoral associate at the Korea Advanced Institute of Science and Technology, South Korea and University of Minnesota, USA on various projects related to microbial bioengineering. In 2016, he joined as Scientist at CSIR-North East Institute of Science and Technology, currently he is a Senior Scientist at CSIR-NEIST, and his research is focused on microbial and plant bioengineering for beneficial products.
Yesupatham Sathishkumar obtained his BSc and MSc degrees from the Christian Medical College-Vellore and University of Madras-Chennai in 2007 and 2011, respectively. He obtained his PhD degree from the Chonbuk National University, South Korea in 2015. He studies the biology of fungi using microscopy and metabolic changes in fungi under different stress conditions.
Shashanka Sonowal obtained his BSc and MSc degrees from the Guwahati University in 2012 and 2014, respectively. He is currently pursuing his PhD degree in Academy of Scientific and Innovative Research, India. He studies the evolutionary relationship between plant and microbes, and various microorganisms for the production of commercially important compounds.
Ka-Lai Pang obtained his BSc and PhD degrees from the City University of Hong Kong in 1998 and 2001, respectively. Prof. Pang studies the biology of marine fungi and fungus-like organisms and endophytic fungi associated with mangrove plants and macroalgae.
Yang Soo Lee obtained his PhD from University of Portsmouth, UK. He has worked as a post-doc researcher at Korea Research Institute of Chemical Institute (South Korea) and Nanjing Forest University (China) in 1998–2000 and as a chevening scholar at Liverpool John Moore University (UK) in 2003. He has been the Head of Functional Food Center, Chonbuk National University since 2009. He pioneered astromicrobiology research in Korea and established the astromicrobiological research laboratory at the Chonbuk National University, South Korea.
Acknowledgments
The authors wish to thank the Director, CSIR-NEIST, Jorhat, Assam for his kind encouragement for preparation of this manuscript.
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: The authors wish to thank Chonbuk National University for the financial support.
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- In this issue
- Editorial
- Marine zoosporic organisms: Labyrinthulomycota and Oomycota
- Research Articles
- Employing marine invertebrate cell culture media for isolation and cultivation of thraustochytrids
- Chitin localisation and retention in the exit tubes of the holocarpic oomycete Anisolpidium rosenvingei
- Occurrence of thraustochytrids: the fungoid protists vis-a-vis marine macroalgae (seaweeds) along the coast of Goa, India
- Characterization of cellular development and fatty acid accumulation in thraustochytrid Aurantiochytrium strains of Taiwan
- Thraustochytrids from the Red Sea mangroves in Saudi Arabia and their abilities to produce docosahexaenoic acid
- Palmitic acid and long-chain polyunsaturated fatty acids dominate in mycelia of mangrove Halophytophthora and Salispina species in Taiwan
Artikel in diesem Heft
- Frontmatter
- In this issue
- Editorial
- Marine zoosporic organisms: Labyrinthulomycota and Oomycota
- Research Articles
- Employing marine invertebrate cell culture media for isolation and cultivation of thraustochytrids
- Chitin localisation and retention in the exit tubes of the holocarpic oomycete Anisolpidium rosenvingei
- Occurrence of thraustochytrids: the fungoid protists vis-a-vis marine macroalgae (seaweeds) along the coast of Goa, India
- Characterization of cellular development and fatty acid accumulation in thraustochytrid Aurantiochytrium strains of Taiwan
- Thraustochytrids from the Red Sea mangroves in Saudi Arabia and their abilities to produce docosahexaenoic acid
- Palmitic acid and long-chain polyunsaturated fatty acids dominate in mycelia of mangrove Halophytophthora and Salispina species in Taiwan
